##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_1_ATCACG_L002_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22286994
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285918190672103	33.0	33.0	33.0	27.0	33.0
2	31.018360035453863	33.0	33.0	33.0	27.0	33.0
3	30.94930868649222	33.0	33.0	33.0	27.0	33.0
4	34.5342032218432	37.0	37.0	37.0	33.0	37.0
5	34.564079211400156	37.0	37.0	37.0	33.0	37.0
6	34.91714669999911	37.0	37.0	37.0	33.0	37.0
7	34.525923639589976	37.0	37.0	37.0	33.0	37.0
8	34.823182570067544	37.0	37.0	37.0	33.0	37.0
9	34.44411758714522	37.0	37.0	37.0	33.0	37.0
10-11	34.531758096224195	37.0	37.0	37.0	33.0	37.0
12-13	34.45316263826337	37.0	37.0	37.0	27.0	37.0
14-15	36.23212132152052	40.0	37.0	40.0	27.0	40.0
16-17	36.2037138117415	40.0	37.0	40.0	27.0	40.0
18-19	36.21654461341893	40.0	37.0	40.0	27.0	40.0
20-21	36.1507700859075	40.0	37.0	40.0	27.0	40.0
22-23	36.551597267895346	40.0	37.0	40.0	27.0	40.0
24-25	36.16756064097294	40.0	37.0	40.0	27.0	40.0
26-27	36.14300957320668	40.0	37.0	40.0	27.0	40.0
28-29	35.85902327159957	40.0	37.0	40.0	27.0	40.0
30-31	35.85611471425891	40.0	37.0	40.0	27.0	40.0
32-33	35.66577845805496	40.0	37.0	40.0	27.0	40.0
34-35	35.56611050821838	40.0	37.0	40.0	27.0	40.0
36-37	35.55158317896078	40.0	37.0	40.0	27.0	40.0
38-39	35.582108112022645	40.0	37.0	40.0	27.0	40.0
40-41	35.53768971715073	40.0	37.0	40.0	27.0	40.0
42-43	35.324165093776216	40.0	37.0	40.0	22.0	40.0
44-45	35.335665388522116	37.0	37.0	40.0	22.0	40.0
46-47	34.922056110393356	37.0	37.0	40.0	22.0	40.0
48-49	34.73995799971948	37.0	37.0	40.0	22.0	40.0
50-51	34.49758125748138	37.0	33.0	40.0	22.0	40.0
52-53	34.37943308998962	37.0	33.0	40.0	22.0	40.0
54-55	34.15259500675596	37.0	33.0	40.0	22.0	40.0
56-57	34.11465406685173	37.0	33.0	40.0	22.0	40.0
58-59	33.73252447144734	37.0	33.0	38.5	22.0	40.0
60-61	34.175658951584055	37.0	33.0	37.0	24.5	40.0
62-63	34.153306834470364	37.0	33.0	37.0	27.0	40.0
64-65	33.96079518395348	37.0	33.0	37.0	27.0	40.0
66-67	33.9040047975963	37.0	33.0	37.0	24.5	40.0
68-69	33.55372628987112	37.0	33.0	37.0	22.0	38.5
70-71	33.128606262468594	37.0	33.0	37.0	22.0	37.0
72-73	32.93517286359928	37.0	33.0	37.0	22.0	37.0
74-75	32.80501473191046	37.0	33.0	37.0	22.0	37.0
76-77	32.695832533539516	37.0	33.0	37.0	22.0	37.0
78-79	32.6123982220303	37.0	33.0	37.0	22.0	37.0
80-81	32.52201198600403	37.0	33.0	37.0	22.0	37.0
82-83	32.427078366871726	37.0	33.0	37.0	22.0	37.0
84-85	32.35341518017189	37.0	33.0	37.0	22.0	37.0
86-87	32.269512299415524	37.0	33.0	37.0	22.0	37.0
88-89	32.163514200255094	37.0	33.0	37.0	22.0	37.0
90-91	32.110351647243235	37.0	33.0	37.0	15.0	37.0
92-93	32.02396996651949	37.0	33.0	37.0	15.0	37.0
94-95	31.978565750051352	37.0	33.0	37.0	15.0	37.0
96-97	31.869109939186952	37.0	33.0	37.0	15.0	37.0
98-99	31.808411242000602	37.0	33.0	37.0	15.0	37.0
100-101	31.219281388957164	35.0	30.0	37.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.15468826696785243
2201	2	0.2279079672906832
2201	3	0.28256486272452364
2201	4	0.32892728108036096
2201	5	0.38867051307393297
2201	6	0.28607822661099647
2201	7	0.283410220904166
2201	8	0.2557112146267997
2201	9	0.3051943824595398
2201	10-11	0.3551212095871392
2201	12-13	0.3070471842082654
2201	14-15	0.5252598913925155
2201	16-17	0.5960570516309573
2201	18-19	0.5808638776369932
2201	20-21	0.5670821609633805
2201	22-23	0.5801526526873602
2201	24-25	0.6659707295702404
2201	26-27	0.6150836722530428
2201	28-29	0.7438595857145387
2201	30-31	0.6754714970911451
2201	32-33	0.6721884905875726
2201	34-35	0.6803233460272793
2201	36-37	0.6786480623881914
2201	38-39	0.6324007543649515
