##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_1_ATCACG_L002_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22286994
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.722116315910526	33.0	33.0	33.0	33.0	33.0
2	32.68947135715117	33.0	33.0	33.0	33.0	33.0
3	32.685238574569546	33.0	33.0	33.0	33.0	33.0
4	36.55757164918697	37.0	37.0	37.0	37.0	37.0
5	36.47285560358656	37.0	37.0	37.0	37.0	37.0
6	36.43112162187507	37.0	37.0	37.0	37.0	37.0
7	36.41313696230187	37.0	37.0	37.0	37.0	37.0
8	36.3991894106491	37.0	37.0	37.0	37.0	37.0
9	36.412087695630916	37.0	37.0	37.0	37.0	37.0
10-11	36.39811257184347	37.0	37.0	37.0	37.0	37.0
12-13	36.40453539853782	37.0	37.0	37.0	37.0	37.0
14-15	38.41387301939418	40.0	37.0	40.0	37.0	40.0
16-17	38.432358307271045	40.0	37.0	40.0	37.0	40.0
18-19	38.39489107413947	40.0	37.0	40.0	37.0	40.0
20-21	38.424393841538254	40.0	37.0	40.0	37.0	40.0
22-23	38.352139615598226	40.0	37.0	40.0	37.0	40.0
24-25	38.33295672354917	40.0	37.0	40.0	37.0	40.0
26-27	38.280090800939774	40.0	37.0	40.0	37.0	40.0
28-29	38.188773573502104	40.0	37.0	40.0	37.0	40.0
30-31	38.11043281565921	40.0	37.0	40.0	33.0	40.0
32-33	37.98436410939941	40.0	37.0	40.0	33.0	40.0
34-35	37.93456248518755	40.0	37.0	40.0	33.0	40.0
36-37	37.894062833238074	40.0	37.0	40.0	33.0	40.0
38-39	37.84746597051177	40.0	37.0	40.0	33.0	40.0
40-41	37.622580618095014	40.0	37.0	40.0	33.0	40.0
42-43	37.299588719770824	40.0	37.0	40.0	33.0	40.0
44-45	37.51019255894268	40.0	37.0	40.0	33.0	40.0
46-47	37.40934744272826	40.0	37.0	40.0	33.0	40.0
48-49	37.32710582234643	40.0	37.0	40.0	33.0	40.0
50-51	37.11594820728179	38.5	37.0	40.0	33.0	40.0
52-53	36.87743304458196	37.0	37.0	40.0	33.0	40.0
54-55	36.685270095195435	37.0	37.0	40.0	33.0	40.0
56-57	36.47078123231872	37.0	37.0	40.0	33.0	40.0
58-59	36.22064518884871	37.0	37.0	40.0	33.0	40.0
60-61	36.02255559901887	37.0	37.0	38.5	33.0	40.0
62-63	35.952657119214905	37.0	37.0	37.0	33.0	40.0
64-65	35.593398104742164	37.0	37.0	37.0	33.0	40.0
66-67	35.47347841077177	37.0	35.0	37.0	33.0	40.0
68-69	35.194947914465274	37.0	33.0	37.0	33.0	40.0
70-71	34.880629123873774	37.0	33.0	37.0	30.0	37.0
72-73	34.469716059509864	37.0	33.0	37.0	27.0	37.0
74-75	33.695311467306894	37.0	33.0	37.0	27.0	37.0
76-77	33.44825758018331	37.0	33.0	37.0	27.0	37.0
78-79	33.38829823797682	37.0	33.0	37.0	27.0	37.0
80-81	33.39576743727754	37.0	33.0	37.0	27.0	37.0
82-83	33.45382571557205	37.0	33.0	37.0	27.0	37.0
84-85	33.425488919681136	37.0	33.0	37.0	27.0	37.0
86-87	33.3919863979862	37.0	33.0	37.0	27.0	37.0
88-89	33.32975512534351	37.0	33.0	37.0	27.0	37.0
90-91	33.31427219390825	37.0	33.0	37.0	27.0	37.0
92-93	33.24302292628606	37.0	33.0	37.0	27.0	37.0
94-95	33.22370087235632	37.0	33.0	37.0	27.0	37.0
96-97	33.172688048464494	37.0	33.0	37.0	27.0	37.0
98-99	33.085361377133225	37.0	33.0	37.0	27.0	37.0
100-101	32.66929788736875	35.0	33.0	37.0	24.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.06476226008621921
2201	2	0.08491450612712725
2201	3	0.07862811615436271
2201	4	0.12492833048797536
2201	5	0.12192885134342646
2201	6	0.18207545324327157
2201	7	0.18075466663411532
2201	8	0.16073715477519102
2201	9	0.14529028282020562
2201	10-11	0.15445229248748404
2201	12-13	0.14976578036039712
2201	14-15	0.3587904883426063
2201	16-17	0.33580690604399166
2201	18-19	0.3572645528163818
2201	20-21	0.33967660228643126
2201	22-23	0.3323874371747664
2201	24-25	0.0897711409831814
2201	26-27	0.17744021721041037
2201	28-29	0.198077034089728
2201	30-31	0.06875584626762077
2201	32-33	0.18769748806993647
2201	34-35	0.2214176156823342
2201	36-37	0.37919969718829805
2201	38-39	0.39259263572112246
2201	40-41	0.44812899167879294
