FastQCFastQC Report
Tue 19 Aug 2014
Complete_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86667849
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC3248600.3748333479465955No Hit
ACTTGATCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC2044690.235922550702741No Hit
GATCAGTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC1651340.19053663140987842No Hit
TTCGAGTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG1605260.1852197808670664No Hit
TTCGAGTGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTT1299060.14988949362294662No Hit
CGATGTTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCC1281650.14788067487402393No Hit
CGATGTTCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC1084550.12513867743504284No Hit
CGATGTTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGG1035830.11951721566321556No Hit
TGACCATCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATC981690.11327037780757661No Hit
GATCAGTCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG977200.11275230795216805No Hit
ACTTGATCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCG941310.10861121060013847No Hit
CGATGTTGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCG894340.10319166915057508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGT15589950.044.9794541
TCGAGTG5291350.044.821452
TCGAGTC5589650.044.7798842
CGATGTT14168800.044.7755851
CGAGTGC1615250.044.6773343
GATCAGT10301800.044.6378941
CGAGTCC2114500.044.587283
CGAGTCG1035450.044.562933
GAGTCCC1365700.044.453834
AGTCGCG305300.044.3879935
AGTCCCC1023050.044.383025
TCGAGTA2699500.044.3276862
ATCAGTC4932100.044.293912
CGAGTAG1216850.044.260763
CGAGTCT2053200.044.2592163
ACTTGAT13779000.044.138711
CGAGTGT1756050.044.1130683
CAGTCGC556000.044.0929874
GACCATG3106500.043.960682
TCAGTCG1180850.043.89633
Produced by FastQC (version 0.11.2)