##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_1_ATCACG_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22524992
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301188741820642	33.0	33.0	33.0	27.0	33.0
2	31.056621240975357	33.0	33.0	33.0	27.0	33.0
3	30.981139393967375	33.0	33.0	33.0	27.0	33.0
4	34.5686782929823	37.0	37.0	37.0	33.0	37.0
5	34.59240589297435	37.0	37.0	37.0	33.0	37.0
6	34.87020887732169	37.0	37.0	37.0	33.0	37.0
7	34.504957782004986	37.0	37.0	37.0	33.0	37.0
8	34.800765256653584	37.0	37.0	37.0	33.0	37.0
9	34.454070083576504	37.0	37.0	37.0	27.0	37.0
10-11	34.53990318842288	37.0	37.0	37.0	30.0	37.0
12-13	34.45746648877833	37.0	37.0	37.0	27.0	37.0
14-15	36.200324688239625	40.0	37.0	40.0	27.0	40.0
16-17	36.18807622661975	40.0	37.0	40.0	27.0	40.0
18-19	36.194242133360135	40.0	37.0	40.0	27.0	40.0
20-21	36.11309014005421	40.0	37.0	40.0	27.0	40.0
22-23	36.422468807092145	40.0	37.0	40.0	27.0	40.0
24-25	36.03388598317815	40.0	37.0	40.0	27.0	40.0
26-27	35.99496188500311	40.0	37.0	40.0	27.0	40.0
28-29	35.51719858546454	40.0	37.0	40.0	27.0	40.0
30-31	35.40947717095749	40.0	37.0	40.0	27.0	40.0
32-33	35.31974914796862	38.5	37.0	40.0	27.0	40.0
34-35	35.466262562934546	40.0	37.0	40.0	27.0	40.0
36-37	35.497895759519025	40.0	37.0	40.0	27.0	40.0
38-39	35.514452280382606	40.0	37.0	40.0	27.0	40.0
40-41	35.50735589606425	40.0	37.0	40.0	27.0	40.0
42-43	35.308107123856026	38.5	37.0	40.0	22.0	40.0
44-45	35.29199650770131	37.0	37.0	40.0	22.0	40.0
46-47	34.9340948489571	37.0	37.0	40.0	22.0	40.0
48-49	34.77251723330246	37.0	37.0	40.0	22.0	40.0
50-51	34.52646482626942	37.0	33.0	40.0	22.0	40.0
52-53	34.394917476552266	37.0	33.0	40.0	22.0	40.0
54-55	34.19581372104372	37.0	33.0	40.0	22.0	40.0
56-57	34.1721842120965	37.0	33.0	40.0	22.0	40.0
58-59	33.81225806872651	37.0	33.0	40.0	22.0	40.0
60-61	34.156828002425044	37.0	33.0	37.0	24.5	40.0
62-63	34.11014549971871	37.0	33.0	37.0	27.0	40.0
64-65	33.93059145148642	37.0	33.0	37.0	27.0	40.0
66-67	33.84239408386915	37.0	33.0	37.0	24.5	40.0
68-69	33.495467878523556	37.0	33.0	37.0	22.0	38.5
70-71	33.06308233095044	37.0	33.0	37.0	22.0	37.0
72-73	32.88038659458792	37.0	33.0	37.0	22.0	37.0
74-75	32.76660116016912	37.0	33.0	37.0	22.0	37.0
76-77	32.65714014460028	37.0	33.0	37.0	22.0	37.0
78-79	32.55997604793822	37.0	33.0	37.0	22.0	37.0
80-81	32.45618131629081	37.0	33.0	37.0	22.0	37.0
82-83	32.37030929911096	37.0	33.0	37.0	22.0	37.0
84-85	32.29778523339764	37.0	33.0	37.0	22.0	37.0
86-87	32.20710275945936	37.0	33.0	37.0	22.0	37.0
88-89	32.115471872309655	37.0	33.0	37.0	22.0	37.0
90-91	32.03597457437499	37.0	33.0	37.0	18.5	37.0
92-93	31.961283959612505	37.0	33.0	37.0	15.0	37.0
94-95	31.920189272431262	37.0	33.0	37.0	15.0	37.0
96-97	31.827787774575015	37.0	33.0	37.0	15.0	37.0
98-99	31.723603431246502	37.0	33.0	37.0	15.0	37.0
100-101	31.26389103712001	35.0	30.0	37.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2201	1	0.23535201251075577
2201	2	0.26771652175630933
2201	3	0.2216962858355167
2201	4	0.3157386477389039
2201	5	0.35119211206244216
2201	6	0.4162218122508321
2201	7	0.27793108005012357
2201	8	0.4107391309523436
2201	9	0.43252610309833983
2201	10-11	0.3243683546521581
2201	12-13	0.2638290611618146
2201	14-15	0.5041236719399862
2201	16-17	0.6202276123012922
2201	18-19	0.49544810290346675
2201	20-21	0.4760899177469611
2201	22-23	0.51573150301261
2201	24-25	0.5856525190801776
2201	26-27	0.6466059158387765
2201	28-29	0.7935729540096972
2201	30-31	1.0055172662152856
2201	32-33	0.9470865327150051
2201	34-35	0.8011461476121156
2201	36-37	0.8165098710747714
2201	38-39	0.7404579183163804
2201	40-41	0.7038607470920084
2201	42-43	0.7075767317717734
2201	44-45	0.7737839152744144
2201	46-47	0.7209891644028588
2201	48-49	0.7846906231918069