2201	42-43	0.48008826024924645
2201	44-45	0.40882715449115636
2201	46-47	0.424492419989555
2201	48-49	0.39262987199684574
2201	50-51	0.3792718817307801
2201	52-53	0.3970341732841476
2201	54-55	0.4031631717106734
2201	56-57	0.3954475253187937
2201	58-59	0.3719610650501508
2201	60-61	0.414016110091012
2201	62-63	0.33373919430592736
2201	64-65	0.3721536047033993
2201	66-67	0.37793428971796317
2201	68-69	0.36310299342441255
2201	70-71	0.37835296822783704
2201	72-73	0.3614265603546585
2201	74-75	0.3909485778637247
2201	76-77	0.3117421315926592
2201	78-79	0.3549545905844127
2201	80-81	0.2711642953721878
2201	82-83	0.3301343583212386
2201	84-85	0.37034231036590626
2201	86-87	0.3846436530313255
2201	88-89	0.4595532163447089
2201	90-91	0.4844268372482645
2201	92-93	0.5102292038529299
2201	94-95	0.5467218386037445
2201	96-97	0.4981318326033346
2201	98-99	0.5075763382975325
2201	100-101	0.5523733621516769
1101	1	0.08697404001524944
1101	2	0.09413195255896767
1101	3	0.07759758877237033
1101	4	0.13512201887522224
1101	5	0.14284590386422735
1101	6	0.16196937298246894
1101	7	0.16463660131621793
1101	8	0.15137652293404358
1101	9	0.15817466136378044
1101	10-11	0.16055106443079126
1101	12-13	0.15319985673622227
1101	14-15	-0.03963476769988006
1101	16-17	0.14239723819178352
1101	18-19	0.18690642731832696
1101	20-21	0.18321094035754726
1101	22-23	0.26440256168263687
1101	24-25	0.2912597631235059
1101	26-27	0.08069799204092476
1101	28-29	0.11789061187938188
1101	30-31	0.2940133558545668
1101	32-33	0.29831343889189554
1101	34-35	0.3662869939629445
1101	36-37	0.31140603122960897
1101	38-39	0.31859828575927196
1101	40-41	0.34227382239807724
1101	42-43	0.3075674931471468
1101	44-45	0.19711285580374494
1101	46-47	0.1390170820554104
1101	48-49	0.3333770261012887
1101	50-51	0.30971278635588106
1101	52-53	0.29959148871902386
1101	54-55	0.28497300280614013
1101	56-57	0.3507617337260669
1101	58-59	0.3256852152607692
1101	60-61	0.30061497688242866
1101	62-63	0.2658022980422743
1101	64-65	0.35157081572340587
1101	66-67	0.3441663770985386
1101	68-69	0.21491969434472225
1101	70-71	0.28832337504356076
1101	72-73	0.16746756997603285
1101	74-75	0.3805563028191159
1101	76-77	0.3714294169407495
1101	78-79	0.36583489353036214
1101	80-81	0.3709973055158713
1101	82-83	0.35478558077911515
1101	84-85	0.19679837548548562
1101	86-87	-0.03732688103841042
1101	88-89	0.3324337035950151
1101	90-91	0.4092505717289896
1101	92-93	0.4152679507591728
1101	94-95	0.45776125221715347
1101	96-97	0.09315761171489356
1101	98-99	0.2160934126065186
1101	100-101	0.17968868021767292
2202	1	0.07437971490094952
2202	2	0.060344903706827324
2202	3	0.0759381084339239
2202	4	0.09768482299468673
2202	5	0.09752444648177772
2202	6	0.09410578085869048
2202	7	0.13394833911358006
2202	8	0.14857678912933636
2202	9	0.16252568273137058
2202	10-11	0.15087883996975648
2202	12-13	0.12275466298351034
2202	14-15	0.18834081807550263
2202	16-17	0.11840055444195485
2202	18-19	0.08973067624075526
2202	20-21	0.06433520227918876
2202	22-23	0.1530624331314172
2202	24-25	0.17029282820904257
2202	26-27	0.22582811672927505
2202	28-29	0.17376909141879793
2202	30-31	0.23900358077577977
2202	32-33	0.21898989456997242
2202	34-35	0.29005582765921645
2202	36-37	0.20749909872556316
2202	38-39	0.20194658731246307
2202	40-41	0.11935458285442735
2202	42-43	0.1897470264076162
2202	44-45	0.2381433949352001
2202	46-47	0.22998499418488905
2202	48-49	0.17171675292448185
2202	50-51	0.20650884207354636
2202	52-53	0.2065792278763965
2202	54-55	0.2515031718570455
2202	56-57	0.2863909344561506
2202	58-59	0.17807673389428658
2202	60-61	0.2677969570125498
2202	62-63	0.27430622932788395
