FastQCFastQC Report
Wed 8 Oct 2014
Complete_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86667849
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGTTCTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTC1640120.1892420336865635No Hit
ACTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCAT1544330.1781894921610435No Hit
CGATGTTCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCAT1522520.1756729880304287No Hit
GATCAGTAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA1511590.17441185138908893No Hit
GATCAGTAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATAC1376810.15886052508352894No Hit
GATCAGTCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCAT1279730.1476591394347401No Hit
TTCGAGTCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCAT1235450.14254997836625666No Hit
CGATGTTGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACG1157730.13358240839691313No Hit
CGATGTTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTG1129780.13035745239275526No Hit
GATCAGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTG1113850.12851940054494718No Hit
TGACCATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCAT1025660.118343770133259No Hit
CGATGTTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACC1012390.11681263717529208No Hit
CGATGTTACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTT988490.11405498248837351No Hit
CGATGTTAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA980570.11314114880132771No Hit
TTCGAGTAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA964360.11127078970195742No Hit
CGATGTTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTT895280.10330012920939113No Hit
CGATGTTAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATAC894690.10323205321502787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGT15575550.044.8337631
TCGAGTC5528600.044.717222
GATCAGT12960150.044.5875171
CGATGTT14072750.044.579671
ACTTGAT13829700.044.5309521
TCGAGTA6111300.044.4908872
CTTGATC5671150.044.2822952
CGAGTCA2478600.044.2787063
TGACCAT7518150.044.275031
CGAGTCG1046350.044.2287523
TCGAGTG2564500.044.171322
ACAGTGT3849900.044.135111
ATCAGTC4753750.043.8979872
CTTGATA4466100.043.8721052
CGAGTAA2349300.043.8435553
CGAGTAG2401900.043.831383
CGAGTCC826500.043.4142073
GACCATA2519950.043.3523372
GATGTTC4855300.043.269922
CGAGTCT1289700.043.2330743
Produced by FastQC (version 0.11.2)