FastQCFastQC Report
Tue 19 Aug 2014
LW246_GCCAAT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW246_GCCAAT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6142771
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGG18700.038.78302845
TCGGGGG51400.038.47315638
TAGGGGA24600.037.57719
CTCGGGG30150.036.52696637
GGGGCCG23550.035.48219344
AGGGGCG22200.035.45672610
CGGGTAG15500.035.2597615
GCGGGTA12450.035.2268314
GGCGGGT17550.035.11211813
GATCGGG31900.034.223441
AGGGGAG37500.034.12936428
GTAGGGG28000.034.0583218
GGGGGCC30050.034.03540443
GGGGAAG47250.033.93876321
GAGGGGC16000.033.5942549
CGGGGGG60000.033.04577339
GGGCGCC17250.033.0408343
GGGGCGC15900.032.71738442
AGGGGAA55050.032.44088420
AGCGGCG24100.032.2881110
Produced by FastQC (version 0.11.2)