2202	64-65	0.27601283454607994
2202	66-67	0.2901573890881508
2202	68-69	0.2896502969194401
2202	70-71	0.2857208429556266
2202	72-73	0.27093750672829486
2202	74-75	0.20016722057462033
2202	76-77	0.09896044870496468
2202	78-79	0.10022527467387476
2202	80-81	0.1527586458304242
2202	82-83	0.20129908689014542
2202	84-85	0.24457701021908207
2202	86-87	0.19568589361630728
2202	88-89	0.2377951832382621
2202	90-91	0.18607559134463258
2202	92-93	0.21040462334271126
2202	94-95	0.21206545350148787
2202	96-97	0.3042134310119522
2202	98-99	0.3747067944044389
2202	100-101	0.4001551057004704
1102	1	0.048994936436855596
1102	2	0.04962861665261897
1102	3	0.04881748512549677
1102	4	0.06107378846297706
1102	5	0.08670450735677804
1102	6	0.09061820786511277
1102	7	0.11134881751243597
1102	8	0.10486790443192007
1102	9	0.12911462827136688
1102	10-11	0.11589963722737906
1102	12-13	0.09748279055531128
1102	14-15	0.2444845103765374
1102	16-17	0.22000102726348558
1102	18-19	0.23437615532518663
1102	20-21	0.23349498448597927
1102	22-23	0.18960240043302434
1102	24-25	0.18569068746368345
1102	26-27	0.2263063112746977
1102	28-29	0.2474936077635661
1102	30-31	0.23747797165766826
1102	32-33	0.293085529196361
1102	34-35	0.24118051004100494
1102	36-37	0.23082426585953897
1102	38-39	0.265624326391098
1102	40-41	0.2392962794363669
1102	42-43	0.24003833230985805
1102	44-45	0.23828904985085586
1102	46-47	0.24818303049172385
1102	48-49	0.29379154346842995
1102	50-51	0.24429564095296286
1102	52-53	0.231151310081124
1102	54-55	0.31398811500950075
1102	56-57	0.2742137079898228
1102	58-59	0.27830811347208595
1102	60-61	0.23246790213273982
1102	62-63	0.24338779829076884
1102	64-65	0.21243615238105917
1102	66-67	0.26372031564317666
1102	68-69	0.20574065107873452
1102	70-71	0.20489147777183803
1102	72-73	0.20263947927072934
1102	74-75	0.20687852689157893
1102	76-77	0.24131500416697094
1102	78-79	0.24012081213215453
1102	80-81	0.23332477151093656
1102	82-83	0.214510069956809
1102	84-85	0.25670392481913495
1102	86-87	0.21285481602168232
1102	88-89	0.26580648668993945
1102	90-91	0.24537753590809075
1102	92-93	0.2804396139589471
1102	94-95	0.3032199020021551
1102	96-97	0.24770637299479858
1102	98-99	0.26198594930338714
1102	100-101	0.28763028828210935
2203	1	0.0042170966636945195
2203	2	0.027568619175738718
2203	3	0.046550373227859154
2203	4	0.07466599082485459
2203	5	0.03403767474367214
2203	6	0.06546451298188316
2203	7	0.0876346313272407
2203	8	0.09783694751865823
2203	9	0.07248142830705717
2203	10-11	0.037278498897897805
2203	12-13	0.062032303648635434
2203	14-15	0.15897255305502256
2203	16-17	0.12744943061765923
2203	18-19	0.1648733116518244
2203	20-21	0.12940475284482744
2203	22-23	0.1345583238633168
2203	24-25	0.16729820675203655
2203	26-27	0.1637352599596653
2203	28-29	0.22676098546698853
2203	30-31	0.18782019754893753
2203	32-33	0.19906126503305188
2203	34-35	0.19005088633674205
2203	36-37	0.20385620714374397
2203	38-39	0.19996197960870887
2203	40-41	0.16475181107451675
2203	42-43	0.14974627612985358
2203	44-45	0.14487900360585826
2203	46-47	0.1196143277434416
2203	48-49	0.08517019952769544
2203	50-51	0.10847119692974871
2203	52-53	0.15241498987274582
2203	54-55	0.14242806101307792
2203	56-57	0.1972865387750744
2203	58-59	0.19246787922075015
2203	60-61	0.15279466741466763
2203	62-63	0.10374799057252915
2203	64-65	0.17310974728633255
2203	66-67	0.15535929797639625
2203	68-69	0.1631079538914122
2203	70-71	0.124238856910587
2203	72-73	0.09541458816459425
2203	74-75	0.1416042058600766
2203	76-77	0.06925060988848486
2203	78-79	0.09976565817417793
2203	80-81	0.13158424680685243
2203	82-83	0.1274367100099738
