FastQCFastQC Report
Tue 12 Aug 2014
SV40_CAGATC_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SV40_CAGATC_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24496430
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC 1113539 4.54571951912993 TruSeq Adapter, Index 7 (100% over 51bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 3982965 3.950926 57.10507 12
GGAAG 2818030 3.2506382 65.58499 5
TCCAG 3011405 3.1316164 58.14411 25
GTGTG 2656470 3.0454733 3.4918354 4
CCAGA 2778030 2.89783 57.954174 33
CTCCA 2825305 2.7939663 55.127922 24
CTGAA 3399435 2.7705107 46.223118 19
AGAGC 2451290 2.688897 61.83807 8
GCACA 2507485 2.615618 58.611843 11
GAGCA 2377110 2.6075268 61.660732 9
GTCTG 2376835 2.5912263 60.792023 17
CACCA 2594135 2.5732677 54.936073 31
GAAGA 2978975 2.5609436 49.00372 6
AAGAG 2879595 2.4755096 48.851204 7
ACCAG 2367810 2.4699194 57.510498 32
CCAGT 2356570 2.4506412 57.35064 26
AGCAC 2298430 2.3975477 58.496635 10
TCTGA 2876895 2.3374403 45.692547 18
CAGTC 2235285 2.3245146 57.228455 27
ATGCC 2109585 2.1937969 56.992252 47
CATCT 2794170 2.1588666 42.755646 39
TCACC 2156755 2.132832 54.355015 30
ACTCC 2134440 2.1107645 54.582985 23
GTCAC 2020265 2.1009114 57.014877 29
CAGAT 2546820 2.0756366 45.085327 34
CACGT 1905425 1.981487 57.647797 14
AGTCA 2424060 1.9755883 45.006996 28
GAACT 2349925 1.9151689 45.225132 21
AACTC 2459645 1.9062594 43.109074 22
ATCTC 2382640 1.8409052 42.473866 40
TGAAC 2222510 1.8113269 45.159256 20
TCATC 2314175 1.7880069 42.409046 38
GTATG 2059995 1.760055 46.751083 45
AGATC 2132345 1.737843 44.680656 35
ACGTC 1639520 1.7049675 57.315453 15
CGGAA 1529050 1.677263 61.035007 4
TCTCG 1567605 1.6251725 56.15797 41
GATCA 1964015 1.6006556 44.548126 36
TATGC 1967520 1.5985848 44.38593 46
ATCAT 2520185 1.5260162 33.477165 37
ACACG 1461220 1.5242337 57.400684 13
TCGGA 1345330 1.4712001 60.708294 3
CGTCT 1403560 1.4551032 56.845524 16
CTCGT 1385260 1.4361311 55.924572 42
ATCGG 1306985 1.4292675 60.69754 2
GATCG 1299875 1.4214922 60.62434 1
CGTAT 1335435 1.0850238 43.88064 44
TCGTA 1296120 1.0530809 43.826187 43
Produced by FastQC (version 0.10.1)