2201	40-41	0.6556632458370117
2201	42-43	0.6732468538295322
2201	44-45	0.6199880547716532
2201	46-47	0.6778723739826589
2201	48-49	0.6591924360883539
2201	50-51	0.7667254200484876
2201	52-53	0.6829608266196843
2201	54-55	0.6664691850823985
2201	56-57	0.6519479663500363
2201	58-59	0.6675262459683964
2201	60-61	0.6027386199030218
2201	62-63	0.6737825883763193
2201	64-65	0.7011226916507667
2201	66-67	0.6855748719727686
2201	68-69	0.7194473429890778
2201	70-71	0.6752270034190104
2201	72-73	0.6751042711059512
2201	74-75	0.6502106069303579
2201	76-77	0.6815142275705739
2201	78-79	0.6644165756946521
2201	80-81	0.6970828686991197
2201	82-83	0.6322825856365171
2201	84-85	0.6336837002200113
2201	86-87	0.6299731710317715
2201	88-89	0.6376123006855323
2201	90-91	0.6627783304751986
2201	92-93	0.6854174141453697
2201	94-95	0.7074708427866199
2201	96-97	0.675529371346741
2201	98-99	0.6813888898657758
2201	100-101	0.8024806404469835
1101	1	0.2573243635600342
1101	2	0.2825276891775097
1101	3	0.33214560052460484
1101	4	0.39765254463772237
1101	5	0.2710330409881365
1101	6	0.3433378835596912
1101	7	0.4186241862533251
1101	8	0.3875767256519467
1101	9	0.423573353504473
1101	10-11	0.39985832677775335
1101	12-13	0.40892642533455614
1101	14-15	0.6439533675064979
1101	16-17	0.4276558300764748
1101	18-19	0.5809703482491599
1101	20-21	0.5911283984854165
1101	22-23	0.6189916355573217
1101	24-25	0.5960754259058092
1101	26-27	0.5998142833393842
1101	28-29	0.5478540232557805
1101	30-31	0.5259829436652765
1101	32-33	0.5356193835980889
1101	34-35	0.6196077348272055
1101	36-37	0.6438346770585781
1101	38-39	0.605633174993983
1101	40-41	0.5155293301337878
1101	42-43	0.5453622910604423
1101	44-45	0.6427349915504337
1101	46-47	0.6103782015163475
1101	48-49	0.6823270425365493
1101	50-51	0.655964799522792
1101	52-53	0.6233968510424148
1101	54-55	0.6563948151320318
1101	56-57	0.6854029019954737
1101	58-59	0.7108042606835809
1101	60-61	0.6910606355653357
1101	62-63	0.6836637394385647
1101	64-65	0.6792184294926074
1101	66-67	0.6508853921027722
1101	68-69	0.7118522155727618
1101	70-71	0.6166285966745448
1101	72-73	0.5883975559530228
1101	74-75	0.6099448617925063
1101	76-77	0.5692731605142782
1101	78-79	0.6254696061626319
1101	80-81	0.5808817293225346
1101	82-83	0.5493400263434438
1101	84-85	0.39047927420494943
1101	86-87	0.5103164174063224
1101	88-89	0.644127907506423
1101	90-91	0.6121887772737225
1101	92-93	0.6459822792875016
1101	94-95	0.5885792941253598
1101	96-97	0.6580984352977097
1101	98-99	0.6870391513987748
1101	100-101	0.6351821625908478
2202	1	0.19704676832076373
2202	2	0.24853086279902925
2202	3	0.3056643010104523
2202	4	0.29807613803163235
2202	5	0.3345628063170807
2202	6	0.3126035957322202
2202	7	0.36831335236894347
2202	8	0.3671004538694973
2202	9	0.3764658164966974
2202	10-11	0.3723316336911964
2202	12-13	0.4174510982161408
2202	14-15	0.5405044620938639
2202	16-17	0.6021498282888871
2202	18-19	0.6012822699496283
2202	20-21	0.5855854402365281
2202	22-23	0.5868575524845454
2202	24-25	0.5162066790366779
2202	26-27	0.5086246363053704
2202	28-29	0.6073029064484956
2202	30-31	0.5429541926073895
2202	32-33	0.6065439648434108
2202	34-35	0.6006539133245283
2202	36-37	0.624037179651225
2202	38-39	0.6015629650826142
2202	40-41	0.6127082543717748
2202	42-43	0.5406441504540638
2202	44-45	0.5135725777287661
2202	46-47	0.526261166100717
2202	48-49	0.5632308064163709
2202	50-51	0.5386056810224602
2202	52-53	0.5963414898892765
2202	54-55	0.5668832403213955
2202	56-57	0.5834031837758573
2202	58-59	0.6207135428155581
2202	60-61	0.587579796039492
2202	62-63	0.5787949623488586