2203	84-85	0.06549741588676739
2203	86-87	0.10260147563034394
2203	88-89	0.12176718614361448
2203	90-91	0.031074933472694966
2203	92-93	0.13870343366421878
2203	94-95	0.09616658227371033
2203	96-97	0.09085207968293929
2203	98-99	0.11349603769210859
2203	100-101	0.17425678675186163
1103	1	0.017901746139393993
1103	2	0.042129111582838163
1103	3	0.035789769079094924
1103	4	0.06602329731448009
1103	5	0.0768496212187415
1103	6	0.07835436282650932
1103	7	0.058775187256792094
1103	8	0.09045948576417118
1103	9	0.05832050303222758
1103	10-11	0.0651695514353463
1103	12-13	0.08439707892785009
1103	14-15	0.14665163656714242
1103	16-17	0.12401465532954603
1103	18-19	0.12123249863147123
1103	20-21	0.14175200717956926
1103	22-23	0.14540286091673948
1103	24-25	0.14029032852388923
1103	26-27	0.1124416660449441
1103	28-29	0.17393126926454272
1103	30-31	0.18004781881412413
1103	32-33	0.1544641023893405
1103	34-35	0.11001111357758475
1103	36-37	0.08910286985276628
1103	38-39	0.14399287014635576
1103	40-41	0.17546839290711347
1103	42-43	0.15414288379884056
1103	44-45	0.07975161032568678
1103	46-47	0.11622126808183708
1103	48-49	0.16990507676413813
1103	50-51	0.153607493005957
1103	52-53	0.12756158383596272
1103	54-55	0.14777001056997108
1103	56-57	0.12293263432808743
1103	58-59	0.14616682835632133
1103	60-61	0.13840800388882712
1103	62-63	0.05349776808888862
1103	64-65	0.1006696439037782
1103	66-67	0.1057330511586585
1103	68-69	0.10020019485570941
1103	70-71	0.15161204786165428
1103	72-73	0.06554855212808519
1103	74-75	0.06174281498169876
1103	76-77	0.1106574305314254
1103	78-79	0.07197407793914579
1103	80-81	0.04940215090605449
1103	82-83	0.10356638096117621
1103	84-85	0.0989464682263872
1103	86-87	0.125794787862894
1103	88-89	0.13669519400108499
1103	90-91	0.12392517299342387
1103	92-93	0.10967028918979338
1103	94-95	0.11933316986555553
1103	96-97	0.10889183729720742
1103	98-99	0.1321626160862408
1103	100-101	0.17181773960707858
2204	1	0.03870015525518511
2204	2	0.03543743723373183
2204	3	0.04959171781020899
2204	4	0.05230192104422571
2204	5	0.07023946123943148
2204	6	0.059753034813390116
2204	7	0.0523850415081597
2204	8	0.04347067054040821
2204	9	0.08181422383550796
2204	10-11	0.07055727059105976
2204	12-13	0.0808602893381476
2204	14-15	0.1331942302782494
2204	16-17	0.10289201368311751
2204	18-19	0.11644832725428245
2204	20-21	0.08344150532655448
2204	22-23	0.024421858827672338
2204	24-25	0.08299954121753217
2204	26-27	0.07165824505521812
2204	28-29	0.10535580450116555
2204	30-31	0.09583939320314983
2204	32-33	0.11172262931440002
2204	34-35	0.1459946843308373
2204	36-37	0.1541454696470339
2204	38-39	0.12813958109843782
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1213	40-41	-0.590064966949619
1213	42-43	-0.6053015670121624
1213	44-45	-0.6816999967874935
1213	46-47	-0.5808394632692
1213	48-49	-0.7393083510470575
1213	50-51	-0.6245446041819349
1213	52-53	-0.6592374938682681
1213	54-55	-0.6498412161725113
1213	56-57	-0.665423921419638
1213	58-59	-0.7225208978833066
1213	60-61	-0.7026507052630677
1213	62-63	-0.6676287016836326
1213	64-65	-0.6043655417769429
1213	66-67	-0.6049609470932609
1213	68-69	-0.6429795185761122
1213	70-71	-0.6937937678768549
1213	72-73	-0.7116404069995852
1213	74-75	-0.5387512840703508
1213	76-77	-0.5652242678647994
1213	78-79	-0.6543995539359599
1213	80-81	-0.6089946763416378
1213	82-83	-0.5941129543142907
1213	84-85	-0.5226067779663097
1213	86-87	-0.6212812007380748
1213	88-89	-0.5356460191281869
1213	90-91	-0.6029957876206069
1213	92-93	-0.793141760583083
1213	94-95	-0.6979556998125886
1213	96-97	-0.8058355101243322
1213	98-99	-0.7682633615863637
