##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV38_ACAGTG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	22649290	
Filtered Sequences	0	
Sequence length	51	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95360159192628	34.0	31.0	34.0	31.0	34.0
2	33.123382940480695	34.0	33.0	34.0	31.0	34.0
3	33.1554855361912	34.0	33.0	34.0	31.0	34.0
4	36.47974519289567	37.0	37.0	37.0	35.0	37.0
5	36.401931848636316	37.0	37.0	37.0	35.0	37.0
6	36.41673677188115	37.0	37.0	37.0	35.0	37.0
7	36.404396385052245	37.0	37.0	37.0	35.0	37.0
8	36.41101076457584	37.0	37.0	37.0	35.0	37.0
9	38.24414372371054	39.0	39.0	39.0	37.0	39.0
10	38.23209628204681	39.0	39.0	39.0	37.0	39.0
11	38.2281445466944	39.0	39.0	39.0	37.0	39.0
12	38.21789437991213	39.0	39.0	39.0	37.0	39.0
13	38.20427205444409	39.0	39.0	39.0	37.0	39.0
14	39.786539533910336	41.0	40.0	41.0	38.0	41.0
15	39.76609876071171	41.0	40.0	41.0	38.0	41.0
16	39.75957325814628	41.0	40.0	41.0	38.0	41.0
17	39.74108384854448	41.0	40.0	41.0	37.0	41.0
18	39.73447216226204	41.0	40.0	41.0	37.0	41.0
19	39.763044095421975	41.0	40.0	41.0	38.0	41.0
20	39.73242706504266	41.0	40.0	41.0	37.0	41.0
21	39.72194086437147	41.0	40.0	41.0	37.0	41.0
22	39.68096841004729	41.0	40.0	41.0	37.0	41.0
23	39.65778971437957	41.0	40.0	41.0	37.0	41.0
24	39.648484786940344	41.0	40.0	41.0	37.0	41.0
25	39.6153584063783	41.0	40.0	41.0	37.0	41.0
26	39.56499064650592	41.0	40.0	41.0	37.0	41.0
27	39.519467850868615	41.0	40.0	41.0	37.0	41.0
28	39.46451950590946	41.0	40.0	41.0	37.0	41.0
29	39.42747370888889	41.0	40.0	41.0	36.0	41.0
30	39.39276511537448	41.0	39.0	41.0	36.0	41.0
31	39.35182122706716	41.0	39.0	41.0	36.0	41.0
32	39.29064743309834	41.0	39.0	41.0	36.0	41.0
33	39.23283303803342	41.0	39.0	41.0	36.0	41.0
34	39.19042133329566	41.0	39.0	41.0	36.0	41.0
35	39.15169835345832	41.0	39.0	41.0	36.0	41.0
36	39.098780977240345	41.0	39.0	41.0	35.0	41.0
37	39.04936817003977	41.0	39.0	41.0	35.0	41.0
38	38.97754896511105	40.0	39.0	41.0	35.0	41.0
39	38.897475461703216	40.0	39.0	41.0	35.0	41.0
40	38.81134022302686	40.0	38.0	41.0	35.0	41.0
41	38.764234596316264	40.0	38.0	41.0	35.0	41.0
42	38.70486726073974	40.0	38.0	41.0	35.0	41.0
43	38.652233425418636	40.0	38.0	41.0	35.0	41.0
44	38.58930995187929	40.0	38.0	41.0	35.0	41.0
45	38.74959665402315	40.0	38.0	41.0	35.0	41.0
46	38.773524026580965	40.0	38.0	41.0	35.0	41.0
47	38.756757452441114	40.0	38.0	41.0	35.0	41.0
48	38.71760788086514	40.0	38.0	41.0	35.0	41.0
49	38.67174851838623	40.0	38.0	41.0	35.0	41.0
50	38.62183878611648	40.0	38.0	41.0	35.0	41.0
51	38.547682465984586	40.0	38.0	41.0	35.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	15.0
15	49.0
16	142.0
17	339.0
18	777.0
19	1611.0
20	3062.0
21	5130.0
22	8791.0
23	13837.0
24	21443.0
25	31351.0
26	45354.0
27	63596.0
28	86139.0
29	115342.0
30	151502.0
31	196093.0
32	252005.0
33	328790.0
34	447837.0
35	631412.0
36	947608.0
37	1531021.0
38	2944893.0
39	1.4753328E7
40	67817.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.3662595946908	23.0701173596287	29.496185615565544	28.067437430114957
2	22.04891007882648	26.24178472315585	26.29582215464048	25.413483043377195
3	22.905712207942482	25.218719037347924	28.02198022955537	23.85358852515422
4	22.37231277448432	23.929156278187968	27.724039031687088	25.974491915640623
5	23.548314663116358	25.572336314066686	28.067883410784344	22.81146561203261
6	24.22895375528328	26.103392203464214	26.081431250162808	23.586222791089696
7	23.304982187079595	27.023822821819138	27.157734304254127	22.513460686847136
8	22.98663225204852	27.413221341596138	26.43665209814524	23.163494308210105
9	23.519324446814892	25.82892885384045	27.211003082215825	23.440743617128838
10	22.77085948389552	27.041880783017923	26.32836172789522	23.858898005191335
11	23.618974369616	25.781527809481002	26.692867635144413	23.90663018575858
12	22.785848916235345	25.95803223853816	26.325756789727183	24.930362055499312