2202	64-65	0.656443696017206
2202	66-67	0.596918032156168
2202	68-69	0.6365957765432881
2202	70-71	0.5857244832330153
2202	72-73	0.5240592702836437
2202	74-75	0.47469593840206414
2202	76-77	0.47680898563562124
2202	78-79	0.5237928898149633
2202	80-81	0.5557586141253026
2202	82-83	0.44602978975471785
2202	84-85	0.46445626753634883
2202	86-87	0.5061050353481278
2202	88-89	0.3606245893557798
2202	90-91	0.2553188653359797
2202	92-93	0.39933279086825024
2202	94-95	0.44534266295708846
2202	96-97	0.45929350141054215
2202	98-99	0.5179079500728356
2202	100-101	0.5544167463244811
1102	1	0.19974209640623997
1102	2	0.1914555493436758
1102	3	0.2025854888470917
1102	4	0.26340407882087646
1102	5	0.25633339228107843
1102	6	0.25776059385203354
1102	7	0.2620029772085246
1102	8	0.255983958609427
1102	9	0.25648873462246513
1102	10-11	0.26133025113643527
1102	12-13	0.25868590130917823
1102	14-15	0.34819870039996204
1102	16-17	0.427683716803152
1102	18-19	0.32508128066431397
1102	20-21	0.40267549667962754
1102	22-23	0.4404622282623336
1102	24-25	0.40798686148330177
1102	26-27	0.4580451073550762
1102	28-29	0.44981308293697
1102	30-31	0.44699305871072426
1102	32-33	0.4054681341836499
1102	34-35	0.4087699039630692
1102	36-37	0.4491315981390187
1102	38-39	0.4314523455746908
1102	40-41	0.4126596295541134
1102	42-43	0.45500009879448555
1102	44-45	0.4218284490091264
1102	46-47	0.45030286281133414
1102	48-49	0.4324707742651057
1102	50-51	0.45967028158646883
1102	52-53	0.34442834974113623
1102	54-55	0.437434005657515
1102	56-57	0.4183988645875871
1102	58-59	0.5255718656511235
1102	60-61	0.44763128328998647
1102	62-63	0.45566397033646666
1102	64-65	0.43698358084743916
1102	66-67	0.44054383351531357
1102	68-69	0.3966897391104993
1102	70-71	0.408186653046279
1102	72-73	0.3960741423613072
1102	74-75	0.47199401781188755
1102	76-77	0.445917125982632
1102	78-79	0.43943704588983934
1102	80-81	0.4186603088739389
1102	82-83	0.32350364322689984
1102	84-85	0.3032738015537575
1102	86-87	0.3788114151491442
1102	88-89	0.3739383910371856
1102	90-91	0.35702117695799274
1102	92-93	0.4371315631270889
1102	94-95	0.35502032036980324
1102	96-97	0.4424797923719268
1102	98-99	0.4275093744978342
1102	100-101	0.380134044281796
2203	1	0.11821708734973413
2203	2	0.07067780470576679
2203	3	0.12750071236838778
2203	4	0.13774100325871785
2203	5	0.17260018620685003
2203	6	0.20624708960266247
2203	7	0.11926234730535157
2203	8	0.16399351389772931
2203	9	0.15678104937134663
2203	10-11	0.1034904966321264
2203	12-13	0.15811375925566296
2203	14-15	0.21628333244194664
2203	16-17	0.2610056673894334
2203	18-19	0.25982854033369307
2203	20-21	0.20892532494428906
2203	22-23	0.18803481591369575
2203	24-25	0.20987331835059564
2203	26-27	0.2134634925216048
2203	28-29	0.24704217729496492
2203	30-31	0.24956411907189846
2203	32-33	0.24318515535414775
2203	34-35	0.2220092417945736
2203	36-37	0.24566733968835308
2203	38-39	0.20028931032440056
2203	40-41	0.18618471557820016
2203	42-43	0.19091988160869278
2203	44-45	0.2188482034071484
2203	46-47	0.21261974959386265
2203	48-49	0.1771708709344395
2203	50-51	0.2152875084775232
2203	52-53	0.20301914410881494
2203	54-55	0.23840522388892538
2203	56-57	0.2334068936033873
2203	58-59	0.22285540364524792
2203	60-61	0.2564072333242251
2203	62-63	0.25754273327212474
2203	64-65	0.20194413646746057
2203	66-67	0.16864403283572926
2203	68-69	0.14483664365851467
2203	70-71	0.18006878453881114
2203	72-73	0.15680545164816095
2203	74-75	0.17741575664685172
2203	76-77	0.1713061722513416
2203	78-79	0.1849781726824702
2203	80-81	0.0728629849939253
2203	82-83	0.13736097008686698
