FastQCFastQC Report
Tue 19 Aug 2014
MRP13_GTAGAG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP13_GTAGAG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15587768
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC11258377.2225670795203TruSeq Adapter, Index 3 (97% over 37bp)
AGCTTCACCCTTAGAATAAGCTCATTTAGTATCTTTTCAGTCCCATACTAG470520.301852067595566No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATATCGTATGCC306610.1966991040667272TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1278200.043.33824511
GATCGGA1300850.043.3180771
ACACGTC1278750.043.20002413
CACACGT1281600.043.18115612
ACGTCTG1277950.043.10740315
TCGGAAG1304500.043.0604323
AGCACAC1290850.042.99542210
CACGTCT1283400.042.96639614
CGGAAGA1303600.042.9457784
GTATGCC1256850.042.8834845
CGTCTGA1286000.042.78335616
AGAGCAC1301300.042.765528
AGTCACG1275600.042.60965328
ATCGGAA1322000.042.6088942
GAAGAGC1311450.042.537226
GTCACGT1277450.042.47049329
GAACTCC1290850.042.40558221
CGTAGAG1274400.042.3939133
GAGCACA1312750.042.3187189
ACTCCAG1288900.042.3005523