FastQCFastQC Report
Tue 12 Aug 2014
SV38_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SV38_ACAGTG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 22649290
Filtered Sequences 0
Sequence length 51
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC 251246 1.1092886355378027 TruSeq Adapter, Index 5 (100% over 51bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 3293890 3.283319 15.105426 12
ACACA 3380945 3.0518303 13.800446 32
CACAG 2447270 2.5299625 14.799822 33
GGAAG 2129240 2.3676279 16.005913 5
CAGTG 2135400 2.2511246 14.627436 35
TCCAG 2137295 2.1724832 14.32009 25
CTCCA 2059705 2.0186858 13.717065 24
CTGAA 2140960 1.9706923 13.075667 19
GAAGA 1967045 1.909823 13.817488 6
AAGAG 1926095 1.8700644 13.752465 7
AGAGC 1708565 1.831859 14.966205 8
GCACA 1691855 1.7490225 14.345056 11
TCTGA 1930810 1.7474692 12.665328 18
GAGCA 1608835 1.7249322 14.828389 9
GTCTG 1600050 1.6584924 14.175734 17
TCACA 1849180 1.6411989 12.196201 30
AGCAC 1541455 1.5935405 14.249826 10
ACAGT 1698165 1.5631123 12.424166 34
AGTGA 1628735 1.5548517 12.875232 36
CCAGT 1514825 1.539765 13.619038 26
CAGTC 1423695 1.4471346 13.516089 27
AGTCA 1494670 1.375801 12.318395 28
GAACT 1440805 1.3262197 12.381555 21
AACTC 1468205 1.3030727 11.954479 22
ACTCC 1306660 1.2806377 13.022449 23
ATGCC 1209945 1.2298654 13.305239 47
ATCTC 1403405 1.2246859 11.560341 40
GTCAC 1188975 1.2085502 13.337182 29
TGAAC 1292180 1.1894146 12.274053 20
GTGAT 1266545 1.1888274 12.304329 37
GATCT 1159065 1.0490055 11.7416725 39
GTATG 1058370 0.9934266 12.122515 45
TGATC 1022135 0.9250778 11.602369 38
TATGC 976065 0.8833824 11.628157 46
CACGT 856175 0.870271 13.182642 14
CGGAA 659350 0.7069302 13.911282 4
TCTCG 655075 0.6547006 12.493748 41
ACACG 627410 0.64861 13.195022 13
ACGTC 622250 0.6324947 12.953337 15
CGTCT 557780 0.55746114 12.64512 16
TCGGA 508535 0.53609425 13.520286 3
CTCGT 522425 0.5221264 12.362227 42
ATCGG 435050 0.45862684 13.408113 2
GATCG 428360 0.45157427 13.352653 1
CGTAT 408875 0.37005013 11.114205 44
TCGTA 386065 0.34940606 11.08545 43
Produced by FastQC (version 0.10.1)