##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	lane1_Undetermined_L001_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4707422	
Filtered Sequences	0	
Sequence length	51	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65073749496008	34.0	31.0	34.0	31.0	34.0
2	32.81734014923667	34.0	31.0	34.0	31.0	34.0
3	32.8775814023047	34.0	31.0	34.0	31.0	34.0
4	36.129298371805206	37.0	37.0	37.0	35.0	37.0
5	36.11074320509187	37.0	37.0	37.0	35.0	37.0
6	36.13218339039925	37.0	37.0	37.0	35.0	37.0
7	36.11796605445613	37.0	37.0	37.0	35.0	37.0
8	36.086047522401856	37.0	37.0	37.0	35.0	37.0
9	37.86573627773333	39.0	38.0	39.0	35.0	39.0
10	37.86095361750019	39.0	38.0	39.0	35.0	39.0
11	37.887646359302394	39.0	38.0	39.0	35.0	39.0
12	37.89956328538211	39.0	38.0	39.0	35.0	39.0
13	37.885203833435796	39.0	38.0	39.0	35.0	39.0
14	39.406763404683076	41.0	40.0	41.0	36.0	41.0
15	39.36786015785285	41.0	39.0	41.0	36.0	41.0
16	39.37315392586431	41.0	39.0	41.0	36.0	41.0
17	39.31255918844752	41.0	39.0	41.0	36.0	41.0
18	39.301808718232614	41.0	39.0	41.0	36.0	41.0
19	39.343920515305406	41.0	39.0	41.0	36.0	41.0
20	39.31630072680971	41.0	39.0	41.0	36.0	41.0
21	39.30787679540946	41.0	39.0	41.0	36.0	41.0
22	39.25252994101655	41.0	39.0	41.0	36.0	41.0
23	39.24742566100936	41.0	39.0	41.0	36.0	41.0
24	39.21347416908873	41.0	39.0	41.0	36.0	41.0
25	39.14762347628915	41.0	39.0	41.0	36.0	41.0
26	39.00402428335509	41.0	39.0	41.0	36.0	41.0
27	38.92142748196359	41.0	39.0	41.0	35.0	41.0
28	38.77039662048569	41.0	39.0	41.0	35.0	41.0
29	38.643387612157994	40.0	39.0	41.0	35.0	41.0
30	38.567656139602526	40.0	39.0	41.0	35.0	41.0
31	38.48592605464307	40.0	39.0	41.0	34.0	41.0
32	38.30073976796641	40.0	38.0	41.0	34.0	41.0
33	38.328060666751355	40.0	38.0	41.0	34.0	41.0
34	37.85884524480703	40.0	38.0	41.0	33.0	41.0
35	37.734032767829184	40.0	38.0	41.0	33.0	41.0
36	37.58714600050728	40.0	38.0	41.0	32.0	41.0
37	37.31188365946372	40.0	38.0	41.0	31.0	41.0
38	37.220897127982155	40.0	38.0	41.0	31.0	41.0
39	37.02309820534467	40.0	38.0	41.0	30.0	41.0
40	37.12736121809347	40.0	37.0	41.0	30.0	41.0
41	37.04705462990146	40.0	37.0	41.0	31.0	41.0
42	37.02711590335432	40.0	37.0	41.0	31.0	41.0
43	36.85059210752722	40.0	37.0	41.0	30.0	41.0
44	36.74031518737857	40.0	37.0	41.0	30.0	41.0
45	36.82358178213043	40.0	37.0	41.0	31.0	41.0
46	36.81898839747106	40.0	37.0	41.0	30.0	41.0
47	36.70413487467238	40.0	37.0	41.0	30.0	41.0
48	36.65273986483472	40.0	37.0	41.0	30.0	41.0
49	36.57878537339546	40.0	37.0	41.0	30.0	41.0
50	36.51610393119631	40.0	36.0	41.0	30.0	41.0
51	36.2968813928303	40.0	36.0	41.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	24.0
14	95.0
15	276.0
16	705.0
17	1588.0
18	5431.0
19	8111.0
20	13566.0
21	12620.0
22	14426.0
23	15812.0
24	20697.0
25	23255.0
26	32177.0
27	41007.0
28	50497.0
29	54071.0
30	62891.0
31	69181.0
32	77936.0
33	91238.0
34	113288.0
35	161036.0
36	221135.0
37	342294.0
38	710270.0
39	2559067.0
40	4723.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.168233658756392	24.83515512318778	26.744778681797605	24.251832536258224
2	21.228896936446812	31.039991773985143	25.7273288523647	22.00378243720334
3	21.03467885515401	24.689334415036093	32.01746699287695	22.258519736932946
4	21.070898746908647	24.234615315712652	26.415560717646247	28.27892521973246
