##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_1_ATCACG_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22524992
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.695296317974275	33.0	33.0	33.0	33.0	33.0
2	32.66017208796345	33.0	33.0	33.0	33.0	33.0
3	32.64995152939455	33.0	33.0	33.0	33.0	33.0
4	36.52779375015982	37.0	37.0	37.0	37.0	37.0
5	36.42167175908431	37.0	37.0	37.0	37.0	37.0
6	36.37514423978486	37.0	37.0	37.0	37.0	37.0
7	36.36777327157319	37.0	37.0	37.0	37.0	37.0
8	36.35545877219401	37.0	37.0	37.0	37.0	37.0
9	36.35970228091535	37.0	37.0	37.0	37.0	37.0
10-11	36.34280988867832	37.0	37.0	37.0	37.0	37.0
12-13	36.33188522331107	37.0	37.0	37.0	37.0	37.0
14-15	38.289771334879944	40.0	37.0	40.0	37.0	40.0
16-17	38.3092820188349	40.0	37.0	40.0	37.0	40.0
18-19	38.290010247284435	40.0	37.0	40.0	37.0	40.0
20-21	38.31650246535048	40.0	37.0	40.0	37.0	40.0
22-23	38.25109944545152	40.0	37.0	40.0	37.0	40.0
24-25	38.21833179785369	40.0	37.0	40.0	37.0	40.0
26-27	38.15822782534174	40.0	37.0	40.0	37.0	40.0
28-29	38.09508558316025	40.0	37.0	40.0	33.0	40.0
30-31	37.992367056112606	40.0	37.0	40.0	33.0	40.0
32-33	37.87837323094277	40.0	37.0	40.0	33.0	40.0
34-35	37.83749821087616	40.0	37.0	40.0	33.0	40.0
36-37	37.802681217378456	40.0	37.0	40.0	33.0	40.0
38-39	37.74100612333181	40.0	37.0	40.0	33.0	40.0
40-41	37.52574598028714	40.0	37.0	40.0	33.0	40.0
42-43	37.23027650797834	40.0	37.0	40.0	33.0	40.0
44-45	37.41445970324873	40.0	37.0	40.0	33.0	40.0
46-47	37.30831573658273	40.0	37.0	40.0	33.0	40.0
48-49	37.2180020530085	40.0	37.0	40.0	33.0	40.0
50-51	36.98601744231474	38.5	37.0	40.0	33.0	40.0
52-53	36.74986150938477	37.0	37.0	40.0	33.0	40.0
54-55	36.5992709120607	37.0	37.0	40.0	33.0	40.0
56-57	36.376661243653274	37.0	37.0	40.0	33.0	40.0
58-59	36.12187154161919	37.0	37.0	40.0	33.0	40.0
60-61	35.914440946305334	37.0	37.0	38.5	33.0	40.0
62-63	35.82142446487883	37.0	37.0	37.0	33.0	40.0
64-65	35.472759790547315	37.0	33.0	37.0	33.0	40.0
66-67	35.34600076661514	37.0	33.0	37.0	33.0	40.0
68-69	35.06497644927022	37.0	33.0	37.0	33.0	38.5
70-71	34.73404028289999	37.0	33.0	37.0	27.0	37.0
72-73	34.3455841626936	37.0	33.0	37.0	27.0	37.0
74-75	33.6403704161138	37.0	33.0	37.0	27.0	37.0
76-77	33.39031099322921	37.0	33.0	37.0	27.0	37.0
78-79	33.32565984929096	37.0	33.0	37.0	27.0	37.0
80-81	33.31870643949618	37.0	33.0	37.0	27.0	37.0
82-83	33.40435446103599	37.0	33.0	37.0	27.0	37.0
84-85	33.34449710348399	37.0	33.0	37.0	27.0	37.0
86-87	33.30149093948624	37.0	33.0	37.0	27.0	37.0
88-89	33.27967159766361	37.0	33.0	37.0	27.0	37.0
90-91	33.238563059201084	37.0	33.0	37.0	27.0	37.0
92-93	33.21249508101934	37.0	33.0	37.0	27.0	37.0
94-95	33.17635069526329	37.0	33.0	37.0	27.0	37.0
96-97	33.1041088716036	37.0	33.0	37.0	27.0	37.0
98-99	33.048724811977735	37.0	33.0	37.0	27.0	37.0
100-101	32.66382809370143	35.0	33.0	37.0	24.5	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.07752098217873993
2201	2	0.10260749008830317
2201	3	0.12041713527117537
2201	4	0.12318185695303896
2201	5	0.13582543258382174
2201	6	0.19863998290003337
2201	7	0.16256073528737858
2201	8	0.13192995524042317
2201	9	0.1864127901040149
2201	10-11	0.1928099890614945
2201	12-13	0.2020171612302022
2201	14-15	0.3792301843820667
2201	16-17	0.4393623370325983
2201	18-19	0.4300559768749963
2201	20-21	0.4157339639704105
2201	22-23	0.3038261123495545
2201	24-25	0.07521102044799477
2201	26-27	0.11580116648003269
2201	28-29	0.1912847469234933
2201	30-31	0.12707251113506146
2201	32-33	0.2703301322434868
2201	34-35	0.1334058638051019
2201	36-37	0.33126350689712325
2201	38-39	0.45690785536103107
2201	40-41	0.465158369717642
2201	42-43	0.42234171364464856
