FastQCFastQC Report
Tue 12 Aug 2014
SV39_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SV39_GCCAAT_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 26997017
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC 1048897 3.8852329499959195 TruSeq Adapter, Index 6 (100% over 51bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 4343685 3.6737866 46.53308 12
GGAAG 3127985 3.0635104 53.221306 5
GTGTG 3099865 3.0003328 3.4651349 6
TCCAG 3304670 2.9177816 47.14272 25
CTGAA 3550065 2.6783442 40.711517 19
CTCCA 3142935 2.6425734 44.74196 24
GAAGA 3169665 2.5260417 43.331524 6
AGAGC 2652495 2.4738665 50.134827 8
GCACA 2726415 2.4214785 47.58799 11
GAGCA 2590055 2.4156318 49.982944 9
AAGAG 3021200 2.4077234 43.116344 7
GTCTG 2531210 2.3330383 48.898716 17
TCTGA 2987980 2.24101 40.0829 18
CCAGT 2516415 2.2218099 46.302834 26
AGCAC 2457945 2.1830359 47.452335 10
CAGTC 2368805 2.0914812 46.1964 27
ATGCC 2201870 1.9440899 45.987152 47
ACTCC 2266145 1.9053699 44.099228 23
GCCAA 2119465 1.882413 46.039165 34
AGTCA 2481645 1.8722755 39.557903 28
GTCAC 2109360 1.8624102 45.961323 29
CGCCA 1770740 1.8405093 53.50976 33
GAACT 2409790 1.8180646 39.744232 21
AACTC 2495310 1.7927575 37.896965 22
ATCTC 2448140 1.7485148 37.295082 40
CACGT 1960610 1.7310749 46.48293 14
TGAAC 2260920 1.7057496 39.67899 20
TCACG 1881540 1.6612619 45.67998 30
GTATG 2037600 1.6047894 40.84993 45
AATAT 3066280 1.5990268 27.510208 37
CACGC 1480540 1.538875 53.24597 31
CCAAT 2105905 1.5129892 37.25778 35
TATCT 2436630 1.4783051 31.618982 39
TATGC 1956830 1.4676387 38.861073 46
ACGTC 1650285 1.4570806 46.18457 15
CGGAA 1539800 1.4361045 49.314487 4
ACGCC 1364965 1.4187461 53.109184 32
TCTCG 1578245 1.385272 45.068596 41
ACACG 1478160 1.3128347 46.36934 13
CAATA 2100130 1.2892842 31.847248 36
ATATC 2068870 1.2626172 31.584208 38
TCGGA 1340110 1.2425051 48.875843 3
CGTCT 1396900 1.2261003 45.607243 16
CTCGT 1377870 1.2093971 44.86997 42
ATCGG 1281910 1.1885438 48.79541 2
GATCG 1275750 1.1828326 48.728577 1
CGTAT 1283285 0.9624745 38.355118 44
TCGTA 1243910 0.9329429 38.308197 43
Produced by FastQC (version 0.10.1)