5	26.921067820362786	26.018089310473826	25.884831630737935	21.176011238425456
6	27.435867091623784	25.91655545926197	24.671195416038213	21.97638203307603
7	21.636067469625626	32.05295807344232	25.2821395659875	21.028834890944555
8	21.470092722581644	31.857519831585257	25.166272113052695	21.506115332780404
9	26.916346144450188	25.806609222627586	25.540561266867513	21.736483366054713
10	21.584064483702544	31.733547576571635	24.721684182977434	21.960703756748387
11	26.65405820850563	26.317738243990025	25.011906729415806	22.01629681808854
12	21.23928283128691	26.24548583081956	25.07324637804308	27.441984959850448
13	21.08693463216172	31.81235929134885	25.13316205770377	21.967544018785652
14	21.182995703380747	26.46093764272674	25.232218398945328	27.123848254947188
15	21.130175771271098	31.389869597554554	25.270197954323987	22.209756676850365
16	21.394980097386636	26.24315389612403	25.241799014407462	27.120066992081863
17	26.410973989584956	26.458537178098755	25.25250551150927	21.877983320807015
18	21.345759950988036	26.289230071151472	30.07648347651857	22.288526501341924
19	21.660263303353723	26.278119956103364	25.128743503344293	26.932873237198617
20	21.71585636469388	26.455414449777393	29.793632268362597	22.035096917166126
21	26.36122276694123	26.358503656566164	24.999458302229968	22.280815274262643
22	21.62172841100713	31.283088705452794	24.984439466017704	22.11074341752237
23	21.33507469693603	31.430706658548992	25.170847228058157	22.06337141645682
24	21.344136343431643	26.28410281113092	25.28829509274953	27.083465752687907
25	21.52833541790641	26.35991020496849	29.89445745929555	22.21729691782955
26	21.61311455851069	26.560986340244348	25.050200108016785	26.775698993228183
27	21.615748725630535	26.299072496041976	24.990408659318135	27.094770119009354
28	21.973436181422766	31.111611162077846	24.890913026819188	22.024039629680203
29	26.286870317506626	26.4863696519376	25.17945943027977	22.047300600275996
30	21.582166766868692	26.44134026401502	29.464321475225113	22.512171493891174
31	21.44589876825109	27.020539731614402	24.96875470610117	26.56480679403334
32	21.73291350170346	30.679242969129035	25.32688453590281	22.260958993264694
33	21.818913811976895	26.666999485559124	25.456660016716647	26.05742668574733
34	22.531411940086603	27.153384428463763	27.52840953838101	22.786794093068625
35	24.146606777462555	27.31545969233361	25.065273702573688	23.47265982763015
36	21.968506351462416	29.306731172458413	24.945334623794675	23.779427852284503
37	21.708788266476887	28.03933911059818	25.2096219692089	25.042250653716035
38	21.978469350074658	27.965096529529944	25.566853011624772	24.489581108770622
39	21.947100447555975	29.65125307614419	25.776318715436595	22.625327760863236
40	21.647979235865954	29.611455054518892	26.139223170964993	22.601342538650158
41	21.88041438551461	26.74856097967871	28.458462769744752	22.912561865061928
42	22.18356969666204	26.18474880808872	26.45950442896649	25.172177066282753
43	22.035132556694354	26.55497011019404	28.302364051709798	23.1075332814018
44	22.874507973216353	26.58481700753735	25.57513830327778	24.965536715968522
45	24.751641235259658	26.5763057616068	26.413330798069534	22.258722205064004
46	22.20800745934512	27.427439897038543	28.14419789410595	22.220354749510392
