FastQCFastQC Report
Tue 19 Aug 2014
LW245_GTTTCG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW245_GTTTCG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6365940
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGG38350.041.4113345
TCGGGGG78700.040.78680838
CTCGGGG47400.039.81921837
AGGGGCG32550.039.65815410
AGCGGCG42200.039.4891110
TAGGGGA45850.039.0459519
CGGGTAG23050.038.93116415
GTAGGGG47050.038.8149218
GGCGGGT24550.038.6567813
GAGCGGC33400.038.6488889
GCGGGTA20350.038.35047514
AGAGCGG34700.038.2379078
GATCGGG46500.038.1545751
GGGGCCG46400.038.11071444
GAGGGGC25450.037.908899
GGGGAAG73650.037.83532321
GGGGGCC54250.037.33564843
GCGGCGT20600.037.1178911
CGGGAGA46600.036.9638254
AGGGTAG26300.036.68715328
Produced by FastQC (version 0.11.2)