2203	84-85	0.09854659717967706
2203	86-87	0.15819413115432468
2203	88-89	0.137530677264337
2203	90-91	0.186673120263265
2203	92-93	0.2078811931116391
2203	94-95	0.1432476277756649
2203	96-97	0.008982040394581503
2203	98-99	0.12770666518605012
2203	100-101	0.06170187645205161
1103	1	0.1527376308521191
1103	2	0.1147571947438415
1103	3	0.15126449242156426
1103	4	0.196877673380925
1103	5	0.2109912723118299
1103	6	0.22337230946455122
1103	7	0.19446790145446613
1103	8	0.21585503014816254
1103	9	0.21364095734018917
1103	10-11	0.21654189964693416
1103	12-13	0.2262074482817198
1103	14-15	0.33764457923507507
1103	16-17	0.36625340267963935
1103	18-19	0.309238113132956
1103	20-21	0.31144831679730345
1103	22-23	0.3553838772839484
1103	24-25	0.3442004935262517
1103	26-27	0.39919550933576886
1103	28-29	0.34516980857139146
1103	30-31	0.33349933905490303
1103	32-33	0.32032582917410224
1103	34-35	0.38345901407921446
1103	36-37	0.35642043812958946
1103	38-39	0.32682269872410785
1103	40-41	0.3559938299050742
1103	42-43	0.35944682696705144
1103	44-45	0.3637609371747743
1103	46-47	0.30007015031865336
1103	48-49	0.29221496870263053
1103	50-51	0.4078805881943026
1103	52-53	0.3780436796415785
1103	54-55	0.3649521764046071
1103	56-57	0.37456233819087714
1103	58-59	0.36828097521897263
1103	60-61	0.38476597002498636
1103	62-63	0.42346373674685367
1103	64-65	0.45164315909387653
1103	66-67	0.384763197793923
1103	68-69	0.3835876055812406
1103	70-71	0.268353566799199
1103	72-73	0.3534981943948594
1103	74-75	0.3013699781957939
1103	76-77	0.25691785077814444
1103	78-79	0.22975017886034976
1103	80-81	0.2437677251489916
1103	82-83	0.2646721823164242
1103	84-85	0.12168329995236604
1103	86-87	0.22964877141215823
1103	88-89	0.3564128832452127
1103	90-91	0.2592606701898603
1103	92-93	0.3575908670965404
1103	94-95	0.3950983896133735
1103	96-97	0.2445451404278458
1103	98-99	0.2786873038291908
1103	100-101	0.24975213679064723
2204	1	0.09560865565297405
2204	2	0.09839623639858885
2204	3	0.06642930490123078
2204	4	0.13705091398332314
2204	5	0.13983786163992562
2204	6	0.18294538138675875
2204	7	0.12111929349777029
2204	8	0.14150603640126036
2204	9	0.142091395465215
2204	10-11	0.143139249664614
2204	12-13	0.1145764426801108
2204	14-15	0.17909589215802413
2204	16-17	0.17092866583525534
2204	18-19	0.120120987933241
2204	20-21	0.08342003048680624
2204	22-23	0.0017834499712563456
2204	24-25	-0.17238306929927916
2204	26-27	-0.1149251578340369
2204	28-29	-0.2984940484593892
2204	30-31	-0.14008541332312063
2204	32-33	-0.17288241865155385
2204	34-35	-0.24735194597324295
2204	36-37	-0.17913218965900057
2204	38-39	-0.08036748093325485
2204	40-41	0.0024241519947665324
2204	42-43	0.05241255286291846
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1213	60-61	-2.3547465818378264
1213	62-63	-2.2880649897467507
1213	64-65	-2.1755707427592696
1213	66-67	-1.9132236484960359
1213	68-69	-1.733161466028676
1213	70-71	-1.6322778737619608
1213	72-73	-1.6348814037467818
1213	74-75	-1.589286346374955
1213	76-77	-1.438735027143931
1213	78-79	-1.513448726427761
1213	80-81	-1.501510549796258
1213	82-83	-1.497177071112091
1213	84-85	-1.3822371892834653
1213	86-87	-1.691374398460205
1213	88-89	-1.580989492770378
1213	90-91	-1.7904640522652855
1213	92-93	-1.938930019334471
1213	94-95	-1.9101643303085325
1213	96-97	-1.8864297229369171
1213	98-99	-1.9304802666520935
1213	100-101	-2.1754141408984218
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1471.0
3	8418.0
4	6594.0
5	1696.0
6	1217.0
7	3006.0
8	13650.0
9	62888.0
10	331302.0
11	549880.0
12	219014.0
13	81039.0
14	56103.0
15	51740.0
16	52329.0
17	56412.0