2201	50-51	0.7306254898529048
2201	52-53	0.746910774092143
2201	54-55	0.732979853855575
2201	56-57	0.7477622275131779
2201	58-59	0.7428057102602494
2201	60-61	0.696969783406459
2201	62-63	0.713123158312996
2201	64-65	0.7453350430424166
2201	66-67	0.6725807427353203
2201	68-69	0.7259619123128971
2201	70-71	0.7113337856996012
2201	72-73	0.6468033879230788
2201	74-75	0.6351484209966998
2201	76-77	0.6727242979302801
2201	78-79	0.6559352327948247
2201	80-81	0.6358615867563557
2201	82-83	0.6074795894977782
2201	84-85	0.6856768639519331
2201	86-87	0.7074223172593648
2201	88-89	0.6593303064888048
2201	90-91	0.724925410874036
2201	92-93	0.7481405741563805
2201	94-95	0.6859787252957723
2201	96-97	0.7787763683040403
2201	98-99	0.7733158442481276
2201	100-101	0.7509201929918738
1101	1	0.3064418235114985
1101	2	0.2761150538686543
1101	3	0.27179124471488336
1101	4	0.3071043311107715
1101	5	0.29841399596219986
1101	6	0.260056047269245
1101	7	0.3058148572234245
1101	8	0.3335327555615919
1101	9	0.27145931212391616
1101	10-11	0.3033772989547785
1101	12-13	0.3455426521466123
1101	14-15	0.481205636495865
1101	16-17	0.4739362993441887
1101	18-19	0.5140006846285274
1101	20-21	0.5210069786011928
1101	22-23	0.6527641662493835
1101	24-25	0.6341353153434426
1101	26-27	0.6094651266182538
1101	28-29	0.845967952440354
1101	30-31	0.8753916923772991
1101	32-33	0.791633914815236
1101	34-35	0.6063058125932059
1101	36-37	0.6550737018699948
1101	38-39	0.6451893570922067
1101	40-41	0.6330005591278933
1101	42-43	0.5785917089813353
1101	44-45	0.6695135839893567
1101	46-47	0.6665973224531072
1101	48-49	0.682502140230369
1101	50-51	0.6670117894660095
1101	52-53	0.6723487345624761
1101	54-55	0.7095557667903734
1101	56-57	0.673828320689978
1101	58-59	0.6398315940408565
1101	60-61	0.6506168565083996
1101	62-63	0.6607428925596608
1101	64-65	0.6793311364391741
1101	66-67	0.6622341899672151
1101	68-69	0.6136583467876235
1101	70-71	0.6126479515765695
1101	72-73	0.6121828308456472
1101	74-75	0.6398045414853399
1101	76-77	0.6181130386248768
1101	78-79	0.5919647291674295
1101	80-81	0.6126513287532092
1101	82-83	0.5799897716026479
1101	84-85	0.622143955083331
1101	86-87	0.5320810645756566
1101	88-89	0.5884059855447461
1101	90-91	0.6419771451755878
1101	92-93	0.6229111005308496
1101	94-95	0.640349620130511
1101	96-97	0.6734326529002779
1101	98-99	0.6087721318542911
1101	100-101	0.6519038190130502
2202	1	0.22732102252543385
2202	2	0.24185591318629562
2202	3	0.2510010220420753
2202	4	0.26606406405022653
2202	5	0.2947213604622192
2202	6	0.4162028296378324
2202	7	0.31449322241815736
2202	8	0.33317828113293757
2202	9	0.3091671732295751
2202	10-11	0.34845109692496834
2202	12-13	0.3551161640783391
2202	14-15	0.5147610955257278
2202	16-17	0.5255979862359226
2202	18-19	0.5477800716740404
2202	20-21	0.5466981779663556
2202	22-23	0.5894546757624965
2202	24-25	0.5943912176452031
2202	26-27	0.6009557184003995
2202	28-29	0.7982340551064269
2202	30-31	0.8944601033873667
2202	32-33	0.7935506548183753
2202	34-35	0.6177104884931026
2202	36-37	0.6055472232759698
2202	38-39	0.5864682661167251
2202	40-41	0.5939951749342995
2202	42-43	0.5834767343244067
2202	44-45	0.6438442856434179
2202	46-47	0.5821677942654517
2202	48-49	0.6080823180007684
2202	50-51	0.6165497693292039
2202	52-53	0.6078794302287776
2202	54-55	0.6107300755855078
2202	56-57	0.6895739780431072
2202	58-59	0.6953287492552178
2202	60-61	0.6870783651508319
2202	62-63	0.6275536494145939
2202	64-65	0.563609764862548
2202	66-67	0.5698038194505415
2202	68-69	0.6203091365853695
2202	70-71	0.5662375537871114
2202	72-73	0.6125795213452605
2202	74-75	0.6475107320385831
2202	76-77	0.639754399219477
2202	78-79	0.530973597192741
2202	80-81	0.5543798172881438
2202	82-83	0.5358576298591444
