FastQCFastQC Report
Tue 19 Aug 2014
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3737944
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC191030.5110563454134145TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC132430.3542856714814347TruSeq Adapter, Index 11 (100% over 51bp)
AGCTTCACCCTTAGAATAAGCTCATTTAGTATCTTTTCAGTCCCATACTAG49620.13274677202226678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG108550.042.63735611
CACACGT109100.042.3229512
ACACGTC108650.042.22965213
CACGTCT109400.041.8375514
AGCACAC111250.041.82843410
ACGTCTG108750.041.81927515
GTCACGG20000.041.74951629
AGAGCAC113350.041.5514648
GATCGGA118950.041.3492131
CGGAAGA117050.041.3021134
TCGGAAG118200.041.291583
CGTCTGA111550.040.72933216
ATCGGAA120950.040.7006072
ACGGCTA16750.040.21893332
TCACGGC17350.039.4784430
CACGGCT17200.039.4286831
GAACTCC115100.038.4954221
GAAGAGC123800.038.4317786
CTGAACT116900.038.37467619
CCCTTAG8150.038.251268
Produced by FastQC (version 0.11.2)