2201	44-45	0.4731237537343773
2201	46-47	0.47203107301028524
2201	48-49	0.4434967777551222
2201	50-51	0.42451773903088963
2201	52-53	0.5066957893063844
2201	54-55	0.4485114451249004
2201	56-57	0.42176912123171917
2201	58-59	0.4838338822837329
2201	60-61	0.48603400995665424
2201	62-63	0.4511284033944776
2201	64-65	0.44834735271046355
2201	66-67	0.4198941819588953
2201	68-69	0.3263191833305328
2201	70-71	0.4056574727004474
2201	72-73	0.4654225834427592
2201	74-75	0.4960857374854939
2201	76-77	0.37758848244848053
2201	78-79	0.38501524552259525
2201	80-81	0.22394625966784787
2201	82-83	0.2096246962919821
2201	84-85	0.344026341434585
2201	86-87	0.41693515665350134
2201	88-89	0.3937303353112611
2201	90-91	0.41927413055200446
2201	92-93	0.4341796242005813
2201	94-95	0.45918466842423555
2201	96-97	0.5056217981412416
2201	98-99	0.5100195296716734
2201	100-101	0.53281252158407
1101	1	0.08590999451863723
1101	2	0.06939667923224135
1101	3	0.10153934569869705
1101	4	0.11323919052696851
1101	5	0.153502644009194
1101	6	0.139781099391449
1101	7	0.16120424428094537
1101	8	0.17595900699966904
1101	9	0.17843196884685852
1101	10-11	0.1853839082973181
1101	12-13	0.08003479244428036
1101	14-15	0.08299589626560788
1101	16-17	0.051800429958781535
1101	18-19	0.22048251363968063
1101	20-21	0.1108310435938975
1101	22-23	0.2096528226386738
1101	24-25	0.3143986709142652
1101	26-27	0.3234394541591641
1101	28-29	0.3480029139284895
1101	30-31	0.38797276968451655
1101	32-33	0.3514345630709954
1101	34-35	0.34021607271719034
1101	36-37	0.3937783286362162
1101	38-39	0.4165874780962824
1101	40-41	0.37001123769988453
1101	42-43	0.3792125058270699
1101	44-45	0.2918033501945416
1101	46-47	0.3944618913500477
1101	48-49	0.4071267710751627
1101	50-51	0.3787485319956403
1101	52-53	0.2707531976517856
1101	54-55	0.3414737834120771
1101	56-57	0.36887283000277193
1101	58-59	0.29573258800856195
1101	60-61	0.39453090581960737
1101	62-63	0.33746778856512094
1101	64-65	0.3961374251642269
1101	66-67	0.39771003887370426
1101	68-69	0.30835916915670225
1101	70-71	0.36724897456037553
1101	72-73	0.27276461052891676
1101	74-75	0.30941270577192626
1101	76-77	0.34938243673195757
1101	78-79	0.31627745743287505
1101	80-81	0.3535466519488324
1101	82-83	0.34706847183747414
1101	84-85	0.3619067678861967
1101	86-87	0.3282380777565379
1101	88-89	0.28427829043543085
1101	90-91	0.26087196663372225
1101	92-93	0.29710849053397226
1101	94-95	0.3261690280038536
1101	96-97	0.21703214464424292
1101	98-99	0.3138627256119051
1101	100-101	0.3358189314408193
2202	1	0.061994448222385756
2202	2	0.052137262259478234
2202	3	0.07322349441265175
2202	4	0.07865220377951232
2202	5	0.12211701157570332
2202	6	0.15215769222586317
2202	7	0.12647830152024397
2202	8	0.09505733870732769
2202	9	0.10353384041764713
2202	10-11	0.13133854588688365
2202	12-13	0.13829089140193673
2202	14-15	0.20210379328237593
2202	16-17	0.19555424644133979
2202	18-19	0.16840976373629957
2202	20-21	0.23906780002282346
2202	22-23	0.21081290845294376
2202	24-25	0.22777441226443074
2202	26-27	0.26573168639843203
2202	28-29	0.2444584479247922
2202	30-31	0.22569818379345463
2202	32-33	0.12216368576068248
2202	34-35	0.19946739265466817
2202	36-37	0.31039121195717456
2202	38-39	0.3227122423061033
2202	40-41	0.22557246151885835
2202	42-43	0.21836554736250946
2202	44-45	0.3122949852030743
2202	46-47	0.23645954026376614
2202	48-49	0.26426942347847415
2202	50-51	0.20318678078390917
2202	52-53	0.2994566636697513
2202	54-55	0.24360688034600742
2202	56-57	0.31256802211900947
2202	58-59	0.3724579251639568
2202	60-61	0.32168236279731843
2202	62-63	0.3224675501912557
2202	64-65	0.3005632158468359
2202	66-67	0.3403385104806489