2202	84-85	0.48049555271159505
2202	86-87	0.5520223984058035
2202	88-89	0.5246622618455561
2202	90-91	0.5818045883682785
2202	92-93	0.5460545762600972
2202	94-95	0.6260554112929277
2202	96-97	0.5756788078234614
2202	98-99	0.6518698102614984
2202	100-101	0.6756329830621546
1102	1	0.2211763738554673
1102	2	0.22744593119385925
1102	3	0.2046776920136928
1102	4	0.2487298244468903
1102	5	0.20559935052558842
1102	6	0.33462192349863784
1102	7	0.27360915091870197
1102	8	0.24024873605531383
1102	9	0.2598239240536486
1102	10-11	0.2573394520870451
1102	12-13	0.27641014659985785
1102	14-15	0.37911178930125544
1102	16-17	0.39800259023628115
1102	18-19	0.44772708264626004
1102	20-21	0.45440884758895095
1102	22-23	0.5128454643349727
1102	24-25	0.49375250233790524
1102	26-27	0.5799476818816558
1102	28-29	0.7681586805835536
1102	30-31	0.7953803420901764
1102	32-33	0.6979390121138422
1102	34-35	0.5557523055320956
1102	36-37	0.5252845490285551
1102	38-39	0.5021525528706832
1102	40-41	0.529431917131447
1102	42-43	0.4620627461974891
1102	44-45	0.5716131477624131
1102	46-47	0.471067405842426
1102	48-49	0.5026706778616514
1102	50-51	0.4471543245598397
1102	52-53	0.4727279632533552
1102	54-55	0.4045421598659402
1102	56-57	0.45464713377207744
1102	58-59	0.443717179393623
1102	60-61	0.5136695698188873
1102	62-63	0.5690928606598078
1102	64-65	0.4782628478050128
1102	66-67	0.5208971330395045
1102	68-69	0.4059115095371979
1102	70-71	0.4428895694721291
1102	72-73	0.46962459024783954
1102	74-75	0.44628428880901083
1102	76-77	0.4800061736426713
1102	78-79	0.40384204662289136
1102	80-81	0.4568488345936359
1102	82-83	0.505261931880959
1102	84-85	0.4586740620879084
1102	86-87	0.43778387152712384
1102	88-89	0.4824539796082803
1102	90-91	0.47817352080760145
1102	92-93	0.48107494600130707
1102	94-95	0.5447383100317076
1102	96-97	0.5282370122574811
1102	98-99	0.5096294482382326
1102	100-101	0.5854305474942869
2203	1	0.12830150811885233
2203	2	0.1260768972848254
2203	3	0.0579547520882393
2203	4	0.09058927310010745
2203	5	0.1270597845227428
2203	6	0.15095135058536613
2203	7	0.12462198995039131
2203	8	0.11191767765796357
2203	9	0.09332564656779851
2203	10-11	0.09384039329931682
2203	12-13	0.0376639235073668
2203	14-15	0.10607668117735614
2203	16-17	0.15579603154996846
2203	18-19	0.24119471492194577
2203	20-21	0.14381057574524903
2203	22-23	0.3153656407408363
2203	24-25	0.1715089680621631
2203	26-27	0.26267936828615746
2203	28-29	0.4294337415837006
2203	30-31	0.47594867776604133
2203	32-33	0.34574100489804493
2203	34-35	0.21380408673405782
2203	36-37	0.1517643450767352
2203	38-39	0.1271475866611098
2203	40-41	0.19101551065595856
2203	42-43	0.19845733805455978
2203	44-45	0.2639203991291197
2203	46-47	0.17123706967007735
2203	48-49	0.1014870927618503
2203	50-51	0.17781435788771915
2203	52-53	0.1902493208696967
2203	54-55	0.14686327784153264
2203	56-57	0.22605224445789673
2203	58-59	0.11697906289153792
2203	60-61	0.22683511391846167
2203	62-63	0.2076819362076705
2203	64-65	0.2534242346772544
2203	66-67	0.22250929389792873
2203	68-69	0.24957028216983446
2203	70-71	0.14771925428411237
2203	72-73	0.14232349665407895
2203	74-75	0.08750218973134594
2203	76-77	0.11915722368636494
2203	78-79	0.08514681482851216
2203	80-81	0.1557143600710802
2203	82-83	0.1378118168726843
2203	84-85	0.1794419771624618
2203	86-87	0.16388462386560576
2203	88-89	0.16027294546830007
2203	90-91	0.1635918239728369
2203	92-93	0.2036809648086475
2203	94-95	0.20573433335153268
2203	96-97	0.2688869741468558
2203	98-99	0.24542866980392475
2203	100-101	0.1980268944120951
1103	1	0.1933907979790277
1103	2	0.15462013911742645
1103	3	0.14652634505570816
1103	4	0.15488339855189537
1103	5	0.1883142015829904
1103	6	0.18697586824912804
