##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane2_Undetermined_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3750802	
Filtered Sequences	0	
Sequence length	51	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.880559144417646	34.0	31.0	34.0	31.0	34.0
2	33.03928733108279	34.0	33.0	34.0	31.0	34.0
3	33.07068514946937	34.0	33.0	34.0	31.0	34.0
4	36.388797115923474	37.0	37.0	37.0	35.0	37.0
5	36.307168173633265	37.0	37.0	37.0	35.0	37.0
6	36.31498943425966	37.0	37.0	37.0	35.0	37.0
7	36.30124010811554	37.0	37.0	37.0	35.0	37.0
8	36.31149738109343	37.0	37.0	37.0	35.0	37.0
9	38.109382740011334	39.0	39.0	39.0	37.0	39.0
10	38.09304810011299	39.0	39.0	39.0	37.0	39.0
11	38.09862317445709	39.0	39.0	39.0	37.0	39.0
12	38.094674685573914	39.0	39.0	39.0	37.0	39.0
13	38.084182796106006	39.0	39.0	39.0	37.0	39.0
14	39.657647351153166	41.0	40.0	41.0	37.0	41.0
15	39.63237328976576	41.0	40.0	41.0	37.0	41.0
16	39.618073148089394	41.0	40.0	41.0	37.0	41.0
17	39.58880394113046	41.0	40.0	41.0	37.0	41.0
18	39.56340137389284	41.0	40.0	41.0	37.0	41.0
19	39.60817606474562	41.0	40.0	41.0	37.0	41.0
20	39.57190355555959	41.0	40.0	41.0	37.0	41.0
21	39.572524222819546	41.0	40.0	41.0	37.0	41.0
22	39.48753813184487	41.0	40.0	41.0	37.0	41.0
23	39.47778928346524	41.0	40.0	41.0	37.0	41.0
24	39.483754407724	41.0	40.0	41.0	37.0	41.0
25	39.418725915150944	41.0	40.0	41.0	36.0	41.0
26	39.21599727205008	41.0	40.0	41.0	36.0	41.0
27	39.137608170199336	41.0	39.0	41.0	36.0	41.0
28	39.043323001320786	41.0	39.0	41.0	36.0	41.0
29	38.79762861382712	41.0	39.0	41.0	35.0	41.0
30	38.72754706860026	41.0	39.0	41.0	35.0	41.0
31	38.55360400255732	41.0	39.0	41.0	35.0	41.0
32	38.40866673314134	41.0	39.0	41.0	35.0	41.0
33	38.25160885591935	41.0	39.0	41.0	34.0	41.0
34	38.256783749182176	41.0	39.0	41.0	34.0	41.0
35	37.99313213547396	40.0	38.0	41.0	34.0	41.0
36	37.877400086701456	40.0	38.0	41.0	34.0	41.0
37	37.67528864493514	40.0	38.0	41.0	33.0	41.0
38	37.574491002190996	40.0	38.0	41.0	33.0	41.0
39	37.252535857664576	40.0	38.0	41.0	32.0	41.0
40	37.1067806298493	40.0	38.0	41.0	31.0	41.0
41	37.041589505391116	40.0	38.0	41.0	31.0	41.0
42	37.01264556220243	40.0	38.0	41.0	31.0	41.0
43	36.89233795865524	40.0	38.0	41.0	31.0	41.0
44	36.69287768322615	40.0	37.0	41.0	30.0	41.0
45	36.772984017818054	40.0	38.0	41.0	31.0	41.0
46	36.69060083683436	40.0	37.0	41.0	30.0	41.0
47	36.56935263444991	40.0	37.0	41.0	30.0	41.0
48	36.399186094067346	40.0	37.0	41.0	30.0	41.0
49	36.361191819776145	40.0	37.0	41.0	29.0	41.0
50	36.25376892728542	40.0	37.0	41.0	29.0	41.0
51	36.12348505732907	40.0	36.0	41.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	24.0
14	66.0
15	234.0
16	645.0
17	1425.0
18	3689.0
19	11167.0
20	11969.0
21	11889.0
22	16118.0
23	18767.0
24	17668.0
25	19219.0
26	24741.0
27	30044.0
28	38556.0
29	40465.0
30	44060.0
31	46010.0
32	52897.0
33	60022.0
34	72081.0
35	97017.0
36	147119.0
37	233825.0
38	492024.0
39	2251258.0
40	7800.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.60846573543897	24.6973456650876	28.278508649133222	26.41567995034021
2	22.086250395584702	27.8457503605253	26.149408509223555	23.918590734666445
3	22.361149621414096	25.48685613735736	28.752426149088194	23.399568092140342
4	22.0770917793048	24.648728458606985	27.1933575805921	26.080822181496117
5	24.137675379187613	26.418601574915705	27.175283312278097	22.268439733618585
6	24.62795956704726	26.649527221111647	25.56655883195114	23.155954379889955
7	22.91016694562923	28.524086315406677	26.392568842610192	22.1731778963539
8	22.607564995432977	28.780911389084256	25.826583221401716	22.78494039408105
9	24.08130314530066	26.456981733506595	26.486308794759093	22.975406326433653
10	22.4166458266792	28.596604139594678	25.686293224755662	23.300456808970456
11	24.020489484648884	26.602044042847368	26.06370584211057	23.31376063039318
12	22.3384758779589	26.61084216122312	25.975777980282615	25.074903980535364
13	22.141104755729575	28.823515610794704	25.98353632103214	23.05184331244358
14	22.2067707119704	26.965353009836296	25.958528336073194	24.86934794212011
