FastQCFastQC Report
Tue 19 Aug 2014
MRP11_ATGTCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMRP11_ATGTCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8821099
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG4734835.3676191594720795TruSeq Adapter, Index 15 (97% over 40bp)
AGCTTCACCCTTAGAATAAGCTCATTTAGTATCTTTTCAGTCCCATACTAG203390.23057217700424856No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTATG110800.12560793161940478TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC552600.043.4111213
GCACACG554000.043.33794811
GATCGGA566850.043.144191
CACACGT556200.043.1382312
CACGTCT556300.043.05771614
ACGTCTG557400.043.04535715
TCGTATG550650.043.0358645
CGTCTGA560000.042.80936416
AGCACAC561550.042.80732710
TCGGAAG572100.042.7168923
CGGAAGA571100.042.659344
GAACTCC559200.042.45112621
AGAGCAC569500.042.4405568
ACTCCAG558050.042.3604223
CAGTCAC558750.042.33978727
CTCGTAT547050.042.2660644
CCAGTCA557950.042.259226
ATCGGAA580600.042.188422
AGTCACA564300.042.13854228
ATGTCAG563100.042.12284534