47	22.083210316383084	29.271627370594423	26.418558815972588	22.226603497049904
48	22.804806830564807	26.55744423737057	27.996793335485183	22.640955596579442
49	24.768991309002296	26.48471944417055	25.358904222951555	23.38738502387559
50	22.35873997144119	26.301523533669975	25.30728073259485	26.03245576229399
51	22.7303830777761	26.2392471135313	25.45021291451764	25.58015689417496
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	48.42006619501461
2	43.23267937365015
3	43.29319859208696
4	49.349823966641104
5	48.09707905878824
6	49.41224912469981
7	42.66490236057018
8	42.97620805536205
9	48.6528295105049
10	43.544768240450935
11	48.67035502659417
12	48.68126779113736
13	43.054478650947374
14	48.30684395832793
15	43.33993244812146
16	48.5150470894685
17	48.28895731039197
18	43.63428645232996
19	48.593136540552344
20	43.750953281860006
21	48.64203804120387
22	43.7324718285295
23	43.39844611339285
24	48.42760209611955
25	43.74563233573596
26	48.38881355173887
27	48.710518844639886
28	43.99747581110297
29	48.33417091778263
30	44.09433826075987
31	48.010705562284436
32	43.99387249496815
33	47.87634049772422
34	45.318206033155235
35	47.619266605092704
36	45.747934203746915
37	46.751038920192926
38	46.46805045884528
39	44.57242820841921
40	44.24932177451611
41	44.79297625057653
42	47.35574676294479
43	45.14266583809616
44	47.84004468918487
45	47.010363440323665
46	44.42836220885551
47	44.30981381343299
48	45.445762427144246
49	48.15637633287789
50	48.391195733735174
51	48.31053997195106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	97.0
2	113.0
3	184.5
4	256.0
5	417.5
6	579.0
7	784.5
8	990.0
9	1348.5
10	1707.0
11	2465.0
12	3223.0
13	4456.0
14	5689.0
15	7129.0
16	8569.0
17	10755.0
18	12941.0
19	16147.0
20	19353.0
21	23385.0
22	27417.0
23	32106.0
24	36795.0
25	44286.0
26	62334.0
27	72891.0
28	80906.0
29	88921.0
30	103552.0
31	118183.0
32	138297.5
33	158412.0
34	182965.0
35	207518.0
36	231698.5
37	255879.0
38	272914.0
39	289949.0
40	307043.0
41	324137.0
42	354034.0
43	383931.0
44	390592.0
45	397253.0
46	383162.5
47	369072.0
48	351608.5
49	334145.0
50	326483.5
51	318822.0
52	351803.0
53	384784.0
54	301783.5
55	218783.0
56	176246.0
57	133709.0
58	113464.5
59	93220.0
60	85205.5
61	77191.0
62	66880.0
63	56569.0
64	51891.0
65	47213.0
66	43213.5
67	39214.0
68	36073.0
69	32932.0
70	30602.0
71	28272.0
72	26756.5
73	25241.0
74	23418.0
75	19662.0
76	17729.0
77	15897.0
78	14065.0
79	12339.5
80	10614.0
81	8991.5
82	7369.0
83	6086.0
84	4803.0
85	3791.0
86	2779.0
87	2100.0
88	1421.0
89	1032.0
90	643.0
91	457.0
92	271.0
93	198.0
94	125.0
95	95.5
96	66.0
97	62.0
98	58.0
99	105.5
100	153.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008943323967980777
2	0.008582192121292716
3	6.160484443502197E-4
4	0.3938036572884267
5	2.1243049805179993E-5
6	0.015953530403690172
7	0.0
8	0.16136220632014722
9	0.0
10	0.0
11	0.0
12	4.6734709571395977E-4
13	0.0
14	0.0
15	0.004779686206165498
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.07012330740689915
25	0.08116969330559275
26	0.2911784836796021
27	0.33330345144327406
28	0.5926598465147166
29	0.8334923021560421
30	0.8183035215453385
31	0.9754808470538652
32	1.2645350257529493
33	0.854374220114534
34	1.5464727827672982
35	1.1458501064914088
36	1.625518171092373