2202	68-69	0.21069403232426254
2202	70-71	0.2786109853883616
2202	72-73	0.27172514991806906
2202	74-75	0.3062143818068819
2202	76-77	0.29025385906585655
2202	78-79	0.2491722669872729
2202	80-81	0.2551301444299767
2202	82-83	0.2822013667250616
2202	84-85	0.23688910884615666
2202	86-87	0.226028389288345
2202	88-89	0.22788775881900136
2202	90-91	0.23382912673258005
2202	92-93	0.24060058000724638
2202	94-95	0.2688091921739115
2202	96-97	0.3416434598681164
2202	98-99	0.3118465382152422
2202	100-101	0.3190538423006686
1102	1	0.058200689840717246
1102	2	0.05039791295507712
1102	3	0.0768364629229552
1102	4	0.09859606923669162
1102	5	0.1000869836173095
1102	6	0.1273203887314338
1102	7	0.10217589073850775
1102	8	0.11414733265905852
1102	9	0.07976853599293321
1102	10-11	0.13517502894185185
1102	12-13	0.14487589478947882
1102	14-15	0.26201426828885843
1102	16-17	0.23696318237647773
1102	18-19	0.2752621619845286
1102	20-21	0.24986867567900362
1102	22-23	0.2706403952038059
1102	24-25	0.21650217749442646
1102	26-27	0.24767286281945644
1102	28-29	0.24895139690580237
1102	30-31	0.25242594289748155
1102	32-33	0.3218287907982358
1102	34-35	0.2526767649813664
1102	36-37	0.3139193263303852
1102	38-39	0.34764621049925637
1102	40-41	0.30295044955584416
1102	42-43	0.28650476033851646
1102	44-45	0.22391464978018405
1102	46-47	0.2734761276558828
1102	48-49	0.3133593307570095
1102	50-51	0.3493096576264634
1102	52-53	0.36926308187671
1102	54-55	0.3110583053317342
1102	56-57	0.2597861640351766
1102	58-59	0.349696220873966
1102	60-61	0.30304600208532406
1102	62-63	0.282355234692659
1102	64-65	0.28436306770254305
1102	66-67	0.29558165817803683
1102	68-69	0.22981030912268352
1102	70-71	0.2965333321092416
1102	72-73	0.2710164526860126
1102	74-75	0.2122370372729705
1102	76-77	0.24637531864101447
1102	78-79	0.25610801931088645
1102	80-81	0.28856567389779286
1102	82-83	0.26201223148140684
1102	84-85	0.3193845410144718
1102	86-87	0.32651429291488654
1102	88-89	0.30872944150382864
1102	90-91	0.25698955785740196
1102	92-93	0.2255091433211689
1102	94-95	0.2645048242898227
1102	96-97	0.3090183549557537
1102	98-99	0.21972238850712955
1102	100-101	0.2936452480291578
2203	1	0.030943582505983613
2203	2	0.05305765715030475
2203	3	0.03994812022035177
2203	4	0.04873266238593743
2203	5	0.07540905136720255
2203	6	0.09623552212452324
2203	7	0.07474758562263872
2203	8	0.04665537571177936
2203	9	0.09587047226632706
2203	10-11	0.11317082864284345
2203	12-13	0.103562567630199
2203	14-15	0.18764299587027722
2203	16-17	0.1627561349755382
2203	18-19	0.19885706032570738
2203	20-21	0.19488175886457526
2203	22-23	0.23190447405605852
2203	24-25	0.2056166229818075
2203	26-27	0.19133776472732933
2203	28-29	0.16962551559735317
2203	30-31	0.20072126423820436
2203	32-33	0.1937654808883238
2203	34-35	0.22459123649409918
2203	36-37	0.2162273680768152
2203	38-39	0.17685065647275167
2203	40-41	0.1750343650334969
2203	42-43	0.11146246550589467
2203	44-45	0.16774319730701137
2203	46-47	0.18635809324227637
2203	48-49	0.17995012350089468
2203	50-51	0.2243057262519983
2203	52-53	0.23326026905774455
2203	54-55	0.18336436934888667
2203	56-57	0.10424004966111511
2203	58-59	0.20615545386300482
2203	60-61	0.14216724524517588
2203	62-63	0.09834045718549334
2203	64-65	0.15153682906311872
2203	66-67	0.13390943890431117
2203	68-69	0.08352816718040401
2203	70-71	0.1310761632252948
2203	72-73	0.11799866707528395
2203	74-75	0.07167067244262171
2203	76-77	0.08696648900027526
2203	78-79	0.07483140416004375
2203	80-81	0.08119954420015318
2203	82-83	0.06901251331981939
2203	84-85	0.09084763606809076
2203	86-87	0.021537507858191418
2203	88-89	0.011154243982097967