15	22.1910940646827	28.43178605535563	26.19826373132999	23.17885614863168
16	22.37966706853628	26.842605928012198	25.962234210176916	24.81549279327461
17	24.16413876285658	26.91738993420607	25.90086066926487	23.01761063367248
18	22.400945971208575	26.77523609944752	27.616694983755117	23.207122945588786
19	22.35173326891996	26.807880186862764	26.005832396193423	24.834554148023848
20	22.509819231213328	26.790014717180277	27.582726472978013	23.11743957862838
21	24.098019570214582	26.756437689859396	25.971245616270867	23.174297123655155
22	22.465499940872675	28.501638881273667	25.932176288581914	23.10068488927174
23	22.43124121438852	28.50017027851805	26.005516650625548	23.063071856467875
24	22.431833688250254	26.78586045112798	26.022844616243134	24.75946124437863
25	22.532835294224416	26.85983921743016	27.580739616904214	23.026585871441206
26	22.560012943048978	26.95829241754833	25.820659484774506	24.661035154628188
27	22.534758186625353	26.81363044294038	25.93291382712498	24.71869754330929
28	22.66631728633399	28.54105639095123	25.71847812501844	23.074148197696342
29	24.244233850152558	26.993970817168396	25.7774753097097	22.984320022969342
30	22.61208287843547	26.931284171280375	27.297890496554285	23.158742453729875
31	22.43102128634596	27.15811545663781	25.858411700692212	24.552451556324016
32	22.697255149901025	28.408513363353787	25.829947100140693	23.06428438660449
33	22.660849750905797	27.07149373780658	25.89346759162486	24.374188919662764
34	22.881875140148892	27.298259300003437	26.040712899054114	23.779152660793553
35	22.994190507867692	27.648693123695704	25.938369246011266	23.418747122425337
36	23.123352969856665	27.64051899924555	25.94657148380855	23.289556547089234
37	22.736309768802105	27.920142126836	26.11852621988446	23.225021884477435
38	22.88304490076469	27.436320194957137	26.48576162806313	23.19487327621504
39	22.76092725932583	27.668399430700134	26.202430148987453	23.36824316098658
40	22.733899417655476	27.75771982958124	26.3907812135584	23.117599539204882
41	22.65861604747032	27.22472792034198	26.730453222745837	23.386202809441865
42	22.848247386492275	26.893644406202107	26.555562117342784	23.70254608996284
43	22.818353097639367	27.081126011304445	26.57270309209797	23.527817798958218
44	23.314935757112977	27.122913551828354	26.0600529214803	23.50209776957837
45	23.40625328477146	27.06891460974336	26.460627213256355	23.064204892228826
46	22.933122018596517	27.627760848854205	26.51424668217029	22.924870450378982
47	22.81857164121409	27.6822058260004	26.54772319980669	22.951499332978823
48	23.212561200197637	27.13642029376095	26.50224026411535	23.148778241926067
49	23.386046464679886	27.10141518229027	25.933952178261432	23.57858617476841
50	22.965231158979208	27.001601873011218	25.88955779911042	24.143609168899157
51	23.247850923378444	26.958769666131655	25.997525566006356	23.795853844483542
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.02414568577918
2	46.004841130251144
3	45.76071771355444
4	48.15791396080092
5	46.4061151128062
6	47.78391394693722
7	45.083344841983134
8	45.392505389514035
9	47.05670947173431
10	45.71710263564965
11	47.33425011504207
12	47.41337985849427
13	45.19294806817315
14	47.07611865409051
15	45.36995021331438
16	47.195159861810886
17	47.18174939652906
18	45.608068916797365
19	47.18628741694381
20	45.627258809841706
21	47.27231669386974
22	45.566184830144415
23	45.49431307085639
24	47.191294932628885
25	45.559421165665626
26	47.22104809767716
27	47.25345572993464
28	45.74046548403034
29	47.2285538731219
30	45.770825332165344
31	46.983472842669975
32	45.761539536505516
33	47.03503867056856
34	46.66102780094245
35	46.41293763029303
36	46.4129095169459
37	45.961331653279544
38	46.07791817697973
39	46.129170420312406
40	45.85149895686036
41	46.04481885691219
42	46.55079347645511
43	46.346170896597584
44	46.81703352669135
45	46.47045817700028
46	45.8579924689755
47	45.77007097419291
48	46.36133944212371
49	46.9646326394483
50	47.10884032787837
51	47.04370476786199
