FastQCFastQC Report
Wed 8 Oct 2014
Complete_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86667849
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGNCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC3251150.3751275747018944No Hit
ACTTGANCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC2045720.23604139523527345No Hit
GATCAGNCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC1652610.19068316787232137No Hit
TTCGAGNCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG1608960.18564669811985296No Hit
TTCGAGNGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTT1300350.15003833774621542No Hit
CGATGTNATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCC1282000.1479210589384767No Hit
CGATGTNCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC1085340.12522983003766483No Hit
CGATGTNCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG1036210.11956106121890712No Hit
TGACCANCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC982400.1133522997668951No Hit
GATCAGNCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG978060.1128515373676806No Hit
ACTTGANCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG942140.1087069785244122No Hit
CGATGTNGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCG897750.10358512532138647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGA245750.033.65931724
TAAGCCG250900.033.0288323
TCCAGGG251550.032.94110511
CTCGGGG226600.032.8566723
AGCCGAT254700.032.46163625
CCTCGGG229950.032.37830422
GCCCCGT103850.032.3530548
ACATCCC261350.032.2167141
CAGGCGG268650.032.12179643
TCGGGGA270250.032.08413724
CCAGGGA263350.031.43636712
CCCAGGC275250.031.40440441
CCAGGCG279250.030.88651842
CGGGGAA284750.030.5370925
GGCGGTC284750.030.44036745
CGCGCGC41100.030.4073098
CATCCCT274900.030.27619642
CAGGGAT276300.029.98356213
TCCCAGG290050.029.87403940
GCCGATG277350.029.8311826
Produced by FastQC (version 0.11.2)