2203	90-91	0.010779855553607831
2203	92-93	-0.009414988253375611
2203	94-95	0.06500520053207737
2203	96-97	0.07389014835727892
2203	98-99	0.047876109041141035
2203	100-101	0.10892231092259408
1103	1	0.04554863835719658
1103	2	0.03794943596612654
1103	3	0.04636024300705799
1103	4	0.054107721780894735
1103	5	0.07409550929958897
1103	6	0.10737716609903458
1103	7	0.021154633924169275
1103	8	0.07802992443880186
1103	9	0.07685266304535787
1103	10-11	0.06203632956410132
1103	12-13	0.07863338508148843
1103	14-15	0.15981401409548823
1103	16-17	0.1522986353573188
1103	18-19	0.060543089226960944
1103	20-21	0.13354394434200145
1103	22-23	0.11845253649685361
1103	24-25	0.0693128762760793
1103	26-27	0.1218311464771702
1103	28-29	0.09376511978252466
1103	30-31	0.03251870878927576
1103	32-33	0.12922626600740017
1103	34-35	0.10273709062121128
1103	36-37	0.10131597975442475
1103	38-39	0.14549324662727514
1103	40-41	0.1171289005236531
1103	42-43	0.11629988871041519
1103	44-45	0.09766643542771192
1103	46-47	0.1467936162625847
1103	48-49	0.17342458936375493
1103	50-51	0.23371222940245673
1103	52-53	0.17838915544812295
1103	54-55	0.1426212780709193
1103	56-57	0.012140407790099061
1103	58-59	0.1492980915994906
1103	60-61	0.1585725779207081
1103	62-63	0.09150949074805226
1103	64-65	0.13781439620847635
1103	66-67	0.04911124933543931
1103	68-69	-0.02651037301822612
1103	70-71	0.1100244794995362
1103	72-73	0.08648361190677889
1103	74-75	0.1475749555378627
1103	76-77	0.16942992308634786
1103	78-79	0.1510354096670099
1103	80-81	0.12139308229253487
1103	82-83	0.1286338785542327
1103	84-85	0.11533605217127985
1103	86-87	0.14395856171282162
1103	88-89	0.17030967081219472
1103	90-91	0.09846021132094762
1103	92-93	0.006157676696851411
1103	94-95	0.16106809487863671
1103	96-97	0.22704119663541888
1103	98-99	0.21956247196056466
1103	100-101	0.23712764689548038
2204	1	0.021290635817521775
2204	2	0.03810907162749544
2204	3	0.04954350535456342
2204	4	0.03217432900302697
2204	5	0.06425885721842661
2204	6	0.04376041463246594
2204	7	0.042362240474105306
2204	8	0.061526558543882004
2204	9	0.05750682136360297
2204	10-11	0.04501315765538294
2204	12-13	0.05496311357738648
2204	14-15	0.03965151778936615
2204	16-17	0.08632530709558495
2204	18-19	0.07450991895326808
2204	20-21	0.10228108201523156
2204	22-23	0.10747589067641883
2204	24-25	0.13660067315825586
2204	26-27	0.11345800244219362
2204	28-29	0.16562208793103395
2204	30-31	0.12385174777859476
2204	32-33	0.14372402449100719
2204	34-35	0.19785564071744943
2204	36-37	0.12857604969015313
2204	38-39	0.13129589201663094
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1213	46-47	-0.6339239248329207
1213	48-49	-0.6329080243587342
1213	50-51	-0.6640832183180407
1213	52-53	-0.5977694053174574
1213	54-55	-0.579938922684569
1213	56-57	-0.5988013472529445
1213	58-59	-0.5943515587429289
1213	60-61	-0.563648666758013
1213	62-63	-0.7705686832839405
1213	64-65	-0.7658564179032226
1213	66-67	-0.730841608756343
1213	68-69	-0.7181620044089101
1213	70-71	-0.8446399502868402
1213	72-73	-0.897700033812157
1213	74-75	-0.5592836440990254
1213	76-77	-0.526964667557074
1213	78-79	-0.6145119702237523
1213	80-81	-0.48858847996877586
1213	82-83	-0.49900392152066786
1213	84-85	-0.3915220047494188
1213	86-87	-0.52424914805664
1213	88-89	-0.6181750901273588
1213	90-91	-0.5599808935436457
1213	92-93	-0.6422276535683906
1213	94-95	-0.7195345652029985
1213	96-97	-0.7230986403770743
1213	98-99	-0.8045930117086186
1213	100-101	-0.7009133455314434
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	11.0
12	66.0
13	267.0
14	977.0
15	2459.0
16	4840.0
17	8271.0
18	13760.0
19	22343.0
20	33936.0
21	47943.0
22	65779.0
23	87360.0