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	46.5
2	57.0
3	88.0
4	119.0
5	180.0
6	241.0
7	402.0
8	563.0
9	849.5
10	1136.0
11	1682.5
12	2229.0
13	3088.0
14	3947.0
15	5385.0
16	6823.0
17	8752.0
18	10681.0
19	13324.5
20	15968.0
21	19376.0
22	22784.0
23	27005.5
24	31227.0
25	37614.5
26	52429.5
27	60857.0
28	68673.5
29	76490.0
30	89617.5
31	102745.0
32	118891.0
33	135037.0
34	154110.0
35	173183.0
36	191020.5
37	208858.0
38	221853.0
39	234848.0
40	245947.5
41	257047.0
42	274954.5
43	292862.0
44	299702.0
45	306542.0
46	298702.5
47	290863.0
48	279312.0
49	267761.0
50	257422.5
51	247084.0
52	238989.0
53	230894.0
54	197889.0
55	164884.0
56	142223.0
57	119562.0
58	104112.0
59	88662.0
60	80156.0
61	71650.0
62	62892.5
63	54135.0
64	48677.0
65	43219.0
66	39554.0
67	35889.0
68	32426.5
69	28964.0
70	27025.5
71	25087.0
72	23321.0
73	21555.0
74	20135.5
75	17147.5
76	15579.0
77	14120.5
78	12662.0
79	10936.0
80	9210.0
81	7923.0
82	6636.0
83	5482.0
84	4328.0
85	3446.5
86	2565.0
87	1956.5
88	1348.0
89	994.0
90	640.0
91	443.0
92	246.0
93	181.5
94	117.0
95	78.5
96	40.0
97	38.0
98	36.0
99	112.0
100	188.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.01290390695110006
2	6.66524119375003E-4
3	5.3321929550000246E-5
4	0.0
5	0.030420160808275136
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.02679426959887512
19	7.465070137000034E-4
20	0.020342316123325092
21	0.0
22	0.12394682523897557
23	0.02834060555582513
24	0.004239093399225019
25	0.11666838185540053
26	0.4672067467171021
27	0.46296765331787715
28	0.5944328706234027
29	1.1995567881215803
30	1.1466880949727551
31	1.561692672660407
32	1.7472796484591828
33	2.044629388594759
34	1.4354530044507814
35	2.369199973765611
36	2.5018915954507865
37	2.9656590777119134
38	2.7194184070500125
39	3.640234808448967
40	3.8621340182712927
41	3.771806669613592
42	3.3893551299162152
43	3.775245934069567
44	4.331526964099944
45	4.377063891935644
46	4.684971374122121
47	4.890927327008996
48	5.031510594267573
49	4.9126826742653975
50	5.3477362974638485
51	5.334619102794549
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3750802.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	34.56498765856227
#Duplication Level	Relative count
1	100.0
2	5.2946648176998625
3	1.0466065181786535
4	0.432566925179618
5	0.23911021325891813
6	0.16948862371813528
7	0.12212311607973383
8	0.09244834020989197
9	0.09244834020989197
10++	6.6442964510109395
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC	7543	0.2011036572978259	TruSeq Adapter, Index 7 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	426160	2.5233467	20.718317	12
GGAAG	348940	2.2874384	23.842667	5
CTGAA	375895	2.0647104	17.980726	19
TCCAG	305430	1.9345102	18.578587	25
AAGAG	344930	1.8955334	19.714172	7
CTCCA	305840	1.8725015	18.516493	24
AGAGC	289570	1.8349353	22.255947	8
GAAGA	323885	1.7798824	19.683752	6
TCTGA	308335	1.7512133	18.314362	18
CACCA	294170	1.7418175	6.2068	31
ACCAG	278385	1.7052246	5.404847	32
GTCTG	246455	1.6697524	21.530214	17
AGCAC	272325	1.6681043	20.947151	10
GAGCA	262855	1.6656487	21.833567	9
GCACA	268450	1.6443685	20.667423	11
CAGTC	253300	1.604333	17.254549	27
CCAGT	244210	1.5467595	17.866793	26
ATGCC	233480	1.4787985	8.631361	47
AGTCA	266025	1.4612179	14.284152	28
ATCTC	249400	1.3692449	7.6405954	40
TCACC	220215	1.3482634	7.877709	30
TGAAC	234500	1.2880579	17.02452	20
ACTCC	207910	1.2729262	18.129366	23
AACTC	238870	1.2683021	16.196177	22
GAACT	229220	1.259056	16.841673	21
GTCAC	198320	1.2561047	14.974693	29
GTATG	212965	1.251284	7.7485104	45
CACGT	183230	1.1605288	20.250515	14
TATGC	199090	1.1307474	7.4485235	46
TGCCG	144665	1.0930021	8.464666	47
CGGAA	167685	1.0625794	22.019733	4
TCTCG	146170	0.9572849	8.476314	41
ACGTC	150710	0.954556	19.791862	15
CGTCT	143940	0.9426803	20.22429	16
CTCGT	135710	0.88878113	8.295182	42
ACACG	133690	0.8189071	19.383215	13
ATCGG	120600	0.79020196	22.51775	2
TCGGA	118990	0.7796529	22.504026	3
CGTAT	135815	0.7713721	7.0522876	44
GATCG	94620	0.6199744	22.298536	1
TCGTA	107275	0.60927695	6.963906	43
>>END_MODULE