18	63144.0
19	71515.0
20	81483.0
21	93582.0
22	106422.0
23	120776.0
24	135955.0
25	154998.0
26	180113.0
27	213345.0
28	252952.0
29	302188.0
30	368251.0
31	461646.0
32	604793.0
33	816465.0
34	1112253.0
35	1574730.0
36	2773864.0
37	7375796.0
38	3923450.0
39	2514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.816751706597024	12.169587975544486	15.19129248334164	34.822367834516854
2	23.20991853178449	23.753735806091704	34.615845802032055	18.420499860091752
3	23.85549471457474	26.078042257103203	25.423505180992656	24.642957847329406
4	25.237727558787704	34.51496756942856	18.816144131245895	21.431160740537837
5	26.4396400869491	33.65361236458631	21.144312833662323	18.762434714802268
6	21.51711988551319	34.41745392505437	25.866850381233757	18.19857580819869
7	18.928763216151896	16.749495926457435	41.71089909665084	22.610841760739824
8	18.873185293951565	23.791251562182563	28.971175582721813	28.364387561144056
9	25.25871297461089	23.405458150400317	28.545940559336646	22.789888315652142
10-11	24.52231019551557	33.78814429950884	20.68110563932702	21.00843986564857
12-13	25.043356593297517	23.306757606290766	28.869454277153327	22.780431523258386
14-15	23.826100595363872	25.97848671650741	28.14624533988041	22.049167348248304
16-17	25.88937122948814	25.83267246167218	26.846358917980318	21.431597390859363
18-19	25.35693951050215	26.016789771180783	26.869061815520467	21.757208902796602
20-21	24.646250292536426	27.149040834433542	26.60392933016546	21.600779542864576
22-23	26.912451701542366	26.273468658618615	25.475760822639447	21.338318817199564
24-25	23.96121509119824	29.047943946533533	25.832730780342892	21.158110181925334
26-27	23.04796455045803	29.588984643200682	26.338866244617265	21.02418456172402
28-29	24.024712955608518	28.395000951525883	25.500313479539226	22.079972613326373
30-31	26.170946635977817	26.519777154789466	25.791221377153672	21.51805483207905
32-33	23.372662346172547	27.825474166858736	26.77231941986778	22.029544067100936
34-35	22.728267632370976	29.553150771816007	26.318326089133937	21.400255506679073
36-37	21.674906366973826	28.706307426631444	27.41388013556037	22.204906070834358
38-39	21.60462356555008	26.343871775287976	28.868652903776837	23.182851755385105
40-41	25.644675063197397	26.605013459208095	26.486273127173458	21.264038350421043
42-43	26.07742493754589	26.120212282397105	26.714725147543422	21.08763763251358
44-45	26.170382408007104	26.73749240964173	26.73795302712756	20.354172155223598
46-47	25.58798412280093	25.70383905676838	26.407897169815346	22.300279650615344
48-49	22.861306877621963	26.015841237694364	25.96413150039834	25.158720384285328
50-51	24.515568169067585	26.24206434291361	26.28610405975205	22.95626342826676
52-53	22.9445389309477	27.654906387828227	27.916069209315765	21.484485471908307
54-55	22.410263888147668	27.255945196539233	26.8005621798476	23.533228735465507
56-57	21.372108823124062	26.18910854155545	30.380188602454766	22.058594032865724
58-59	22.07818697112916	28.89747121294002	28.14397191125055	20.880369904680276
60-61	21.46871185774892	31.42928338918406	26.321983400986436	20.78002135208058
62-63	20.602697385644287	31.435610119050295	26.733085818736015	21.228606676569406
64-65	21.082307275872918	31.6854695098911	25.882741517967915	21.349481696268064
66-67	22.172939799525572	31.509527344883388	25.27846234529068	21.03907051030036
68-69	21.248317900236437	30.728605218147454	26.76208596540399	21.26099091621212
70-71	21.706862268192566	29.78862162190083	26.625188704627234	21.879327405279373
72-73	22.096401090477254	29.950906966427777	26.102841737196147	21.84985020589882
