##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV40_CAGATC_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	24496430	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.961480305497574	34.0	31.0	34.0	31.0	34.0
2	33.11281447949762	34.0	33.0	34.0	31.0	34.0
3	33.15835495212976	34.0	33.0	34.0	31.0	34.0
4	36.48887254183569	37.0	37.0	37.0	35.0	37.0
5	36.41850420653132	37.0	37.0	37.0	35.0	37.0
6	36.431655796375225	37.0	37.0	37.0	35.0	37.0
7	36.412118867933	37.0	37.0	37.0	35.0	37.0
8	36.429426124541415	37.0	37.0	37.0	35.0	37.0
9	38.251012576118235	39.0	39.0	39.0	37.0	39.0
10	38.237889765978146	39.0	39.0	39.0	37.0	39.0
11	38.240592363866895	39.0	39.0	39.0	37.0	39.0
12	38.2384556035308	39.0	39.0	39.0	37.0	39.0
13	38.23298721487172	39.0	39.0	39.0	37.0	39.0
14	39.82770109767015	41.0	40.0	41.0	38.0	41.0
15	39.80276950559735	41.0	40.0	41.0	38.0	41.0
16	39.808005656334416	41.0	40.0	41.0	38.0	41.0
17	39.77843502093979	41.0	40.0	41.0	38.0	41.0
18	39.77005588161214	41.0	40.0	41.0	38.0	41.0
19	39.819360045525	41.0	40.0	41.0	38.0	41.0
20	39.79046930511915	41.0	40.0	41.0	38.0	41.0
21	39.78962587609705	41.0	40.0	41.0	38.0	41.0
22	39.73524199240461	41.0	40.0	41.0	37.0	41.0
23	39.72624112982994	41.0	40.0	41.0	37.0	41.0
24	39.72873794263082	41.0	40.0	41.0	37.0	41.0
25	39.70232760447134	41.0	40.0	41.0	37.0	41.0
26	39.66385877452347	41.0	40.0	41.0	37.0	41.0
27	39.62701234424771	41.0	40.0	41.0	37.0	41.0
28	39.564803851010126	41.0	40.0	41.0	37.0	41.0
29	39.54797315363912	41.0	40.0	41.0	37.0	41.0
30	39.4872964754456	41.0	40.0	41.0	37.0	41.0
31	39.48651158556574	41.0	40.0	41.0	37.0	41.0
32	39.423742520848954	41.0	40.0	41.0	37.0	41.0
33	39.41125057814547	41.0	39.0	41.0	37.0	41.0
34	39.40689847459405	41.0	39.0	41.0	37.0	41.0
35	39.339608547041344	41.0	39.0	41.0	36.0	41.0
36	39.30847874567845	41.0	39.0	41.0	36.0	41.0
37	39.273871253892914	41.0	39.0	41.0	36.0	41.0
38	39.23486365156066	41.0	39.0	41.0	36.0	41.0
39	39.18271997184896	41.0	39.0	41.0	36.0	41.0
40	39.09683390600181	40.0	39.0	41.0	35.0	41.0
41	39.07619649067232	40.0	39.0	41.0	35.0	41.0
42	39.01976683949457	40.0	39.0	41.0	35.0	41.0
43	38.91794824796919	40.0	39.0	41.0	35.0	41.0
44	38.924618607690995	40.0	39.0	41.0	35.0	41.0
45	39.06495946552212	40.0	39.0	41.0	35.0	41.0
46	39.050212949397114	40.0	39.0	41.0	35.0	41.0
47	39.0786543998452	40.0	39.0	41.0	35.0	41.0
48	39.05944609071607	40.0	39.0	41.0	35.0	41.0
49	39.03626597834868	40.0	39.0	41.0	35.0	41.0
50	39.00652899218376	40.0	39.0	41.0	35.0	41.0
51	38.930788527144564	40.0	39.0	41.0	35.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	6.0
15	15.0
16	57.0
17	158.0
18	354.0
19	858.0
20	1672.0
21	3060.0
22	5497.0
23	9592.0
24	15932.0
25	24740.0
26	37367.0
27	54785.0
28	78236.0
29	107568.0
30	145420.0
31	190890.0
32	250615.0
33	328934.0
34	447503.0
35	623514.0
36	910596.0
37	1465545.0
38	2970961.0
39	1.6741387E7
40	81164.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.150757903339937	24.193673702531957	29.398403162107623	24.25716523202049
2	19.83928301124565	30.80233005669281	27.24828083735994	22.1101060947016
3	20.403627488268786	26.25533246743736	32.70249639549062	20.63854364880324
4	19.926699523155005	25.372737986718878	28.62442404872873	26.07613844139738
5	24.466256084371917	26.809630520037693	28.503588756487424	20.220524639102965
6	25.100228890495472	27.138178910151396	27.016516284209573	20.745075915143556
7	20.426878528830528	31.686592699425997	27.97697052182706	19.90955824991642
8	20.364232665739458	32.011546172238155	27.27874633160832	20.345474830414066
9	24.72026740222963	26.87214422673018	27.82039260414681	20.58719576689338
10	20.29891702586867	31.70558730394592	27.084840525741917	20.910655144443496
11	24.631732052384773	26.97767797185141	27.517670942255666	20.872919033508147
12	20.115420900106667	27.012866772831796	27.292270751289067	25.57944157577247
13	19.928601841166245	31.81270903556151	27.554962090394397	20.703727032877854