24	116291.0
25	154490.0
26	202285.0
27	273371.0
28	385598.0
29	621373.0
30	531347.0
31	460122.0
32	602150.0
33	817014.0
34	1117107.0
35	1541751.0
36	2639728.0
37	7820710.0
38	4949668.0
39	3968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.94667247486555	11.483883802274345	15.945761724413396	35.623681998446706
2	22.288269847110268	23.000638579583068	35.434205703602466	19.276885869704195
3	23.49869057565212	22.547414409697048	28.57436707160717	25.379527943043662
4	25.529358678573562	31.390759206484958	19.35112340994394	23.728758704997542
5	27.434417883850447	32.90492069443011	21.375536812635186	18.285124609084253
6	21.26914406895239	34.51239405545627	25.92375615494114	18.2947057206502
7	15.084862183302883	20.602320302710872	41.98360203635145	22.329215477634797
8	18.869098821433543	24.066543508650305	28.744844837236787	28.31951283267936
9	27.021203825510792	21.13448031413285	28.86467173884013	22.979644121516223
10-11	24.310168456441627	33.55654243961552	21.0190241133049	21.114264990637956
12-13	21.111900160981268	25.20322436675973	29.11097239105329	24.573903081205707
14-15	21.602995463882962	27.88563254539669	27.70119518799385	22.810176802726502
16-17	24.39653913275877	25.7017561384045	26.375794173493528	23.5259105553432
18-19	21.5133061978446	26.000268945711504	28.403692662798726	24.082732193645175
20-21	24.204223912709935	25.954905999522666	28.31575478472978	21.52511530303762
22-23	21.946957740212277	30.94085797978183	25.61625684689751	21.495927433108385
24-25	21.450866664014796	26.69014044488895	28.252340777745893	23.606652113350364
26-27	22.128718181120774	25.58889921026387	26.437558779155175	25.844823829460182
28-29	23.996304104497284	28.149309738795637	25.6428377596214	22.211548397085682
30-31	21.715835494186813	26.159175479800485	28.159620987613348	23.965368038399355
32-33	21.032792057046713	28.288966223233825	26.31977216903007	24.358469550689392
34-35	20.48022665337416	29.379275921723842	28.63217164170888	21.508325783193122
36-37	21.258038448258315	29.227792872769182	25.721416220836918	23.792752458135585
38-39	23.704074386953163	26.04712729603232	26.457758226242373	23.791040090772146
40-41	21.659826328651697	28.743908035526278	28.859870510752096	20.73639512506993
42-43	21.61910873636542	26.18201299579112	28.975426620705854	23.223451647137612
44-45	23.757220706596062	26.724597765405044	26.770307024064888	22.74787450393401
46-47	21.964231837382396	27.910149358318787	28.535475331024895	21.590143473273923
48-49	23.801911177509457	25.877558008073603	28.471362339151597	21.849168475265344
50-51	21.1143061981285	25.991264277860793	26.239107416439616	26.655322107571095
52-53	24.359043741965433	26.01033547931794	28.3575745892881	21.27304618942853
54-55	22.21738807990627	25.44914219735431	28.198158616491554	24.135311106247865
56-57	21.278681058865086	25.710010869798776	28.710839378967073	24.30046869236907
58-59	24.207121243144066	25.791217654585775	29.056467261850567	20.94519384041959
60-61	21.39532697829898	26.523055212503145	28.87013087603345	23.211486933164426
62-63	22.854160743727693	26.527365654201716	29.281791468827738	21.33668213324285
64-65	20.981545671923147	31.46099639153195	26.10098062249593	21.456477314048975
66-67	21.981108706021416	31.52744935609445	25.351598133375724	21.139843804508406
68-69	21.062439151664854	31.00867674312174	26.773917858856922	21.15496624635648
70-71	21.269518404458374	30.730203859575433	26.42872368802997	21.571554047936225
72-73	21.378420620351456	31.24303787133841	25.82626509104477	21.552276417265368
74-75	21.800797186659086	30.170695507912708	26.890354936432082	21.13815236899612
76-77	22.25447813458322	28.47753968139456	27.165297245582355	22.10268493843987