74-75	22.451559973549998	29.381940453720667	27.105994381979077	21.06050519075026
76-77	22.66082342744489	28.03695903071237	27.236573722712965	22.06564381912978
78-79	23.126807094208118	28.33166949937177	26.159529266896637	22.381994139523474
80-81	23.724417498971896	28.073168486019657	26.410909440906018	21.79150457410243
82-83	23.102954329565637	28.092611593011785	26.69889885155469	22.105535225867886
84-85	22.9374290870793	28.787413164684754	25.89774609709362	22.377411651142324
86-87	22.397672186208396	29.090482628461896	26.524759865276977	21.987085320052728
88-89	22.47966816496045	28.47105206811363	27.328588929722414	21.72069083720351
90-91	23.440287963145202	27.73314766038703	27.086745569284698	21.73981880718307
92-93	22.65429927388392	27.311815848096927	28.083084813533	21.950800064486156
94-95	22.91863751826062	27.87528318210259	26.168013088457492	23.038066211179302
96-97	22.255488445778706	29.403801017668336	26.090584293409847	22.250126243143114
98-99	23.125146439757952	28.967581619128737	26.25832364258265	21.648948298530662
100-101	22.89003352308492	27.891204855991663	26.671270509055034	22.547491111868382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1148.0
1	1443.5
2	2460.5
3	3800.0
4	4352.5
5	3791.0
6	2726.0
7	1792.0
8	1354.5
9	1280.5
10	1331.0
11	1409.5
12	1558.5
13	1779.0
14	2136.0
15	2715.0
16	4172.0
17	6151.0
18	7513.5
19	8156.5
20	9172.5
21	11535.5
22	15213.5
23	19653.5
24	27577.0
25	44606.5
26	72298.5
27	99645.0
28	111880.5
29	115907.0
30	125761.0
31	144922.0
32	172009.5
33	200024.5
34	226019.5
35	272716.5
36	389917.0
37	507053.0
38	568426.0
39	598610.0
40	660062.0
41	772154.5
42	880335.0
43	911363.0
44	965189.0
45	1148237.0
46	1377666.0
47	1502268.5
48	1618280.0
49	1731591.5
50	1541331.5
51	1262020.0
52	1054632.5
53	883632.5
54	698635.5
55	439761.5
56	261761.5
57	164566.5
58	98057.5
59	70338.0
60	57733.0
61	62350.0
62	89862.5
63	92396.5
64	62057.0
65	33030.0
66	16962.5
67	11731.5
68	5220.0
69	3023.0
70	2081.5
71	1532.5
72	1247.5
73	1261.5
74	1172.5
75	947.5
76	866.5
77	898.5
78	858.5
79	714.0
80	767.0
81	684.5
82	589.0
83	613.5
84	571.0
85	541.0
86	474.5
87	353.0
88	222.5
89	160.0
90	130.5
91	96.0
92	71.5
93	57.0
94	48.0
95	48.5
96	41.0
97	38.5
98	35.5
99	32.5
100	81.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04842286043600138
2	0.0524117339467135
3	0.021003281106460568
4	0.013828693093379934
5	0.016206761665570512
6	0.02064881428154914
7	0.0270067825207832
8	0.03979002282676614
9	0.05176113028073683
10-11	0.06711088987595186
12-13	0.08803116292847749
14-15	0.10416164692286453
16-17	0.12227310690710465
18-19	0.1423139432801032
20-21	0.15924758628283384
22-23	0.18370355374080508
24-25	0.19493656255302982
26-27	0.2131130829038676
28-29	0.2435635779324928
30-31	0.2615471606444548
32-33	0.2694890122912044
34-35	0.25947644621791527
36-37	0.22815773181434876
38-39	0.20629520517661554
40-41	0.18761839304125089
42-43	0.16267783802517288
44-45	0.15370623781744636
46-47	0.14555125738356642
48-49	0.13923367144084123
50-51	0.12866921398193043
52-53	0.12600622587326044
54-55	0.1189124024531976
56-57	0.1166150984740248
58-59	0.11721634599982393
60-61	0.11004848836949478
62-63	0.11281018875851988
64-65	0.1110872107741403
66-67	0.10837935344712707
68-69	0.10389018815188805
70-71	0.10483244173709563
72-73	0.09241488556060992
74-75	0.09008841658951404
76-77	0.08567328550454135
78-79	0.07916949230569184
80-81	0.0747902565953937
82-83	0.07635619231557203
84-85	0.08096426103942057
86-87	0.08629921110042925
88-89	0.09472565030528567
90-91	0.10210439326182796
92-93	0.11150449450473222
94-95	0.12422716136595183
96-97	0.13168891237642907
