FastQCFastQC Report
Tue 19 Aug 2014
lane1_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737944
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG23050.040.87464537
GGCGGGT10250.040.56445713
CGGGTAG9300.040.4010515
TCGGGGG41700.040.33940538
GCGGGTA7800.040.10370614
GGGCCGG15300.040.06135645
GATCGGG20650.039.737781
GGGCGTG6800.039.33107812
ACTCGGG8800.039.16885836
TAGGGGA21950.038.94954719
AGGGGCG16050.038.82446310
GTAGGGG22600.038.7263118
TCTCGGG15000.038.5988236
GCGCCGG9250.038.59311345
ATCGGGA21650.037.974312
GAGCGGC13150.037.636519
GGGCGCC13750.037.5469943
GATCTCG14450.037.3923834
ATCGGGG9300.036.83912737
CGGCGGG11250.035.3605112
Produced by FastQC (version 0.11.2)