1103	7	0.19431983965770883
1103	8	0.24642606705753423
1103	9	0.17806765225512322
1103	10-11	0.19247124277141126
1103	12-13	0.13893769831422276
1103	14-15	0.22322254185563395
1103	16-17	0.22443767826290184
1103	18-19	0.24268834602334266
1103	20-21	0.2444220328822695
1103	22-23	0.30723164666358116
1103	24-25	0.36815537483942506
1103	26-27	0.37238623729147946
1103	28-29	0.6045814831837859
1103	30-31	0.6353052834857849
1103	32-33	0.5642469412828035
1103	34-35	0.3309832355667339
1103	36-37	0.3340281078136442
1103	38-39	0.3185945726906141
1103	40-41	0.32036757972858965
1103	42-43	0.3791464468497807
1103	44-45	0.409352056803705
1103	46-47	0.3052723124521748
1103	48-49	0.3274582210936856
1103	50-51	0.3898291283016988
1103	52-53	0.36806205761438093
1103	54-55	0.37350354095249827
1103	56-57	0.37327233876055743
1103	58-59	0.405088365270835
1103	60-61	0.4273235419373336
1103	62-63	0.42588335002997013
1103	64-65	0.3598220812943538
1103	66-67	0.3909731464160018
1103	68-69	0.36685954482756955
1103	70-71	0.28025129506637114
1103	72-73	0.3419065345325336
1103	74-75	0.3176215966446989
1103	76-77	0.3171096563325335
1103	78-79	0.2960817031721774
1103	80-81	0.3272558328677704
1103	82-83	0.2701507606427853
1103	84-85	0.3090610387107162
1103	86-87	0.36637375891885426
1103	88-89	0.3528500369772658
1103	90-91	0.26530944244745314
1103	92-93	0.23132178049954888
1103	94-95	0.29353669273031713
1103	96-97	0.305032800721154
1103	98-99	0.2989407939507167
1103	100-101	0.2653065358790876
2204	1	0.1296288972301447
2204	2	0.13041482125103698
2204	3	0.10546198341378243
2204	4	0.12407492564804556
2204	5	0.13896939433035982
2204	6	0.16316849714538506
2204	7	0.1775683119237499
2204	8	0.13382008371298326
2204	9	0.08192174848829836
2204	10-11	0.15191694314727044
2204	12-13	0.1307065474793916
2204	14-15	0.1194483061498346
2204	16-17	0.16191265844371117
2204	18-19	0.1297801001440888
2204	20-21	0.08799468174535718
2204	22-23	0.08439664232416533
2204	24-25	-0.09740338010870886
2204	26-27	-0.16734160254756603
2204	28-29	-0.008385054698742067
2204	30-31	0.046605843640513456
2204	32-33	0.0037950527680266077
2204	34-35	-0.24172207145858948
2204	36-37	-0.3782647620205708
2204	38-39	-0.2617887566717272
2204	40-41	-0.07416507588302323
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1113	10-11	-0.22143091299241746
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1113	22-23	-0.3778590511445046
1113	24-25	-0.41770118765438013
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1113	30-31	-0.09340396847819221
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1112	10-11	-0.23869674037430144
1112	12-13	-0.2694135848248038
1112	14-15	-0.44047802908873734
1112	16-17	-0.47336092330677104
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1112	22-23	-0.4249610502468144
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1112	30-31	-0.16148431039837874
1112	32-33	-0.23407377827889775
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1112	86-87	-0.610056738006783
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1112	90-91	-0.6504948775333936
1112	92-93	-0.6932204686028847
1112	94-95	-0.712052214797847
1112	96-97	-0.635799670776958
1112	98-99	-0.6792541146667581
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1115	1	-0.20010597629245908
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1115	10-11	-0.34610353887087797
1115	12-13	-0.2641885382244027
1115	14-15	-0.5603305409699288
1115	16-17	-0.5927101618565942
1115	18-19	-0.6866799968600361
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1115	22-23	-0.639514765170837
1115	24-25	-0.5344178475614498
1115	26-27	-0.517035218196213
1115	28-29	-0.3479954914116945
1115	30-31	-0.24799592471737242
1115	32-33	-0.4310839380181051
1115	34-35	-0.5440350688921995