98-99	0.15172974874942757
100-101	0.15509942704700327
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.2286994E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.0762080277757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.99515225547933	18.10142038461104
2	9.702050042729478	4.283689500783722
3	3.497873165033563	2.3165932693816544
4	1.4221729696104568	1.2558474531439991
5	0.6534647090391565	0.721301142777917
6	0.5141047100333075	0.6809689516052762
7	0.35682544797665927	0.5514146973395891
8	0.2296393733048591	0.40556532611568896
9	0.21234818199394917	0.4219058376016557
>10	1.0658676373439389	4.275856715845169
>50	0.10681758376214837	1.6940333427412395
>100	0.1376044917246871	6.849935838068394
>500	0.040592285009822426	6.486933061056607
>1k	0.05344336825383251	25.326189834243262
>5k	0.008204975339389135	12.443018088862079
>10k+	0.003838803365433026	14.185326555822671
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	61660	0.2766636003042851	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	49441	0.22183790241070644	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	43905	0.19699830313590067	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	41952	0.18823534479347012	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	38892	0.17450536398044528	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	38674	0.17352721502056312	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	37435	0.16796791886783832	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	36290	0.16283039336753982	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	36279	0.16278103722736229	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	35548	0.15950109736647303	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	32634	0.14642620714125915	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	32019	0.1436667502131512	No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT	31452	0.14112266553309075	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	27941	0.12536908297278673	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	27772	0.12461079318278634	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	27428	0.12306729207177962	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	27329	0.12262308681018176	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	26825	0.12036167820568355	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	25966	0.11650741235000109	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	25866	0.1160587201665689	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	25837	0.11592859943337357	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	25445	0.11416972607431941	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	25243	0.11326336786378638	No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	24501	0.10993407186271958	No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA	24130	0.10826942386218617	No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC	23576	0.10578366916597186	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	22726	0.1019697856067983	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	22576	0.10129674733165002	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	22439	0.10068203904034793	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	22432	0.10065063058750767	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	22395	0.10048461447963776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.012693501869296506	0.0	0.0
2	0.012913361039178275	0.0	0.0
3	0.014102395325273565	0.0	0.0
4	0.014564548274208715	0.0	8.973843668643693E-6
5	0.01613048399438704	0.0	8.973843668643693E-6
