##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV39_GCCAAT_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	26997017	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91906098366349	34.0	31.0	34.0	31.0	34.0
2	33.07366099002716	34.0	33.0	34.0	31.0	34.0
3	33.119082674948864	34.0	33.0	34.0	31.0	34.0
4	36.45814194953465	37.0	37.0	37.0	35.0	37.0
5	36.3843544640506	37.0	37.0	37.0	35.0	37.0
6	36.395223146320205	37.0	37.0	37.0	35.0	37.0
7	36.37924464025044	37.0	37.0	37.0	35.0	37.0
8	36.394457802504625	37.0	37.0	37.0	35.0	37.0
9	38.21224648634329	39.0	39.0	39.0	37.0	39.0
10	38.194907533673074	39.0	39.0	39.0	37.0	39.0
11	38.19608077440556	39.0	39.0	39.0	37.0	39.0
12	38.191382477552985	39.0	39.0	39.0	37.0	39.0
13	38.18399958780631	39.0	39.0	39.0	37.0	39.0
14	39.76382431436777	41.0	40.0	41.0	38.0	41.0
15	39.74864497066472	41.0	40.0	41.0	37.0	41.0
16	39.748030865780464	41.0	40.0	41.0	37.0	41.0
17	39.72130091261564	41.0	40.0	41.0	37.0	41.0
18	39.68784999468645	41.0	40.0	41.0	37.0	41.0
19	39.74103831545537	41.0	40.0	41.0	37.0	41.0
20	39.71753086646573	41.0	40.0	41.0	37.0	41.0
21	39.712659513456614	41.0	40.0	41.0	37.0	41.0
22	39.68743061501943	41.0	40.0	41.0	37.0	41.0
23	39.67140006616287	41.0	40.0	41.0	37.0	41.0
24	39.659911982127504	41.0	40.0	41.0	37.0	41.0
25	39.624528813683376	41.0	40.0	41.0	37.0	41.0
26	39.580794241082266	41.0	40.0	41.0	37.0	41.0
27	39.54279782095926	41.0	40.0	41.0	37.0	41.0
28	39.48530198725289	41.0	40.0	41.0	37.0	41.0
29	39.452122210390876	41.0	40.0	41.0	37.0	41.0
30	39.411816794425846	41.0	39.0	41.0	37.0	41.0
31	39.38445473438788	41.0	39.0	41.0	36.0	41.0
32	39.31208329423951	41.0	39.0	41.0	36.0	41.0
33	39.28288147538671	41.0	39.0	41.0	36.0	41.0
34	39.23226803168661	41.0	39.0	41.0	36.0	41.0
35	39.22431007840607	41.0	39.0	41.0	36.0	41.0
36	39.19397191178566	41.0	39.0	41.0	36.0	41.0
37	39.122148865557996	41.0	39.0	41.0	36.0	41.0
38	39.09424089335499	41.0	39.0	41.0	35.0	41.0
39	39.01013382330351	40.0	39.0	41.0	35.0	41.0
40	38.937627738649795	40.0	39.0	41.0	35.0	41.0
41	38.898293911508816	40.0	39.0	41.0	35.0	41.0
42	38.82914771657921	40.0	38.0	41.0	35.0	41.0
43	38.73525760271959	40.0	38.0	41.0	35.0	41.0
44	38.724893272467845	40.0	38.0	41.0	35.0	41.0
45	38.87921306268763	40.0	39.0	41.0	35.0	41.0
46	38.875027859559445	40.0	39.0	41.0	35.0	41.0
47	38.88974637457168	40.0	39.0	41.0	35.0	41.0
48	38.86547858231893	40.0	39.0	41.0	35.0	41.0
49	38.83285408902769	40.0	39.0	41.0	35.0	41.0
50	38.799274897667395	40.0	39.0	41.0	35.0	41.0
51	38.717064222317596	40.0	38.0	41.0	35.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	10.0
15	24.0
16	81.0
17	251.0
18	543.0
19	1197.0
20	2510.0
21	4540.0
22	7959.0
23	13644.0
24	21562.0
25	34032.0
26	49780.0
27	70964.0
28	98742.0
29	133769.0
30	178507.0
31	232393.0
32	300086.0
33	392088.0
34	530766.0
35	739404.0
36	1081489.0
37	1736640.0
38	3436830.0
39	1.7850884E7
40	78320.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.6691759779025	23.138839207107292	29.574289893816143	25.61769492117406
2	20.808047343625553	29.273414060330182	26.185852015091843	23.732686580952418
3	21.434751746963208	25.199534274023357	31.191761333698263	22.173952645315175
4	20.92821958811227	24.208459771685146	27.797730393694977	27.065590246507604
5	24.866482775680577	25.79351759263002	27.911644436058854	21.428355195630548
6	25.603899127077632	26.256941646553024	26.073958467337338	22.06520075903201
7	21.698826948177274	30.0904948128158	27.155659456746644	21.055018782260277
8	21.55688163621929	30.48485690104207	26.344192026845043	21.6140694358936
9	25.108640706489904	25.948985400868548	27.05575953076594	21.886614361875612
10	21.404201804962376	30.086757362859757	26.201235492054547	22.30780534012332
11	25.085289978518738	25.93043149915415	26.661401146652608	22.322877375674505
12	21.31671065732929	26.034609675580082	26.323337870995157	26.325341796095476
13	21.10427607613093	30.22130926539032	26.606495080549088	22.067919577929665