14	20.041291731080815	27.188790366596276	27.407532444523547	25.362385457799363
15	19.954613794744784	31.89251658302863	27.456735532483712	20.696134089742873
16	20.07592126689481	27.10101839329241	27.443754049059393	25.37930629075339
17	24.688944470684095	27.1157348234008	27.54567502285027	20.649645683064836
18	20.11128152142986	27.084738470054614	32.07257547324242	20.7314045352731
19	20.132488693250405	27.031110247493206	27.449526318732975	25.386874740523414
20	20.217733767736767	26.957450534628926	32.03663146017603	20.788184237458275
21	24.808843574349403	27.017132700560857	27.44884458674182	20.725179138347915
22	20.215072155412035	31.704974969822132	27.397775920817853	20.682176953947984
23	20.01168741730938	31.96409844209952	27.405213739308138	20.619000401282964
24	20.103157888720926	27.162696768467896	27.447583178446816	25.286562164364355
25	20.09075199937297	27.11292216865886	32.126522109548205	20.66980372241996
26	20.118854053427377	27.25892711713503	27.344012984749206	25.27820584468839
27	20.077488842251707	27.198787741723997	27.326492064353868	25.39723135167043
28	20.352096203405964	31.849967525880302	27.22157881781141	20.57635745290232
29	24.826413481474646	27.105063880736907	27.462675173484463	20.605847464303984
30	20.20318470895555	27.07621069682399	31.978953667942633	20.74165092627783
31	20.083097822825614	27.26589956169124	27.364567000171046	25.286435615312108
32	20.138636527853244	31.776777269177593	27.417946206855447	20.66663999611372
33	20.090621368093228	27.229800423980148	27.35764354234474	25.321934665581878
34	20.139816291598407	27.29385873778342	27.307987327132977	25.258337643485195
35	20.118511146318056	31.948149179288578	27.302635526891063	20.63070414750231
36	24.790877691157444	27.40470754309914	27.30648506741595	20.49792969832747
37	20.118898957929787	31.983448200411246	27.446031931999887	20.45162090965908
38	20.211598179816406	27.25048507068173	31.95861192835038	20.57930482115149
39	20.19637147127153	27.241447019014608	27.33409317194383	25.228088337770032
40	20.222905949969036	31.757084603756546	27.33722832265763	20.682781123616788
41	20.237650955669867	27.208450374197383	32.04105659477728	20.51284207535547
42	20.3638081140803	27.143044925321767	27.37132308667018	25.121823873927752
43	20.152001740661802	27.22054601425595	32.03896241207392	20.58848983300832
44	20.255829114691405	27.231037338910202	27.324704048712405	25.188429497685988
45	24.79991107204116	27.204893095195494	27.4801966490113	20.51499918375205
46	20.18163871225317	27.358794730497465	31.924753933532358	20.53481262371701
47	20.08686163657317	31.93646992643418	27.480032804780123	20.49663563221253
48	20.191590590202267	27.27348545782246	32.0224032228218	20.512520729153472
49	24.821445410616978	27.244533182998502	27.407014001632074	20.52700740475245
50	20.235791833992593	27.224979163224827	27.32865582072564	25.21057318205694
51	20.212169691665274	27.285714693937035	27.29719799987182	25.204917614525872
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.40792313536043
2	41.94938910594725
3	41.042171137072025
4	46.002837964552384
5	44.68678072347488
6	45.845304805639024
7	40.33643677874694
8	40.70970749615352
9	45.30746316912301
10	41.209572170312164
11	45.504651085892924
12	45.69486247587914
13	40.63232887404409
14	45.403677188880174
15	40.65074788448766
16	45.4552275576482
17	45.33859015374894
18	40.84268605670295
19	45.51936343377382
20	41.00591800519504
21	45.53402271269732
22	40.89724910936002
23	40.63068781859234
24	45.389720053085284
25	40.760555721792926
26	45.397059898115764
27	45.47472019392214
28	40.92845365630829
29	45.43226094577863
30	40.94483563523338
31	45.36953343813772
32	40.80527652396696
33	45.41255603367511
34	45.3981539350836
35	40.74921529382036
36	45.288807389484916
37	40.570519867588864
38	40.7909030009679
39	45.424459809041565
40	40.905687073585824
41	40.750493031025336
42	45.485631988008045
43	40.74049157367013