1115	36-37	-0.5850332005529069
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1115	40-41	-0.6386188174861473
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1213	80-81	-1.556241262713506
1213	82-83	-1.3633807090757344
1213	84-85	-1.3269872076100313
1213	86-87	-1.3611412404369716
1213	88-89	-1.5966038037961
1213	90-91	-1.6763218982164325
1213	92-93	-1.822891636109503
1213	94-95	-1.7971388636025978
1213	96-97	-1.7527976850693214
1213	98-99	-1.9414378369901506
1213	100-101	-1.8917512808539172
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1976.0
3	9170.0
4	6435.0
5	1382.0
6	1201.0
7	3047.0
8	12844.0
9	58908.0
10	294977.0
11	527166.0
12	229567.0
13	84002.0
14	58984.0
15	55158.0
16	57226.0
17	62528.0
18	69612.0
19	79149.0
20	90702.0
21	103092.0
22	116500.0
23	130825.0
24	148363.0
25	168742.0
26	194910.0
27	228862.0
28	271300.0
29	325680.0
30	394510.0
31	495354.0
32	645470.0
33	866553.0
34	1167258.0
35	1624412.0
36	2805816.0
37	7235324.0
38	3895049.0
39	2938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.60166192617329	12.181141205721412	15.259761747260834	34.957435120844465
2	23.116377186109215	23.698239348502035	34.7220554379069	18.463328027481847
3	23.91431267215832	25.94526006117059	25.44486993854425	24.695557328126842
4	25.26168668530524	34.54597770343329	18.78237955964043	21.409956051621037
5	26.364143781449766	33.70767935500926	21.209349637271757	18.718827226269212
6	21.284328462599113	34.56825726002882	25.918002029410914	18.22941224796115
7	18.676405076901972	16.82636737500229	41.83585224834465	22.661375299751086
8	18.901903169805898	23.590490928748324	29.052522625830157	28.45508327561562
9	25.37234487013057	23.113014875670483	28.642421210396414	22.872219043802534
10-11	24.417587175877266	33.743376527779304	20.753018327131638	21.086017969211785
12-13	24.80324009912622	23.343309130203757	28.968787594625	22.884663176045024
14-15	23.650322496286545	26.0468345950473	28.249870699706918	22.05297220895923
16-17	25.676967884461305	25.86320934543079	26.98833074733787	21.471492022770036
18-19	25.099289944377357	26.109945836109063	26.97647161161345	21.814292607900125
20-21	24.465639247678123	27.256414179058012	26.605127963383634	21.672818609880228
22-23	26.69192200512498	26.362372554103104	25.551028144245937	21.394677296525984
24-25	23.81033157962683	29.04101303485744	25.92542224227597	21.22323314323976
26-27	23.110235160540217	29.34261803361013	26.447164805951555	21.099981999898095
28-29	23.931902729911684	28.333721119000316	25.566618513583634	22.16775763750437
30-31	25.929986675347465	26.64848131043212	25.851932072391232	21.569599941829185
32-33	23.175015996292657	27.845786498472158	26.882087935178262	22.09710957005693
34-35	22.64221044238247	29.488046258789996	26.412081965012803	21.45766133381473
36-37	21.69968362510994	28.63601422157146	27.36614427073279	22.298157882585805
38-39	21.670739487551774	26.40216788813476	28.755928458677154	23.17116416563631
40-41	25.509952746336335	26.65795316204741	26.56134459448054	21.270749497135714
42-43	25.819883428737267	26.202396712063624	26.812157541370198	21.16556231782891
44-45	25.914389617286655	26.82520047658674	26.847507827192242	20.412902078934362
46-47	25.318916644512846	25.78085352854393	26.4958296836679	22.40440014327533
48-49	22.730834647483928	26.084249259955357	26.062679304147533	25.122236788413183
50-51	24.34806179030425	26.317816112520433	26.386951122499262	22.947170974676055
52-53	22.89283901825193	27.728370970263168	27.87576346992194	21.50302654156296
54-55	22.45543310768517	27.274701406683082	26.764842268343557	23.505023217288194
56-57	21.43515471816326	26.211119557098016	30.251462740530243	22.102262984208473
58-59	22.129328526713167	28.854840131667714	28.103028809429425	20.912802532189698