6	0.016579176177819226	0.0	8.973843668643693E-6
7	0.017696419714565363	0.0	8.973843668643693E-6
8	0.01844124873906279	0.0	8.973843668643693E-6
9	0.019132234701548356	0.0	8.973843668643693E-6
10-11	0.021958995457171122	4.486921834321847E-6	8.973843668643693E-6
12-13	0.02446494130163987	6.7303827514827704E-6	1.1217304585804618E-5
14-15	0.026930504849599725	8.973843668643693E-6	1.5704226420126464E-5
16-17	0.0285166317180325	8.973843668643693E-6	3.140845284025293E-5
18-19	0.029306329960873144	8.973843668643693E-6	3.140845284025293E-5
20-21	0.03012294973471972	1.7947687337287387E-5	3.140845284025293E-5
22-23	0.03070176265134724	2.2434609171609236E-5	3.589537467457477E-5
24-25	0.0313523663173239	2.2434609171609236E-5	3.589537467457477E-5
26-27	0.032175716473921964	2.2434609171609236E-5	3.589537467457477E-5
28-29	0.03403554557424837	2.6921531005931082E-5	3.589537467457477E-5
30-31	0.03846189396380688	2.9164991923092005E-5	3.589537467457477E-5
32-33	0.040741250255642375	3.140845284025293E-5	3.81388355917357E-5
34-35	0.04306771922673825	3.140845284025293E-5	4.0382296508896626E-5
36-37	0.04543681395526018	3.140845284025293E-5	4.0382296508896626E-5
38-39	0.04832414815564629	3.140845284025293E-5	4.0382296508896626E-5
40-41	0.052602428124672175	3.365191375741385E-5	4.0382296508896626E-5
42-43	0.057446060244822614	3.589537467457477E-5	4.0382296508896626E-5
44-45	0.06283036644600883	3.589537467457477E-5	4.0382296508896626E-5
46-47	0.06858708715944375	3.589537467457477E-5	4.0382296508896626E-5
48-49	0.07526811377074899	3.589537467457477E-5	4.0382296508896626E-5
50-51	0.08398620289483633	3.589537467457477E-5	4.0382296508896626E-5
52-53	0.09470770261794839	3.589537467457477E-5	4.0382296508896626E-5
54-55	0.10771528901564742	3.589537467457477E-5	4.0382296508896626E-5
56-57	0.12033700013559478	3.589537467457477E-5	4.0382296508896626E-5
58-59	0.13551850016202277	3.589537467457477E-5	4.0382296508896626E-5
60-61	0.15433889379608573	3.589537467457477E-5	4.0382296508896626E-5
62-63	0.17883748701148303	3.589537467457477E-5	4.0382296508896626E-5
64-65	0.2101741491023868	3.589537467457477E-5	4.0382296508896626E-5
66-67	0.24373632442311421	3.589537467457477E-5	4.0382296508896626E-5
68-69	0.28078259454819254	3.589537467457477E-5	4.262575742605755E-5
70-71	0.32864907667673804	3.589537467457477E-5	4.935614017754032E-5
72-73	0.3948895037168314	3.589537467457477E-5	4.935614017754032E-5
74-75	0.4681340157403013	3.81388355917357E-5	4.935614017754032E-5
76-77	0.5404273003348949	4.0382296508896626E-5	4.935614017754032E-5
78-79	0.6264999218826908	4.0382296508896626E-5	4.935614017754032E-5
80-81	0.7327838828331896	4.0382296508896626E-5	4.935614017754032E-5
82-83	0.856544404328372	4.0382296508896626E-5	5.159960109470124E-5
84-85	0.9945890414831179	4.262575742605755E-5	5.3843062011862163E-5
86-87	1.1468617077744985	4.486921834321847E-5	5.3843062011862163E-5
88-89	1.3199290133070436	4.486921834321847E-5	5.3843062011862163E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGGGC	16270	0.0	79.40317	9
GAGCGGC	5945	0.0	74.11275	9
GGGAGAG	30960	0.0	72.61733	5
GAGGGGC	22910	0.0	69.32148	9
AACGGGA	11910	0.0	67.36279	2
GGAGAGC	8665	0.0	66.72518	6
ACGGGAG	7130	0.0	66.57209	3
GAGAGCG	8475	0.0	64.135315	7
AGAGCGG	8315	0.0	64.06022	8
GATTGGG	7360	0.0	63.65518	1
CGGGAAA	24385	0.0	59.487938	4
AAGCGGC	5580	0.0	57.263474	9
GGAGAGG	35785	0.0	54.77153	6
CGGGAGA	35125	0.0	53.798996	4
TTGGGAG	6110	0.0	52.82621	3
GTCCAAT	7670	0.0	51.366165	1
TCCAATC	7790	0.0	50.215164	2
ATTGGGA	9260	0.0	49.626026	2
TAGGGAG	4240	0.0	48.025642	3
GATCCGG	6540	0.0	46.959225	1
>>END_MODULE