13	22.598258046940987	27.230257548912128	26.56054560650687	23.610938797640014
14	22.76677547066597	26.159725095135432	26.376221947796157	24.697277486402445
15	22.618095313363025	27.241542670874008	26.574462157533414	23.565899858229553
16	22.760709055339042	26.092875317504433	26.477712987912643	24.66870263924388
17	23.805792587758823	26.091241712212614	26.592745291353502	23.51022040867506
18	22.835311835382036	26.095210931556796	27.595019534828687	23.474457698232484
19	22.702036134466024	26.092107081502334	26.553212043291424	24.652644740740218
20	22.86963520710804	26.04826464758939	27.559137615351297	23.52296252995127
21	23.88266033946318	26.045633218524735	26.55600683288527	23.51569960912682
22	22.771901459162738	27.20199617736362	26.500521649906023	23.525580713567624
23	22.70025682924277	27.275172864138348	26.56293861750192	23.461631689116967
24	22.830344792265013	26.14404689948338	26.470953394124052	24.554654914127553
25	22.71727281517434	26.177315050493853	27.655401118533955	23.45001101579785
26	22.84763010231226	26.286329505251597	26.370848710930893	24.49519168150525
27	22.852645712073095	26.2390167638809	26.43910692123241	24.4692306028136
28	22.945121017038502	27.393556266002157	26.336490018009396	23.324832698949947
29	23.986659184460088	26.167186697684564	26.463363752241243	23.38279036561411
30	22.936131772784048	26.19834882241342	27.476870135885058	23.38864926891748
31	22.827002524140934	26.19297117039872	26.450732009700967	24.52929429575938
32	22.898572096520464	27.347523917968292	26.37164785297906	23.382256132532188
33	22.834177141976635	26.239445033376324	26.400244775884808	24.526133048762237
34	22.888170004446057	27.395618140789402	26.392986270209796	23.323225584554745
35	22.82836239016764	26.329602384887117	26.42527867319461	24.41675655175063
36	22.92973863639876	27.476984929770428	26.37905647373494	23.214219960095882
37	24.034448761970022	26.326555931775346	26.430197149667826	23.20879815658681
38	22.992932670295623	26.3117651811602	27.494018576299744	23.201283572244428
39	24.096870144715353	26.300890668096	26.3861913552257	23.216047831962943
40	22.99920218249667	27.39481899874124	26.35502481534741	23.250954003414677
41	22.95439283085695	26.28876225259158	27.513308364191545	23.24353655235992
42	23.012906806350223	26.24497280047189	26.34698924337143	24.395131149806463
43	22.907768852798476	26.26322502824592	27.52994023212207	23.29906588683354
44	22.974614215280038	26.32696654067302	26.341991294208338	24.356427949838604
45	24.056186767644903	26.33252395624463	26.42248336082154	23.188805915288928
46	22.95943051636497	26.39425341809832	27.473510207163226	23.172805858373486
47	22.903203588280252	27.537304701383576	26.38181152698385	23.177680183352326
48	22.986142109276315	26.395994735352566	27.444738641867545	23.173124513503573
49	24.107236915594264	26.321615379554945	26.35975785554426	23.211389849306535
50	23.02615144090991	26.313084374170774	26.284385826802943	24.37637835811637
51	22.99846926769007	26.31415819215525	26.32349623321526	24.36387630693942
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.43369702480575
2	47.46239312220367
3	46.7593007330967
4	48.34680469012495
5	46.359780275148964
6	47.81517654637298
7	45.818442873926735
8	46.15012656025862
9	46.96006806394373
10	46.629757489086856
11	47.525604555374585
12	47.716210971734654
13	46.209196844581
14	47.46405295706841
15	46.18399517159257
16	47.42941169458292
17	47.316012996433884
18	46.30976953361452
19	47.35468087520624
20	46.39259773705931
21	47.39835994859
22	46.29748217273036
23	46.16188851835974
24	47.38499970639256
25	46.16728383097218
26	47.342821783817506
27	47.321876314886694
28	46.26995371598845
29	47.369449550074194
30	46.32478104170153
31	47.356296819900315
32	46.28082822905266
33	47.36031019073887
34	46.2113955890008
35	47.24511894191827
36	46.14395859649464
37	47.24324691855683
38	46.19421624254005
39	47.3129179766783
40	46.250156185911344