60-61	21.494614056257102	31.223248292646588	26.420263977736536	20.861873673359778
62-63	20.655663202434777	31.195165557542065	26.84519850348738	21.303972736535776
64-65	21.129650003042308	31.47029203088247	25.9710706527877	21.428987313287525
66-67	22.205004851802144	31.303279434247827	25.365905170990416	21.125810542959616
68-69	21.307906831339274	30.50981129233558	26.86676175208758	21.315520124237565
70-71	21.790001388175735	29.551705901760133	26.72891154511781	21.92938116494632
72-73	22.15412833876734	29.73255464998912	26.210883722819432	21.90243328842411
74-75	22.468298715506968	29.199233730095603	27.210775770513628	21.121691783883804
76-77	22.699102506355302	27.882415682151024	27.31588289992517	22.102598911568506
78-79	23.153467106257054	28.230269530521024	26.23313323676563	22.38313012645629
80-81	23.689085588999717	27.991765988142514	26.48890020776969	21.83024821508808
82-83	23.073555319076952	28.02103373593664	26.771971004493533	22.133439940492874
84-85	22.888647315349527	28.738715098513072	25.972579097833464	22.40005848830394
86-87	22.363829918450886	29.0261719974663	26.602743369469167	22.00725471461365
88-89	22.47211928659696	28.4027665653753	27.40983730541401	21.71527684261373
90-91	23.427903075601932	27.664083771103957	27.15231964659099	21.75569350670312
92-93	22.666800382329612	27.199748023158882	28.15272544957429	21.980726144937215
94-95	22.924883880953036	27.805221891287797	26.22226967283273	23.047624554926436
96-97	22.230436763598824	29.359698232591608	26.137872964214342	22.271992039595226
98-99	23.13042034339646	28.874575855993157	26.31939198236259	21.6756118182478
100-101	22.901227123228196	27.745136475039423	26.743778933078506	22.609857468653875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1356.0
1	1681.0
2	2814.5
3	4209.5
4	4536.0
5	3724.0
6	2562.5
7	1628.5
8	1266.5
9	1265.5
10	1348.5
11	1439.5
12	1608.5
13	1813.5
14	2150.0
15	2784.0
16	4238.0
17	6219.5
18	7612.0
19	8101.0
20	9085.0
21	11628.0
22	15441.5
23	20081.5
24	28148.5
25	45596.0
26	73996.5
27	101631.5
28	114153.5
29	118419.5
30	128209.0
31	147001.0
32	174885.5
33	204498.0
34	230689.0
35	277033.5
36	396017.0
37	514898.0
38	577264.0
39	608511.5
40	671099.5
41	784317.5
42	891268.5
43	921606.0
44	975986.0
45	1157683.0
46	1388211.5
47	1514626.0
48	1630238.5
49	1745872.0
50	1554350.5
51	1272528.0
52	1064455.0
53	891516.0
54	705432.5
55	443773.5
56	263512.5
57	165870.0
58	98626.0
59	70868.5
60	57788.0
61	62156.5
62	90625.0
63	93458.0
64	62529.5
65	33187.5
66	17252.5
67	11838.0
68	5202.0
69	3096.0
70	2101.5
71	1476.5
72	1219.0
73	1251.0
74	1172.0
75	927.0
76	841.5
77	883.0
78	835.0
79	703.5
80	755.0
81	654.0
82	558.0
83	587.0
84	536.5
85	519.5
86	489.0
87	371.5
88	244.0
89	162.0
90	119.5
91	96.0
92	72.5
93	62.5
94	55.5
95	53.0
96	45.5
97	30.5
98	32.5
99	43.0
100	137.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11666596818325173
2	0.07050390961293126
3	0.05131189391765378
4	0.0350410779280188
5	0.028159832420806184
6	0.027267490261483777
7	0.033558280509045246
8	0.04545173645344691
9	0.06128748014649683
10-11	0.0761820470346893
12-13	0.09482578284600501
14-15	0.11117872983040349
16-17	0.13080581782226602
18-19	0.14722535750512142
20-21	0.17257275829443136
22-23	0.1932986258108327
24-25	0.22123204305688543
26-27	0.23483693135162936
28-29	0.2656293951180982
30-31	0.2974274086312661
32-33	0.30635970925095113
34-35	0.29049288896528797
36-37	0.24685691342309912
38-39	0.21556278466158835
40-41	0.1910389135765287
42-43	0.16897009330791327
44-45	0.15381803465235416
46-47	0.1448768550062082
48-49	0.13647507621756316
50-51	0.1326393367864459
52-53	0.12845953507996807
54-55	0.12079693524419453
56-57	0.11751391521026956
58-59	0.11983800038641522
60-61	0.11732745565459024
62-63	0.1155028157168713
