FastQCFastQC Report
Tue 19 Aug 2014
LW245_GTTTCG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW245_GTTTCG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6365940
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC1088751.710273738049683TruSeq Adapter, Index 21 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATATCGTATGCC96160.15105388992042024TruSeq Adapter, Index 21 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC134600.041.58107845
GCACACG140350.041.4970211
ACACGTC140800.041.25259413
AGTCACG136900.041.24514428
ACGTCTG140500.041.19663615
CACGTCT141150.041.10251214
CACACGT142050.040.9687412
CGTATGC137100.040.82670244
CGTCTGA142350.040.67703616
GTCACGT139300.040.61524229
TCGTATG138600.040.4809343
CCAGTCA141150.040.2259826
CAGTCAC141200.040.18020227
CGATCTC126600.039.91377638
ACTCCAG143150.039.8838923
GATCGGA148950.039.8117071
CTCGTAT128500.039.7086242
AGCACAC147300.039.63067610
GAACTCC145100.039.5000721
TCCAGTC144200.039.4375325
Produced by FastQC (version 0.11.2)