78-79	22.781312620751994	28.570136341025865	26.173881362359413	22.474669675862728
80-81	23.265966424460007	28.285206776624563	26.41638151917491	22.03244527974052
82-83	22.70501666711212	28.2333269608708	26.767044198814084	22.294612173203
84-85	22.450357691011618	28.94404454344171	25.965319533307905	22.640278232238764
86-87	21.98050699743118	29.166259693002743	26.494280762103323	22.35895254746275
88-89	22.19757119107198	28.477161344882397	27.38248492871725	21.942782535328377
90-91	22.864254496218408	27.808548120785115	27.242888303051842	22.084309079944635
92-93	22.23535066385311	27.240364666874612	28.207797512868016	22.31648715640426
94-95	22.515666344981273	27.82344422710145	26.361045564104973	23.299843863812303
96-97	21.74335746628794	29.351311472185703	26.167014749653063	22.738316311873298
98-99	22.647345558692745	28.638586824533085	26.541347364641116	22.172720252133054
100-101	22.47418828921991	27.559189932436656	26.915279829101017	23.051341949242417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	796.0
1	983.0
2	967.0
3	743.5
4	632.5
5	493.5
6	424.5
7	457.5
8	491.0
9	514.0
10	557.0
11	614.5
12	699.0
13	843.5
14	1133.5
15	1646.5
16	3148.5
17	5160.5
18	6676.0
19	7093.5
20	7597.5
21	9939.5
22	13403.0
23	18285.5
24	26856.5
25	44161.0
26	72026.5
27	100387.0
28	112232.5
29	114323.0
30	123937.5
31	144346.0
32	172152.5
33	200341.0
34	227268.5
35	275596.5
36	410360.5
37	537954.5
38	602462.5
39	649086.5
40	735132.0
41	875118.0
42	997551.5
43	1020826.0
44	1049494.0
45	1204916.0
46	1395590.0
47	1482556.5
48	1573038.5
49	1669067.0
50	1482660.5
51	1214864.0
52	1010429.5
53	848825.0
54	675338.5
55	416841.5
56	242256.0
57	151590.0
58	88055.5
59	62989.0
60	51211.5
61	57340.5
62	93234.5
63	97509.0
64	60702.0
65	29485.5
66	15128.5
67	9963.5
68	2606.5
69	1775.5
70	1347.5
71	1034.5
72	818.0
73	895.0
74	918.5
75	706.5
76	689.0
77	746.5
78	627.5
79	477.5
80	584.5
81	521.5
82	453.5
83	492.0
84	458.5
85	477.0
86	452.5
87	331.5
88	182.0
89	97.5
90	56.0
91	38.5
92	23.0
93	25.5
94	25.5
95	13.5
96	5.5
97	1.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02265039650180564
2	0.0
3	0.0060111009140425
4	0.0
5	0.009926751583307998
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.883464553505723E-5
14-15	0.0
16-17	8.879026460919499E-6
18-19	0.0
20-21	0.0
22-23	3.995561907413774E-5
24-25	0.0
26-27	0.0
28-29	1.997780953706887E-5
30-31	5.971145294968363E-4
32-33	0.002548280594283896
34-35	1.0654831753103397E-4
36-37	1.9755833875545882E-4
38-39	9.101002122442486E-5
40-41	1.465039366051717E-4
42-43	3.107659261321824E-5
44-45	6.659269845689623E-5
46-47	4.439513230459749E-4
48-49	0.005942288458970374
50-51	0.05847948802823104
52-53	0.0019711438743241286
54-55	1.1764710060718334E-4
56-57	8.701445931701108E-4
58-59	0.04360489894957565
60-61	0.00938291121257668
62-63	0.013731414421812005
64-65	0.02179357044832691
66-67	0.005462821030080721
68-69	0.00105882390546465
70-71	6.6592698456896234E-6
72-73	1.1098783076149371E-5
74-75	1.2874588368333271E-4
76-77	0.001185350032532753
78-79	0.0011564931965347646
80-81	0.004332964912928715
82-83	0.00441287615107699
84-85	0.0060466170198861776
86-87	0.004492787389225266
88-89	0.004572698627373541
90-91	6.503886882623533E-4
92-93	0.01361598707782005
94-95	0.01326082601938327
96-97	8.47947027017812E-4
98-99	0.0073074387773367465
100-101	0.002550500350899126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.2524992E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.06136902896088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.34382445980134	12.261863377310792
2	13.055808065945179	4.1938830263685904
3	4.278744885520074	2.061675017613506