64-65	0.11376252653053107
66-67	0.111453979650692
68-69	0.11042179282461011
70-71	0.10760714143649862
72-73	0.10016873701886332
74-75	0.0939933741152938
76-77	0.08773809997357603
78-79	0.0852031379189835
80-81	0.07928526678278065
82-83	0.07740513292968096
84-85	0.07959159319568238
86-87	0.08346284873264329
88-89	0.09221090955326422
90-91	0.09917872556847078
92-93	0.10881912854841413
94-95	0.11873478134864598
96-97	0.13164710557943815
98-99	0.14159605472889847
100-101	0.15083024224825475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.2524992E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.070488821820142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84146261318669	17.03369134330741
2	11.007869779071642	4.638823942639616
3	3.086112264759615	1.9507768193249855
4	1.5815977852861096	1.3330015382194587
5	0.7548043254244521	0.7952048050758704
6	0.5211383327314977	0.6588383648664605
7	0.30514174124912136	0.45006399536422426
8	0.2479968670751708	0.4180332172443041
9	0.190820063272459	0.36186048091452233
>10	1.0852731700008897	4.16551347873839
>50	0.1167060004220987	1.7522717429914298
>100	0.14773984624741437	7.125027370466376
>500	0.04512292832347441	6.805889553489646
>1k	0.0555822028308345	25.52972055626903
>5k	0.008524158065973866	12.430196082439751
>10k+	0.00410792205267191	14.551086708648416
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	63100	0.28013328484201017	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	50229	0.22299231005276274	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	45003	0.19979141391038008	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	42373	0.18811549411427095	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	40000	0.17758052921838996	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	39181	0.17394456788264342	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	38122	0.16924312337158656	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	37097	0.1646926223103653	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	36931	0.16395566311410897	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	36079	0.16017319784175726	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	33529	0.1488524391040849	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	33140	0.14712546845743607	No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT	31777	0.14107441192431944	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	28610	0.12701447352345344	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	28286	0.12557607123678444	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	28216	0.12526530531065228	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	27938	0.12403112063258447	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	27112	0.12036408270422472	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	26765	0.11882357161325519	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	26327	0.11687906481831381	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	26296	0.11674143990816956	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	25950	0.11520536833043048	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	25836	0.11469926382215807	No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	24958	0.11080137120581442	No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA	24264	0.10772034902387535	No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC	23654	0.10501224595329489	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	23100	0.1025527556236202	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	23061	0.10237961460763227	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	22708	0.10081246643727998	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	22583	0.1002575272834725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.012057717933928678	0.0	0.0