1213	100-101	-0.7653114405570136
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	14.0
12	60.0
13	214.0
14	824.0
15	2116.0
16	4197.0
17	7337.0
18	12646.0
19	20178.0
20	31136.0
21	44767.0
22	61702.0
23	82457.0
24	109332.0
25	144404.0
26	190789.0
27	257997.0
28	369187.0
29	629204.0
30	530221.0
31	423732.0
32	553383.0
33	751884.0
34	1040810.0
35	1446704.0
36	2535600.0
37	7890171.0
38	5141652.0
39	4273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.19213092747385	11.476690421971751	15.835150207197895	35.49602844335651
2	22.210451530610182	23.308778204902826	35.325715078489274	19.155055185997718
3	23.400059494593965	22.54337055365877	28.77395267792097	25.28261727382629
4	25.415186094634386	31.43489427062259	19.215175451655796	23.934744183087233
5	27.673376628562952	32.815682018324566	21.274536189525023	18.23640516358746
6	21.519254682798408	34.401952098160926	25.8239177522101	18.254875466830566
7	15.056857824792344	20.84267622632285	41.86413834005609	22.236327608828717
8	18.81305751686387	24.295129257898125	28.681378027023296	28.210435198214707
9	27.222661790997925	21.065546120755453	28.771058133725884	22.940733954520738
10-11	24.38679034059057	33.61432232628591	20.94157695739497	21.05731037572855
12-13	21.045312346743575	25.255693971111583	29.008826852109355	24.69016683003549
14-15	21.55161884998937	27.932993116972167	27.60281175648901	22.912576276549455
16-17	24.462733736097388	25.60392173121238	26.31584815789873	23.617496374791504
18-19	21.46700447803773	25.908626349520265	28.45549292111803	24.16887625132398
20-21	24.290833030241764	25.893009618075908	28.347337913762622	21.468819437919713
22-23	21.880850293400155	31.157080556573547	25.522153111008585	21.439916039017717
24-25	21.394116676300087	26.57149950325288	28.331644904647078	23.70273891579995
26-27	22.042389386383825	25.509353123171298	26.35636954898449	26.09188794146039
28-29	24.090473327810813	28.220505281079188	25.548451688393758	22.14056970271624
30-31	21.63227643413658	26.085353225970913	28.224199136962184	24.058171202930325
32-33	20.972164558550638	28.364162414860488	26.2207414854432	24.442931541145676
34-35	20.432103194190727	29.42226925115194	28.693752332919235	21.4518752217381
36-37	21.21035811697163	29.279990297442787	25.629501858220976	23.880149727364604
38-39	23.787716728420172	25.97655385917006	26.366669278055177	23.86906013435459
40-41	21.607395119181138	28.792406719709447	28.92508546269928	20.675112698410132
42-43	21.55750300483634	26.107422548695737	29.022475558888345	23.31259888757958
44-45	23.8543407872771	26.63156592584895	26.68024005390767	22.833853232966277
46-47	21.89831889690964	27.972874575636624	28.58893115484719	21.53987537260654
48-49	23.886549072939086	25.79230745812006	28.525150466978168	21.79599300196269
50-51	21.053984487535946	25.91132734862423	26.13747978460651	26.897208379233316
52-53	24.435054019992698	25.91225221556846	28.42903702906025	21.223656735378594
54-55	22.15480397366311	25.37795532528671	28.251941126645956	24.215299574404217
56-57	21.221604457810887	25.630175860870956	28.77114467617951	24.37707500513865
58-59	24.30584995389377	25.707923231819734	29.11589746986971	20.87032934441678
60-61	21.33900558863299	26.45046634770515	28.921332798988274	23.289195264673584
62-63	22.943268793414422	26.44522919395615	29.338175633276386	21.27332637935304
64-65	20.9341869260085	31.68273676280365	25.989118450152187	21.393957861035666
66-67	21.939494781105907	31.738642520163197	25.25151542134281	21.07034727738809
68-69	21.011858995794487	31.22712819575957	26.669264941394	21.09174786705194
70-71	21.194431734523064	30.95479808537171	26.342720527917102	21.508049652188124