4	1.7672080381711925	1.1353512180805403
5	1.0830306970303438	0.8697477847348537
6	0.6715084206800211	0.6471206730357915
7	0.3710436019751548	0.41716277520104866
8	0.3415620548060918	0.43887633668246406
9	0.2290233978164942	0.33105863777375033
>10	1.3598871815231162	3.9753350681320563
>50	0.158319755411544	1.8288944388008797
>100	0.1925988525080764	6.894264896516589
>500	0.054814166942312295	6.305615189179292
>1k	0.07505193308117475	26.15735444998432
>5k	0.012104068604767749	13.414457727925708
>10k+	0.005470420183232212	19.06733938265981
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	883083	3.920458662094086	TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC	69949	0.310539510957429	TruSeq Adapter, Index 1 (98% over 50bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	66137	0.29361608652291643	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	51123	0.22696123488079373	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	43130	0.19147620562972897	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	40469	0.17966266092347558	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	39411	0.17496565592564917	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	38918	0.1727769759030325	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	34733	0.15419761303355844	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	34475	0.15305221862009985	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	34341	0.15245732384721822	No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT	33339	0.14800893159029757	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	32644	0.14492346989512805	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	31504	0.13986242481240393	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	31363	0.1392364534469091	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	30318	0.13459716212107867	No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA	29672	0.13172923657420166	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	29426	0.13063711631950856	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	29101	0.12919427451960916	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	29045	0.1289456617787034	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	28821	0.12795121081508043	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	28528	0.1266504334385557	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	27946	0.12406663673842815	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	26875	0.11931191806860575	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	26644	0.11828639051236955	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	25780	0.11445065108125234	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	25255	0.11211990663526096	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	25221	0.11196896318542533	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	24837	0.11026419010492879	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	24293	0.10784909490755869	No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG	23293	0.10340958167709892	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	23227	0.10311657380388858	No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT	22911	0.1017136876230633	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.011569371478578106	4.439513230459749E-6	0.0
2	0.011800226166562013	4.439513230459749E-6	4.439513230459749E-6
3	0.012839072262489594	4.439513230459749E-6	4.439513230459749E-6
4	0.01325638650615281	4.439513230459749E-6	8.879026460919497E-6
5	0.014819095163274641	4.439513230459749E-6	8.879026460919497E-6
6	0.015307441618625213	1.3318539691379247E-5	8.879026460919497E-6