2	0.012284133108682125	0.0	0.0
3	0.013314100178148786	0.0	0.0
4	0.013718095882120624	0.0	0.0
5	0.015263046486320616	0.0	0.0
6	0.01574695342844073	0.0	0.0
7	0.01664817461422406	0.0	0.0
8	0.017243069387105663	0.0	4.439513230459749E-6
9	0.017913435884905085	0.0	8.879026460919497E-6
10-11	0.02030189400289243	2.2197566152298743E-6	1.553829630660912E-5
12-13	0.02265705577165133	4.439513230459749E-6	1.7758052921838995E-5
14-15	0.02458602427028609	4.439513230459749E-6	1.9977809537068872E-5
16-17	0.025953394345267694	4.439513230459749E-6	2.6637079382758494E-5
18-19	0.026756946239980907	4.439513230459749E-6	2.6637079382758494E-5
20-21	0.027502784462698146	8.879026460919497E-6	2.6637079382758494E-5
22-23	0.02811987680173205	1.3318539691379247E-5	3.551610584367799E-5
24-25	0.028688134495230895	1.3318539691379247E-5	3.995561907413774E-5
26-27	0.029413994908411067	1.3318539691379247E-5	3.995561907413774E-5
28-29	0.031129866771983757	2.441732276752862E-5	4.217537568936762E-5
30-31	0.035354063610766207	3.107659261321824E-5	4.439513230459749E-5
32-33	0.037591578278917924	3.3296349228448115E-5	4.883464553505724E-5
34-35	0.03973586316922999	3.551610584367799E-5	4.883464553505724E-5
36-37	0.04182021463093083	3.551610584367799E-5	5.327415876551699E-5
38-39	0.044574932590431104	3.773586245890786E-5	5.327415876551699E-5
40-41	0.0481332024446446	3.995561907413774E-5	5.7713671995976735E-5
42-43	0.05289014087108222	3.995561907413774E-5	5.7713671995976735E-5
44-45	0.0576737163769026	4.439513230459749E-5	5.7713671995976735E-5
46-47	0.06328526110020372	4.439513230459749E-5	5.7713671995976735E-5
48-49	0.06998670632158271	4.883464553505724E-5	5.7713671995976735E-5
50-51	0.07820868482439416	4.883464553505724E-5	7.991123814827547E-5
52-53	0.08823310569877227	4.883464553505724E-5	7.991123814827547E-5
54-55	0.10098782720988314	4.883464553505724E-5	7.991123814827547E-5
56-57	0.11351835330285578	4.883464553505724E-5	7.991123814827547E-5
58-59	0.12798006765107842	4.883464553505724E-5	8.65705079939651E-5
60-61	0.14578695521845247	5.1054402150287114E-5	8.879026460919498E-5
62-63	0.16924978264143226	5.7713671995976735E-5	8.879026460919498E-5
64-65	0.20018209107466053	6.215318522643648E-5	9.101002122442486E-5
66-67	0.23412660923475578	6.215318522643648E-5	9.322977783965473E-5
68-69	0.2704928818620668	6.215318522643648E-5	1.0210880430057423E-4
70-71	0.31749844794617466	6.437294184166636E-5	1.0210880430057423E-4
72-73	0.3815295472690956	6.88124550721261E-5	1.0210880430057423E-4
74-75	0.453280960099786	7.103221168735598E-5	1.0210880430057423E-4
76-77	0.5237382548237974	7.103221168735598E-5	1.0210880430057423E-4
78-79	0.6076184178001041	7.103221168735598E-5	1.0654831753103397E-4
80-81	0.7114275556679444	7.103221168735598E-5	1.0654831753103397E-4
82-83	0.8331789862566876	7.103221168735598E-5	1.0654831753103397E-4
84-85	0.9698405220299302	7.103221168735598E-5	1.0654831753103397E-4
86-87	1.1210059475270846	7.103221168735598E-5	1.0654831753103397E-4
88-89	1.290297905544206	7.103221168735598E-5	1.0654831753103397E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGC	7445	0.0	77.930176	9
GGGAGAG	32150	0.0	74.8932	5
AAGGGGC	13290	0.0	74.73703	9
GAGGGGC	23230	0.0	70.16553	9
GGAGAGC	10325	0.0	69.330536	6
ACGGGAG	6890	0.0	68.49024	3
GAGAGCG	10020	0.0	67.65499	7
AGAGCGG	10070	0.0	66.98922	8
AACGGGA	11445	0.0	65.459694	2
GATTGGG	6555	0.0	63.537815	1
CGGGAAA	21845	0.0	56.9582	4
CGGGAGA	36505	0.0	56.855053	4
AAGCGGC	5645	0.0	56.688522	9
GGAGAGG	36035	0.0	54.523552	6
TTGGGAG	5575	0.0	53.47814	3
TCCAATC	7770	0.0	49.55444	2
GTCCAAT	7830	0.0	49.49186	1
TAGGGAG	4095	0.0	48.923786	3
TCGGGAG	37705	0.0	48.425247	3
ATTGGGA	8645	0.0	47.33965	2
>>END_MODULE