44	45.444258612377396
45	45.31491025579321
46	40.71645133597018
47	40.58349726878569
48	40.70411131935574
49	45.34845281536942
50	45.44636501604953
51	45.41708730619114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	287.0
1	409.0
2	531.0
3	721.5
4	912.0
5	1276.5
6	1641.0
7	2471.5
8	3302.0
9	4869.5
10	6437.0
11	9611.0
12	12785.0
13	18527.0
14	24269.0
15	34297.5
16	44326.0
17	60510.5
18	76695.0
19	100393.0
20	124091.0
21	158388.5
22	192686.0
23	238488.0
24	284290.0
25	344079.5
26	481118.5
27	558368.0
28	661762.5
29	765157.0
30	898946.0
31	1032735.0
32	1172874.0
33	1313013.0
34	1435907.5
35	1558802.0
36	1620342.5
37	1681883.0
38	1706032.0
39	1730181.0
40	1687032.5
41	1643884.0
42	1637792.0
43	1631700.0
44	1603517.0
45	1575334.0
46	1548236.5
47	1521139.0
48	1484245.5
49	1447352.0
50	1385260.0
51	1323168.0
52	1789518.0
53	2255868.0
54	1578800.5
55	901733.0
56	791782.5
57	681832.0
58	593153.5
59	504475.0
60	431936.0
61	359397.0
62	303651.0
63	247905.0
64	209124.0
65	170343.0
66	144427.5
67	118512.0
68	99789.0
69	81066.0
70	69589.5
71	58113.0
72	51118.0
73	44123.0
74	39129.0
75	29871.0
76	25607.0
77	22315.0
78	19023.0
79	16323.0
80	13623.0
81	11337.0
82	9051.0
83	7445.5
84	5840.0
85	4687.5
86	3535.0
87	2732.5
88	1930.0
89	1404.5
90	879.0
91	619.5
92	360.0
93	262.0
94	164.0
95	107.5
96	51.0
97	36.5
98	22.0
99	14.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01387549124505081
2	8.246099533687154E-4
3	1.2654905224965433E-4
4	0.0
5	0.033809008088117334
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	8.041988159090937E-4
46	0.0
47	0.0
48	5.429362564259363E-4
49	0.0
50	3.1433151687817367E-4
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.449643E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	33.02711836082924
#Duplication Level	Relative count
1	100.0
2	25.189046071099344
3	4.908613292485982
4	1.3508736666273904
5	0.7485600615482717
6	0.5406267111181963
7	0.443591147584161
8	0.37635936427843664
9	0.316751803821815
10++	4.747811501486724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC	1113539	4.54571951912993	TruSeq Adapter, Index 7 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	3982965	3.950926	57.10507	12
GGAAG	2818030	3.2506382	65.58499	5
TCCAG	3011405	3.1316164	58.14411	25
GTGTG	2656470	3.0454733	3.4918354	4
CCAGA	2778030	2.89783	57.954174	33
CTCCA	2825305	2.7939663	55.127922	24
CTGAA	3399435	2.7705107	46.223118	19
AGAGC	2451290	2.688897	61.83807	8
GCACA	2507485	2.615618	58.611843	11
GAGCA	2377110	2.6075268	61.660732	9
GTCTG	2376835	2.5912263	60.792023	17
CACCA	2594135	2.5732677	54.936073	31
GAAGA	2978975	2.5609436	49.00372	6
AAGAG	2879595	2.4755096	48.851204	7
ACCAG	2367810	2.4699194	57.510498	32
CCAGT	2356570	2.4506412	57.35064	26
AGCAC	2298430	2.3975477	58.496635	10
TCTGA	2876895	2.3374403	45.692547	18
CAGTC	2235285	2.3245146	57.228455	27
ATGCC	2109585	2.1937969	56.992252	47
CATCT	2794170	2.1588666	42.755646	39
TCACC	2156755	2.132832	54.355015	30
ACTCC	2134440	2.1107645	54.582985	23
GTCAC	2020265	2.1009114	57.014877	29
CAGAT	2546820	2.0756366	45.085327	34
CACGT	1905425	1.981487	57.647797	14
AGTCA	2424060	1.9755883	45.006996	28
GAACT	2349925	1.9151689	45.225132	21
AACTC	2459645	1.9062594	43.109074	22
ATCTC	2382640	1.8409052	42.473866	40
TGAAC	2222510	1.8113269	45.159256	20
TCATC	2314175	1.7880069	42.409046	38
GTATG	2059995	1.760055	46.751083	45
AGATC	2132345	1.737843	44.680656	35
ACGTC	1639520	1.7049675	57.315453	15
CGGAA	1529050	1.677263	61.035007	4
TCTCG	1567605	1.6251725	56.15797	41
GATCA	1964015	1.6006556	44.548126	36
TATGC	1967520	1.5985848	44.38593	46
ATCAT	2520185	1.5260162	33.477165	37
ACACG	1461220	1.5242337	57.400684	13
TCGGA	1345330	1.4712001	60.708294	3
CGTCT	1403560	1.4551032	56.845524	16
CTCGT	1385260	1.4361311	55.924572	42
ATCGG	1306985	1.4292675	60.69754	2
GATCG	1299875	1.4214922	60.62434	1
CGTAT	1335435	1.0850238	43.88064	44
TCGTA	1296120	1.0530809	43.826187	43
>>END_MODULE