7	0.016208662804408542	1.3318539691379247E-5	8.879026460919497E-6
8	0.01680799709052061	1.3318539691379247E-5	8.879026460919497E-6
9	0.01745616602216773	1.3318539691379247E-5	8.879026460919497E-6
10-11	0.019851283410000767	1.3318539691379247E-5	1.1098783076149373E-5
12-13	0.022204225422144435	1.3318539691379247E-5	1.3318539691379247E-5
14-15	0.024162050756777183	1.3318539691379247E-5	1.3318539691379247E-5
16-17	0.025540519614834938	1.3318539691379247E-5	2.2197566152298746E-5
18-19	0.02634185175293292	1.3318539691379247E-5	2.2197566152298746E-5
20-21	0.027078810949189236	1.3318539691379247E-5	2.6637079382758494E-5
22-23	0.027660387182379467	1.3318539691379247E-5	2.6637079382758494E-5
24-25	0.028202007796495555	1.3318539691379247E-5	2.6637079382758494E-5
26-27	0.028932307722906184	1.3318539691379247E-5	2.8856835997988368E-5
28-29	0.030625982020326577	1.3318539691379247E-5	3.107659261321824E-5
30-31	0.03481466275326535	1.3318539691379247E-5	3.107659261321824E-5
32-33	0.0370699354743389	1.3318539691379247E-5	3.107659261321824E-5
34-35	0.03926749452341648	1.3318539691379247E-5	3.3296349228448115E-5
36-37	0.0413496262285021	1.3318539691379247E-5	3.551610584367799E-5
38-39	0.04410212443138715	1.3318539691379247E-5	3.551610584367799E-5
40-41	0.04766705355544633	1.3318539691379247E-5	3.551610584367799E-5
42-43	0.052392915389270725	1.3318539691379247E-5	3.773586245890786E-5
44-45	0.05718980943478248	1.3318539691379247E-5	3.995561907413774E-5
46-47	0.06277027756547039	1.3318539691379247E-5	3.995561907413774E-5
48-49	0.06945396473392754	1.553829630660912E-5	3.995561907413774E-5
50-51	0.07758493321551457	1.7758052921838995E-5	3.995561907413774E-5
52-53	0.08757161822743378	1.7758052921838995E-5	3.995561907413774E-5
54-55	0.10032633973854463	1.7758052921838995E-5	3.995561907413774E-5
56-57	0.11274587800075578	1.7758052921838995E-5	3.995561907413774E-5
58-59	0.12715209843359768	1.7758052921838995E-5	4.6614888919827366E-5
60-61	0.1447481091225249	1.9977809537068872E-5	6.215318522643648E-5
62-63	0.16832192437626614	2.2197566152298746E-5	6.215318522643648E-5
64-65	0.19905889422735423	2.2197566152298746E-5	6.215318522643648E-5
66-67	0.23259275741363195	2.2197566152298746E-5	6.659269845689623E-5
68-69	0.2685838911729691	2.2197566152298746E-5	7.991123814827547E-5
70-71	0.315001221754041	2.2197566152298746E-5	8.879026460919498E-5
72-73	0.37823542845209446	2.2197566152298746E-5	8.879026460919498E-5
74-75	0.4494341218855927	2.2197566152298746E-5	8.879026460919498E-5
76-77	0.5193697738050251	2.2197566152298746E-5	8.879026460919498E-5
78-79	0.602905874505971	2.2197566152298746E-5	8.879026460919498E-5
80-81	0.7062244461618454	2.2197566152298746E-5	8.879026460919498E-5
82-83	0.8279692174807431	2.2197566152298746E-5	9.101002122442486E-5
84-85	0.9644265356453845	2.2197566152298746E-5	9.322977783965473E-5
86-87	1.1155786426028476	2.2197566152298746E-5	9.322977783965473E-5
88-89	1.2847507337627468	2.2197566152298746E-5	9.322977783965473E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGAGCA	141135	0.0	70.76785	7
CGGAAGA	148085	0.0	67.55411	4
AGAGCAC	148135	0.0	67.40134	8
TCGGAAG	148615	0.0	67.33916	3
GAGCACA	151625	0.0	66.037865	9
GATCGGA	153475	0.0	65.2226	1
GAAGAGC	164660	0.0	60.717808	6
GGAAGAG	168580	0.0	59.366623	5
ATCGGAA	178500	0.0	56.10495	2
GTCCAAT	6960	0.0	45.32204	1
TGCCGTC	108855	0.0	45.23305	48-49
ATGCCGT	109590	0.0	45.226757	46-47
GTATGCC	109810	0.0	45.224327	44-45
GCCGTCT	109555	0.0	45.219517	48-49
TATGCCG	109385	0.0	45.09644	46-47
CGTATGC	109795	0.0	44.996784	44-45
CTCGTAT	100865	0.0	44.933136	42-43
TCGTATG	111005	0.0	44.758522	42-43
TCCAATC	7060	0.0	44.738632	2
CCGTCTT	110360	0.0	44.663067	50-51
>>END_MODULE