14	21.25417782268315	26.293649405784354	26.394412390079985	26.057760381452518
15	21.12771940692559	30.270355424823414	26.58051072827787	22.021414439973128
16	21.282880993852025	26.236661628208775	26.49430861194776	25.98614876599144
17	25.22741679201076	26.19833887573579	26.630475507719982	21.943768824533468
18	21.361886018740513	26.1654426487193	30.519979299935247	21.952692032604936
19	21.286562882113977	26.141551120258953	26.56319029617235	26.00869570145472
20	21.44556563415877	26.078140410846128	30.411782160969857	22.064511794025243
21	25.353597399297854	26.118567099468805	26.534835311619798	21.99300018961354
22	21.368486747998862	30.14140414105751	26.477169681376278	22.01293942956735
23	21.254766776640547	30.299973511888368	26.509588077823558	21.935671633647523
24	21.352503500664536	26.228105127318322	26.50942880096716	25.909962571049977
25	21.29167085385767	26.243147529965995	30.54311148524298	21.922070130933356
26	21.38487744775654	26.391989900217496	26.355878503169443	25.86725414885652
27	21.35915608750404	26.338150618640572	26.411117939437535	25.89157535441786
28	21.53509404390863	30.346023043953334	26.295427379995353	21.82345553214268
29	25.3710511794692	26.23692091611455	26.524634184584173	21.867393719832084
30	21.50870223921406	26.195694139096926	30.352475608694103	21.94312801299492
31	21.32776743445396	26.300001959475743	26.463019969947048	25.90921063612324
32	21.401575588888207	30.22835819231436	26.428030919119692	21.94203529967774
33	21.39354507203518	26.290045303894132	26.404287555176932	25.912122068893762
34	25.37432191119485	26.335802211036874	26.411436493150337	21.878439384617938
35	21.33183084634869	26.423611912382768	26.39436794072471	25.850189300543835
36	21.375761625812213	26.490604499008167	26.38978965713138	25.74384421804824
37	21.333427319025656	30.49516174324	26.44368079628946	21.727730141444887
38	21.462771238763157	30.39020570309675	26.447933117944107	21.699089940195986
39	21.430801039981564	26.402772572984638	30.409274476509758	21.75715191052404
40	21.502264490925054	30.21989799836034	26.467664927573292	21.810172583141316
41	21.454677751990157	26.32022271201296	30.458831803528515	21.76626773246837
42	21.560330165366047	26.2355503943269	26.458652820791272	25.745466619515778
43	21.40878749678159	26.27452136656431	30.451082799258895	21.8656083373952
44	21.485851566489735	26.372535899058775	26.400535288769127	25.741077245682366
45	25.42308171851813	26.352732846722464	26.52439026648868	21.69979516827072
46	21.447980715795378	26.4845260496743	30.390735391247116	21.676757843283205
47	21.35000692854325	30.42589483126969	26.52334515328119	21.70075308690586
48	21.459476598583464	26.43949094839513	30.394605004209748	21.706427448811656
49	25.425668324763436	26.343569735871192	26.476802974195262	21.753958965170114
50	21.523449454489583	26.300335467922668	26.349622575436204	25.82659250215154
51	21.51588451420392	26.3295644848466	26.372613685430508	25.78193731551897
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.28687089907657
2	44.54073392457797
3	43.60870439227838
4	47.993809834619874
5	46.294837971311125
6	47.66909988610964
7	42.753845730437554
8	43.17095107211289
9	46.995255068365516
10	43.71200714508569
11	47.40816735419324
12	47.64205245342476
13	43.172195654060594
14	47.311938204135664
15	43.149133846898714
16	47.26902975984347
17	47.17118561654423
18	43.31457805134545
19	47.295258583568696
20	43.510077428184005
21	47.3465975889114
22	43.38142617756621
23	43.19043841028807
24	47.26246607171451
25	43.21374098479102
26	47.25213159661306
27	47.2507314419219
28	43.35854957605131
29	47.23844489930128
30	43.45183025220897
31	47.23697807057721
32	43.34361088856595
33	47.30566714092894
34	47.252761295812796
35	47.182020146892526
36	47.11960584386045
37	43.06115746047054
38	43.16186117895914
39	43.187952950505604
40	43.31243707406637
41	43.220945484458525
42	47.30579678488183
43	43.274395834176794