72-73	21.321895394959206	31.456942773775587	25.71287868530417	21.508283145961034
74-75	21.734934783199336	30.36664677601492	26.806705470226877	21.091712970558866
76-77	22.18261323908842	28.656265328201968	27.072022915571498	22.08909851713812
78-79	22.74615785471805	28.700355697773645	26.100467189287624	22.45301925822068
80-81	23.228988359444877	28.399168266684566	26.35042733374247	22.021416040128088
82-83	22.680476652317534	28.315904106095612	26.71404877192011	22.289570469666746
84-85	22.412067821479795	29.032311621178813	25.92472867986608	22.630891877475314
86-87	21.951527445601904	29.265137282138653	26.44033920624142	22.342996066018024
88-89	22.194624331167752	28.581776685518513	27.30150949744988	21.922089485863854
90-91	22.858159923753913	27.884493244269486	27.180165481936086	22.077181350040515
92-93	22.244701998077428	27.331824018266865	28.128627765695256	22.294846217960448
94-95	22.526303039566812	27.896721474468674	26.29050530768431	23.2864701782802
96-97	21.742911271024106	29.42575136645737	26.11429825273841	22.717039109780117
98-99	22.69258206368507	28.705631302737476	26.45581155122742	22.14597508235003
100-101	22.456965107067944	27.659219764228727	26.85174259356925	23.03207253513408
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	860.0
1	1029.5
2	974.5
3	733.0
4	647.5
5	519.0
6	453.5
7	455.5
8	497.0
9	520.5
10	545.5
11	599.5
12	649.5
13	786.0
14	1083.0
15	1588.0
16	3075.5
17	5031.0
18	6499.5
19	6955.0
20	7496.0
21	9785.0
22	13270.5
23	17910.5
24	26136.0
25	43430.5
26	70884.0
27	98233.0
28	109511.0
29	111695.5
30	121512.0
31	141074.5
32	169428.0
33	197954.5
34	223665.0
35	271858.5
36	405275.0
37	532852.0
38	598391.0
39	645901.0
40	732166.0
41	871316.5
42	993262.5
43	1014907.5
44	1041510.0
45	1192531.0
46	1379522.5
47	1467680.5
48	1557743.5
49	1649682.0
50	1463402.5
51	1197900.5
52	995695.0
53	837034.5
54	666616.0
55	411759.0
56	238643.5
57	149203.0
58	86724.5
59	62134.5
60	51230.0
61	57605.0
62	92523.5
63	96102.5
64	59840.0
65	29262.0
66	14861.0
67	9765.0
68	2609.0
69	1720.5
70	1319.0
71	1085.0
72	858.5
73	864.0
74	879.0
75	698.5
76	653.0
77	716.5
78	636.0
79	489.5
80	634.0
81	555.5
82	482.5
83	539.5
84	486.0
85	517.5
86	473.5
87	330.5
88	196.5
89	109.0
90	55.0
91	34.0
92	16.0
93	19.5
94	23.0
95	17.0
96	10.0
97	5.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02096738573178599
2	0.0
3	0.026822818725576
4	0.0
5	0.00281778691195412
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.002025845208196314
24-25	0.0
26-27	0.0
28-29	3.23058372071173E-4
30-31	9.646881943791971E-5
32-33	8.570020703554728E-4
34-35	1.3460765502965543E-5
36-37	3.8587527775167885E-4
38-39	0.0
40-41	3.4997990307710403E-4
42-43	2.9164991923092005E-5
44-45	0.0
46-47	3.589537467457478E-4
48-49	0.0020617405828708885
50-51	0.0686072783076982
52-53	4.778571753552768E-4
54-55	6.730382751482771E-6
56-57	4.4869218343218475E-6
58-59	0.028637778607559186
60-61	0.019132234701548356
62-63	0.0021402617149715213
64-65	0.007437072940388462
66-67	1.8172033429003482E-4
68-69	0.0018418814129891182
70-71	7.246378762429783E-4
72-73	0.0041571330794991914
74-75	0.004399426858552572
76-77	0.002977072637072545
78-79	4.3074449609489736E-4
80-81	0.01637502123435758
82-83	0.011176922289295722
84-85	0.005749990330683447
86-87	0.010416389038378167
88-89	0.0030039941680784768
90-91	9.467405070419097E-4
92-93	0.010194286407579236
94-95	0.01824158071743547
96-97	0.005671469198582815
98-99	0.00801588585701598
100-101	0.01299636909311323
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.2286994E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.687461215172718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10677379654634	13.034037583721359
