FastQCFastQC Report
Tue 19 Aug 2014
LW246_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW246_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6142771
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC705661.148764946634019TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC86700.041.474529
GCACACG89350.041.22834811
ACGTCTG89100.040.8392115
AGTCACG89550.040.40566628
CACGCCA88950.040.37253631
TCACGCC88850.040.36735530
GTATGCC89750.040.2384945
CGTCTGA90300.040.19687716
CGTATGC89600.040.1833144
CACACGT91850.039.93478412
ACACGTC91500.039.792613
CACGTCT91500.039.7680214
CTCGTAT90100.039.63616642
TCGTATG91950.039.2538443
CCAGTCA92450.038.82167426
ACTCCAG92500.038.80068623
CAGTCAC93950.038.4415227
GATCGGA97300.038.4021221
AGCACAC96500.038.31311410
ACGCCAA94350.038.13336632
Produced by FastQC (version 0.11.2)