FastQCFastQC Report
Thu 14 Aug 2014
brugia_1_ATCACG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebrugia_1_ATCACG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22524992
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA631000.28013328484201017No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC502290.22299231005276274No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT450030.19979141391038008No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT423730.18811549411427095No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG400000.17758052921838996No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG391810.17394456788264342No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC381220.16924312337158656No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA370970.1646926223103653No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG369310.16395566311410897No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC360790.16017319784175726No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC335290.1488524391040849No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT331400.14712546845743607No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT317770.14107441192431944No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG286100.12701447352345344No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG282860.12557607123678444No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA282160.12526530531065228No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG279380.12403112063258447No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG271120.12036408270422472No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG267650.11882357161325519No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC263270.11687906481831381No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC262960.11674143990816956No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC259500.11520536833043048No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG258360.11469926382215807No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC249580.11080137120581442No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA242640.10772034902387535No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC236540.10501224595329489No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT231000.1025527556236202No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG230610.10237961460763227No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC227080.10081246643727998No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA225830.1002575272834725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC74450.077.9301769
GGGAGAG321500.074.89325
AAGGGGC132900.074.737039
GAGGGGC232300.070.165539
GGAGAGC103250.069.3305366
ACGGGAG68900.068.490243
GAGAGCG100200.067.654997
AGAGCGG100700.066.989228
AACGGGA114450.065.4596942
GATTGGG65550.063.5378151
CGGGAAA218450.056.95824
CGGGAGA365050.056.8550534
AAGCGGC56450.056.6885229
GGAGAGG360350.054.5235526
TTGGGAG55750.053.478143
TCCAATC77700.049.554442
GTCCAAT78300.049.491861
TAGGGAG40950.048.9237863
TCGGGAG377050.048.4252473
ATTGGGA86450.047.339652
Produced by FastQC (version 0.11.2)