2	12.605247004563196	4.206991409926409
3	3.621090310439106	1.8128041233627097
4	1.789545452457385	1.1945188132268532
5	0.86269878226119	0.7198126234680172
6	0.5249710297766812	0.525626021909259
7	0.3576252763240945	0.4177500549756628
8	0.22127208770166026	0.29539755052174016
9	0.21114335969948864	0.31711019632438314
>10	1.2454766635080177	3.843821133047369
>50	0.12649423863500825	1.5302281116869945
>100	0.1829118985481698	7.035983076298871
>500	0.055550304239080285	6.572115397067972
>1k	0.07231449198661075	26.044003188828306
>5k	0.011649584660266308	13.337805723587811
>10k+	0.005235718653334227	19.111994992046075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	938939	4.2129459001963205	TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC	71790	0.32211611848596544	TruSeq Adapter, Index 1 (98% over 50bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	65635	0.29449911459571443	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	50872	0.228258687555621	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	43097	0.19337287029376865	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	40037	0.17964288948074378	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	38825	0.1742047402175457	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	38376	0.17219011231393522	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	34655	0.15549427616842362	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	34426	0.1544667710683639	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	33931	0.1522457447603746	No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT	32821	0.14726526152427735	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	32068	0.143886609383033	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	31326	0.14055731338196617	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	30492	0.13681522057214177	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	29849	0.1339301298326728	No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA	29122	0.13066813765912083	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	28818	0.12930411342148698	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	28813	0.12928167881231536	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	28747	0.12898554197125014	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	28536	0.1280388014642082	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	28054	0.12587610514006511	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	27652	0.12407236256266772	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	26466	0.11875087326716201	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	26256	0.11780861968195443	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	25569	0.11472610438177532	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	24963	0.11200702975017628	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	24707	0.11085837776058988	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	24468	0.10978600344218696	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	24202	0.10859248223425734	No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG	23157	0.10390364891739101	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	22900	0.10275051000597028	No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT	22779	0.10220759246401735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.012213401233024068	0.0	0.0
2	0.012446721168408803	0.0	0.0
3	0.013644729298172738	0.0	0.0
4	0.014124829934445174	0.0	1.3460765502965541E-5
5	0.015731147951132396	0.0	1.3460765502965541E-5
6	0.016188813978233223	0.0	1.3460765502965541E-5