37	2.333761451597074
38	2.318466455737344
39	2.4650647424428915
40	2.276872564218802
41	2.5881894591137145
42	2.4965894283537784
43	2.916713224350823
44	3.2565595351340924
45	3.36186133301837
46	3.3100070484439255
47	3.5128569310335895
48	3.386014680646859
49	3.481098571574845
50	3.6882183071753496
51	4.103498687816813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4707422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.90623250126369
#Duplication Level	Relative count
1	100.0
2	15.340365039847974
3	4.6786382452160336
4	2.319431963820102
5	1.9761943343694297
6	1.9865061944816818
7	2.0704741982528763
8	2.0189148976916163
9	1.8251992398685974
10++	15.096563204336874
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	66317	1.4087753339301214	TruSeq Adapter, Index 4 (100% over 51bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC	6461	0.1372513447912679	No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA	5834	0.12393195256342007	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATGCC	5651	0.12004447444907212	TruSeq Adapter, Index 4 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCATCACTGACCAATCTCGTATGCCG	5035	0.10695875576908125	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCAATCTCGTATGCCG	4871	0.10347489560103174	TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATATCTCGTATGCCG	4718	0.1002247089808392	TruSeq Adapter, Index 6 (97% over 37bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGAAG	592655	3.0697176	66.31335	5
CACAC	629670	2.8766398	53.89318	12
CTGAA	624180	2.6841857	47.87755	19
TCCAG	511900	2.5776725	51.34249	25
AGAGC	518355	2.5748334	61.138947	8
AAGAG	597750	2.5357208	53.063503	7
CTCCA	517700	2.500035	51.010277	24
GTCTG	445170	2.470806	61.35477	17
TCTGA	534150	2.4280713	50.521523	18
GAAGA	567315	2.4066126	53.7219	6
GAGCA	477755	2.3731604	59.975883	9
AGCAC	497825	2.371504	57.089436	10
CAGTC	468150	2.3573692	48.967026	27
GCACA	469765	2.2378337	55.954624	11
ATGCC	444245	2.236996	33.93915	47
CCAGT	443330	2.232388	50.179913	26
CACTG	425195	2.1410694	26.445932	31
ACCAA	532275	2.0766757	17.290148	36
ATCTC	474810	2.0698712	29.520054	40
GTATG	433915	2.056731	31.817629	45
AGTCA	477755	2.0545087	40.20707	28
ACTCC	416645	2.0120282	51.272095	23
CACGT	393050	1.9792032	56.77769	14
CGGAA	397820	1.976098	63.059574	4
TGAAC	458265	1.970695	46.809116	20
CTGAC	386230	1.9448613	21.693855	33
GTCAC	385910	1.9432497	44.10557	29
TATGC	416355	1.8926136	30.428024	46
GAACT	439435	1.8897196	46.552788	21
ACTGA	437905	1.8831398	19.691042	32
AACTC	452545	1.8663357	44.498676	22
GACCA	391735	1.8661199	19.98162	35
TCTCG	350390	1.8650469	35.36317	41
CGTCT	350340	1.8647808	58.721725	16
CTCGT	343335	1.8274947	35.255688	42
ACGTC	357590	1.8006444	55.869785	15
ATCGG	335540	1.7618202	66.58716	2
TCGGA	329670	1.7309986	66.58047	3
TGACC	337450	1.6992296	21.02003	34
AATCT	439300	1.6354783	19.702158	39
TCACT	371530	1.6196359	26.409721	30
CGTAT	350740	1.5943491	30.126942	44
ACACG	328835	1.5664812	53.786217	13
CCAAT	375545	1.5487812	19.391151	37
GATCG	293575	1.5414746	66.38361	1
CAATC	373510	1.5403883	20.013666	38
TGCCG	248840	1.5299505	16.200943	47
TCGTA	304995	1.3864075	29.887953	43
GCCGT	178015	1.094495	6.889492	47
TCACG	202860	1.0215015	8.090554	30
CACGC	149085	0.83161247	6.604468	31
>>END_MODULE