41	46.19792938321687
42	47.40803795615668
43	46.206834739632015
44	47.33104216511864
45	47.24499268293383
46	46.132236374738454
47	46.08088377163257
48	46.15926662277989
49	47.3186267649008
50	47.40252979902628
51	47.362345574629494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	154.5
2	194.0
3	268.5
4	343.0
5	477.0
6	611.0
7	943.5
8	1276.0
9	1907.0
10	2538.0
11	3982.0
12	5426.0
13	8057.5
14	10689.0
15	15508.5
16	20328.0
17	28406.5
18	36485.0
19	49439.0
20	62393.0
21	81980.0
22	101567.0
23	128786.5
24	156006.0
25	193292.0
26	281674.5
27	332771.0
28	401989.5
29	471208.0
30	559390.5
31	647573.0
32	748590.0
33	849607.0
34	950070.0
35	1050533.0
36	1127164.0
37	1203795.0
38	1266236.5
39	1328678.0
40	1357971.5
41	1387265.0
42	1428763.0
43	1470261.0
44	1502929.0
45	1535597.0
46	1557477.5
47	1579358.0
48	1577924.5
49	1576491.0
50	1541510.0
51	1506529.0
52	1556966.5
53	1607404.0
54	1379417.5
55	1151431.0
56	1046012.0
57	940593.0
58	845569.5
59	750546.0
60	664318.0
61	578090.0
62	508169.5
63	438249.0
64	387335.0
65	336421.0
66	300746.5
67	265072.0
68	235242.0
69	205412.0
70	188159.0
71	170906.0
72	159401.0
73	147896.0
74	137306.0
75	115024.0
76	103332.0
77	92975.5
78	82619.0
79	72954.0
80	63289.0
81	53882.0
82	44475.0
83	37726.0
84	30977.0
85	25092.0
86	19207.0
87	14760.0
88	10313.0
89	7600.0
90	4887.0
91	3452.5
92	2018.0
93	1398.0
94	778.0
95	552.0
96	326.0
97	207.5
98	89.0
99	59.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01400485401529143
2	7.726511515372006E-4
3	1.1920903480859666E-4
4	0.0
5	0.034800207865235515
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	8.168026459107547E-4
46	0.0
47	0.0
48	4.415149437355431E-4
49	0.0
50	2.649089662413259E-4
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.264929E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.184823382306972
#Duplication Level	Relative count
1	100.0
2	3.964999673707337
3	0.7874529594744513
4	0.451371516825825
5	0.30889038741815494
6	0.2572273824802593
7	0.23329925387744446
8	0.18870592329947142
9	0.16369015248743773
10++	2.4085836723151552
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC	251246	1.1092886355378027	TruSeq Adapter, Index 5 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	3293890	3.283319	15.105426	12
ACACA	3380945	3.0518303	13.800446	32
CACAG	2447270	2.5299625	14.799822	33
GGAAG	2129240	2.3676279	16.005913	5
CAGTG	2135400	2.2511246	14.627436	35
TCCAG	2137295	2.1724832	14.32009	25
CTCCA	2059705	2.0186858	13.717065	24
CTGAA	2140960	1.9706923	13.075667	19
GAAGA	1967045	1.909823	13.817488	6
AAGAG	1926095	1.8700644	13.752465	7
AGAGC	1708565	1.831859	14.966205	8
GCACA	1691855	1.7490225	14.345056	11
TCTGA	1930810	1.7474692	12.665328	18
GAGCA	1608835	1.7249322	14.828389	9
GTCTG	1600050	1.6584924	14.175734	17
TCACA	1849180	1.6411989	12.196201	30
AGCAC	1541455	1.5935405	14.249826	10
ACAGT	1698165	1.5631123	12.424166	34
AGTGA	1628735	1.5548517	12.875232	36
CCAGT	1514825	1.539765	13.619038	26
CAGTC	1423695	1.4471346	13.516089	27
AGTCA	1494670	1.375801	12.318395	28
GAACT	1440805	1.3262197	12.381555	21
AACTC	1468205	1.3030727	11.954479	22
ACTCC	1306660	1.2806377	13.022449	23
ATGCC	1209945	1.2298654	13.305239	47
ATCTC	1403405	1.2246859	11.560341	40
GTCAC	1188975	1.2085502	13.337182	29
TGAAC	1292180	1.1894146	12.274053	20
GTGAT	1266545	1.1888274	12.304329	37
GATCT	1159065	1.0490055	11.7416725	39
GTATG	1058370	0.9934266	12.122515	45
TGATC	1022135	0.9250778	11.602369	38
TATGC	976065	0.8833824	11.628157	46
CACGT	856175	0.870271	13.182642	14
CGGAA	659350	0.7069302	13.911282	4
TCTCG	655075	0.6547006	12.493748	41
ACACG	627410	0.64861	13.195022	13
ACGTC	622250	0.6324947	12.953337	15
CGTCT	557780	0.55746114	12.64512	16
TCGGA	508535	0.53609425	13.520286	3
CTCGT	522425	0.5221264	12.362227	42
ATCGG	435050	0.45862684	13.408113	2
GATCG	428360	0.45157427	13.352653	1
CGTAT	408875	0.37005013	11.114205	44
TCGTA	386065	0.34940606	11.08545	43
>>END_MODULE