44	47.2269288121721
45	47.12287688678886
46	43.12473855907859
47	43.050760015449114
48	43.165904047395124
49	47.17962728993355
50	47.35004195664113
51	47.29782182972289
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	163.5
2	197.0
3	312.0
4	427.0
5	619.0
6	811.0
7	1194.5
8	1578.0
9	2378.0
10	3178.0
11	4910.0
12	6642.0
13	9973.0
14	13304.0
15	19176.5
16	25049.0
17	35571.0
18	46093.0
19	61865.5
20	77638.0
21	102154.5
22	126671.0
23	161811.0
24	196951.0
25	245509.0
26	362248.5
27	430430.0
28	529186.0
29	627942.0
30	763633.5
31	899325.0
32	1052191.0
33	1205057.0
34	1350441.5
35	1495826.0
36	1583450.0
37	1671074.0
38	1723435.5
39	1775797.0
40	1752621.0
41	1729445.0
42	1748980.0
43	1768515.0
44	1772939.0
45	1777363.0
46	1781473.0
47	1785583.0
48	1771374.0
49	1757165.0
50	1712597.0
51	1668029.0
52	2098113.0
53	2528197.0
54	1876088.0
55	1223979.0
56	1097301.0
57	970623.0
58	861540.5
59	752458.0
60	657123.0
61	561788.0
62	488074.0
63	414360.0
64	360387.0
65	306414.0
66	270726.5
67	235039.0
68	197439.5
69	159840.0
70	139389.5
71	118939.0
72	105268.0
73	91597.0
74	81715.5
75	63760.5
76	55687.0
77	48838.0
78	41989.0
79	36293.5
80	30598.0
81	25560.0
82	20522.0
83	16901.5
84	13281.0
85	10612.5
86	7944.0
87	6047.0
88	4150.0
89	3109.0
90	2068.0
91	1474.5
92	881.0
93	617.0
94	353.0
95	243.0
96	133.0
97	89.5
98	46.0
99	28.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013164417387298753
2	6.778526679447585E-4
3	9.630693642930994E-5
4	0.0
5	0.03306291209877003
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	8.260171855283123E-4
46	0.0
47	0.0
48	5.445046021195601E-4
49	0.0
50	3.667071810192956E-4
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.6997017E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.21193478210017
#Duplication Level	Relative count
1	100.0
2	8.386154811396539
3	1.3005517670030433
4	0.6836985393713022
5	0.5154125352550383
6	0.4245263657845822
7	0.3535765173592584
8	0.32777657247732245
9	0.29318119184018104
10++	4.98759843558516
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	1048897	3.8852329499959195	TruSeq Adapter, Index 6 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	4343685	3.6737866	46.53308	12
GGAAG	3127985	3.0635104	53.221306	5
GTGTG	3099865	3.0003328	3.4651349	6
TCCAG	3304670	2.9177816	47.14272	25
CTGAA	3550065	2.6783442	40.711517	19
CTCCA	3142935	2.6425734	44.74196	24
GAAGA	3169665	2.5260417	43.331524	6
AGAGC	2652495	2.4738665	50.134827	8
GCACA	2726415	2.4214785	47.58799	11
GAGCA	2590055	2.4156318	49.982944	9
AAGAG	3021200	2.4077234	43.116344	7
GTCTG	2531210	2.3330383	48.898716	17
TCTGA	2987980	2.24101	40.0829	18
CCAGT	2516415	2.2218099	46.302834	26
AGCAC	2457945	2.1830359	47.452335	10
CAGTC	2368805	2.0914812	46.1964	27
ATGCC	2201870	1.9440899	45.987152	47
ACTCC	2266145	1.9053699	44.099228	23
GCCAA	2119465	1.882413	46.039165	34
AGTCA	2481645	1.8722755	39.557903	28
GTCAC	2109360	1.8624102	45.961323	29
CGCCA	1770740	1.8405093	53.50976	33
GAACT	2409790	1.8180646	39.744232	21
AACTC	2495310	1.7927575	37.896965	22
ATCTC	2448140	1.7485148	37.295082	40
CACGT	1960610	1.7310749	46.48293	14
TGAAC	2260920	1.7057496	39.67899	20
TCACG	1881540	1.6612619	45.67998	30
GTATG	2037600	1.6047894	40.84993	45
AATAT	3066280	1.5990268	27.510208	37
CACGC	1480540	1.538875	53.24597	31
CCAAT	2105905	1.5129892	37.25778	35
TATCT	2436630	1.4783051	31.618982	39
TATGC	1956830	1.4676387	38.861073	46
ACGTC	1650285	1.4570806	46.18457	15
CGGAA	1539800	1.4361045	49.314487	4
ACGCC	1364965	1.4187461	53.109184	32
TCTCG	1578245	1.385272	45.068596	41
ACACG	1478160	1.3128347	46.36934	13
CAATA	2100130	1.2892842	31.847248	36
ATATC	2068870	1.2626172	31.584208	38
TCGGA	1340110	1.2425051	48.875843	3
CGTCT	1396900	1.2261003	45.607243	16
CTCGT	1377870	1.2093971	44.86997	42
ATCGG	1281910	1.1885438	48.79541	2
GATCG	1275750	1.1828326	48.728577	1
CGTAT	1283285	0.9624745	38.355118	44
TCGTA	1243910	0.9329429	38.308197	43
>>END_MODULE