7	0.017306057514979364	0.0	1.3460765502965541E-5
8	0.018055373461311114	4.486921834321847E-6	1.3460765502965541E-5
9	0.018759820189299642	4.486921834321847E-6	1.7947687337287387E-5
10-11	0.02157312017941944	4.486921834321847E-6	2.2434609171609236E-5
12-13	0.024065605258385227	4.486921834321847E-6	2.6921531005931082E-5
14-15	0.026510977658090636	4.486921834321847E-6	2.6921531005931082E-5
16-17	0.028063452612765993	4.486921834321847E-6	2.6921531005931082E-5
18-19	0.028877828925695407	4.486921834321847E-6	2.6921531005931082E-5
20-21	0.029725857152382237	4.486921834321847E-6	2.6921531005931082E-5
22-23	0.030324861217264205	4.486921834321847E-6	2.9164991923092005E-5
24-25	0.03097770834415803	4.486921834321847E-6	3.589537467457477E-5
26-27	0.03178311081341881	4.486921834321847E-6	3.589537467457477E-5
28-29	0.0336205053045736	4.486921834321847E-6	3.589537467457477E-5
30-31	0.038046853694132104	4.486921834321847E-6	3.81388355917357E-5
32-33	0.040348644595139205	4.486921834321847E-6	4.0382296508896626E-5
34-35	0.04267735702715225	4.486921834321847E-6	4.0382296508896626E-5
36-37	0.04507786020851444	4.486921834321847E-6	4.0382296508896626E-5
38-39	0.04802128093182957	4.486921834321847E-6	4.0382296508896626E-5
40-41	0.05231750858819274	4.486921834321847E-6	4.0382296508896626E-5
42-43	0.05715216686467453	4.486921834321847E-6	4.0382296508896626E-5
44-45	0.06251852537852345	4.486921834321847E-6	4.0382296508896626E-5
46-47	0.0682460811000353	4.486921834321847E-6	4.0382296508896626E-5
48-49	0.07493159463317485	4.486921834321847E-6	4.0382296508896626E-5
50-51	0.08371025720202555	4.486921834321847E-6	4.0382296508896626E-5
52-53	0.09447438268256365	4.486921834321847E-6	4.0382296508896626E-5
54-55	0.10748869946301418	4.486921834321847E-6	4.0382296508896626E-5
56-57	0.1200947063565414	4.486921834321847E-6	4.0382296508896626E-5
58-59	0.13522685024279182	6.7303827514827704E-6	4.0382296508896626E-5
60-61	0.1540472438768548	1.1217304585804618E-5	4.0382296508896626E-5
62-63	0.17894517313550673	1.5704226420126464E-5	4.0382296508896626E-5
64-65	0.21039400827226856	1.7947687337287387E-5	4.0382296508896626E-5
66-67	0.24387093207814386	1.7947687337287387E-5	4.0382296508896626E-5
68-69	0.28070407341609194	1.7947687337287387E-5	5.3843062011862163E-5
70-71	0.3281958975714715	1.7947687337287387E-5	5.832998384618401E-5
72-73	0.3939562239752925	1.7947687337287387E-5	5.832998384618401E-5
74-75	0.46688889493127694	1.7947687337287387E-5	5.832998384618401E-5
76-77	0.5387245134987697	1.7947687337287387E-5	5.832998384618401E-5
78-79	0.62445388552624	1.7947687337287387E-5	5.832998384618401E-5
80-81	0.7303205627461469	1.7947687337287387E-5	6.281690568050586E-5
82-83	0.8539846154218913	1.7947687337287387E-5	6.281690568050586E-5
84-85	0.9921122606305722	2.2434609171609236E-5	6.506036659766678E-5
86-87	1.144571134178077	2.467807008877016E-5	6.73038275148277E-5
88-89	1.3174208240016578	2.9164991923092005E-5	6.73038275148277E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGAGCA	147405	0.0	70.76664	7
AGAGCAC	152960	0.0	68.15938	8
CGGAAGA	153580	0.0	67.957016	4
TCGGAAG	154405	0.0	67.63016	3
GAGCACA	157165	0.0	66.550285	9
GATCGGA	159450	0.0	65.51251	1
GAAGAGC	170235	0.0	61.306904	6
GGAAGAG	173650	0.0	60.13281	5
ATCGGAA	183830	0.0	56.822937	2
GTCCAAT	7070	0.0	47.980152	1
TCCAATC	7290	0.0	47.109215	2
GCCGTCT	113980	0.0	45.347126	48-49
GTATGCC	114450	0.0	45.336494	44-45
ATGCCGT	114405	0.0	45.259693	46-47
TGCCGTC	113735	0.0	45.246265	48-49
TATGCCG	114205	0.0	45.220318	46-47
CGTATGC	114765	0.0	45.058846	44-45
CTCGTAT	105895	0.0	44.913914	42-43
TCGTATG	115985	0.0	44.77773	42-43
CCGTCTT	114965	0.0	44.772522	50-51
>>END_MODULE