##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV37_TGACCA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	18341618	
Filtered Sequences	0	
Sequence length	51	
%GC	54	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92384450488501	34.0	31.0	34.0	31.0	34.0
2	33.08712960874008	34.0	33.0	34.0	31.0	34.0
3	33.124671880092585	34.0	33.0	34.0	31.0	34.0
4	36.46753045451061	37.0	37.0	37.0	35.0	37.0
5	36.388980677713384	37.0	37.0	37.0	35.0	37.0
6	36.38971496407787	37.0	37.0	37.0	35.0	37.0
7	36.37103269733346	37.0	37.0	37.0	35.0	37.0
8	36.377276475826726	37.0	37.0	37.0	35.0	37.0
9	38.20011767773159	39.0	39.0	39.0	37.0	39.0
10	38.177563069953806	39.0	39.0	39.0	37.0	39.0
11	38.17512969684572	39.0	39.0	39.0	37.0	39.0
12	38.160578908578294	39.0	39.0	39.0	37.0	39.0
13	38.14061365796627	39.0	39.0	39.0	37.0	39.0
14	39.70399236316011	41.0	40.0	41.0	37.0	41.0
15	39.67191335028349	41.0	40.0	41.0	37.0	41.0
16	39.656866422580606	41.0	40.0	41.0	37.0	41.0
17	39.619008203093095	41.0	40.0	41.0	37.0	41.0
18	39.58426628446847	41.0	40.0	41.0	37.0	41.0
19	39.591354263293454	41.0	40.0	41.0	37.0	41.0
20	39.54241943104474	41.0	40.0	41.0	37.0	41.0
21	39.5079742692275	41.0	40.0	41.0	37.0	41.0
22	39.44136351547611	41.0	39.0	41.0	36.0	41.0
23	39.399284566934064	41.0	39.0	41.0	36.0	41.0
24	39.35241994463084	41.0	39.0	41.0	36.0	41.0
25	39.28210668219129	41.0	39.0	41.0	36.0	41.0
26	39.19457165665537	41.0	39.0	41.0	35.0	41.0
27	39.10945321181588	41.0	39.0	41.0	35.0	41.0
28	39.012101222476666	41.0	39.0	41.0	35.0	41.0
29	38.922752343877185	41.0	39.0	41.0	35.0	41.0
30	38.820805449115774	40.0	38.0	41.0	35.0	41.0
31	38.73492616627388	40.0	38.0	41.0	35.0	41.0
32	38.621356796330616	40.0	38.0	41.0	35.0	41.0
33	38.509922570625996	40.0	38.0	41.0	34.0	41.0
34	38.41541155202338	40.0	38.0	41.0	34.0	41.0
35	38.303859833957944	40.0	38.0	41.0	34.0	41.0
36	38.17930555526781	40.0	37.0	41.0	33.0	41.0
37	38.06925217829747	40.0	37.0	41.0	33.0	41.0
38	37.94046037814112	40.0	37.0	41.0	33.0	41.0
39	37.76225668858658	40.0	36.0	41.0	33.0	41.0
40	37.600488081258696	40.0	36.0	41.0	33.0	41.0
41	37.465650358654294	40.0	36.0	41.0	33.0	41.0
42	37.317196171024825	40.0	35.0	41.0	32.0	41.0
43	37.17344304084841	40.0	35.0	41.0	32.0	41.0
44	37.048453249871415	39.0	35.0	41.0	31.0	41.0
45	37.235647367642265	39.0	35.0	41.0	33.0	41.0
46	37.21404000454049	39.0	35.0	41.0	33.0	41.0
47	37.151356984972644	39.0	35.0	41.0	33.0	41.0
48	37.05766361506384	39.0	35.0	41.0	33.0	41.0
49	36.95434045131678	39.0	35.0	41.0	32.0	41.0
50	36.845949795705046	39.0	35.0	41.0	32.0	41.0
51	36.71204808648834	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	12.0
14	37.0
15	136.0
16	360.0
17	904.0
18	2117.0
19	4142.0
20	7569.0
21	12252.0
22	19019.0
23	27149.0
24	38044.0
25	51322.0
26	66974.0
27	86635.0
28	110730.0
29	139905.0
30	175210.0
31	220020.0
32	278629.0
33	357629.0
34	497670.0
35	741131.0
36	1228356.0
37	2045732.0
38	3108050.0
39	9090858.0
40	31022.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.022296958375954	20.288553748550303	24.651763385533588	31.037385907540155
2	25.170071958669034	23.00414780665988	22.45565398382608	29.370126250845008
3	26.28366691753542	20.815614955208915	25.046413627254683	27.854304500000982
4	25.858013180734655	20.032093133768242	23.40365501015232	30.706238675344782
5	28.41507306107585	21.77540260478452	23.509625880802357	26.299898453337267
6	28.559312488134907	22.13853761429335	21.905651944119654	27.396497953452087
7	27.04663787022497	23.979214919861487	22.7275423574954	26.246604852418145
8	26.417118707847916	24.246465061043143	22.321525832671902	27.014890398437043
9	27.971147365515954	21.90045611025156	22.842728487748467	27.285668036484022
10	26.32538198102261	23.87648679631208	22.18606341054535	27.612067812119957
11	28.02737468417454	21.918622446503903	22.412313897279944	27.641688972041617
12	26.344856816884963	21.941237681430287	22.215864489163387	29.49804101252136
13	26.23507369960491	23.988107265127866	22.351343267535068	27.42547576773216
14	26.379210383729507	22.092385742631865	22.23430342950115	29.294100444137477
15	26.321609140480405	24.02642994745611	22.27782739777919	27.37413351428429
16	26.368578824398153	22.05434111647075	22.28157297791285	29.29550708121824
17	28.263318972186642	22.06984138476769	22.33748952791406	27.32935011513161
18	26.46239824643606	22.06674460235733	24.1423466566581	27.328510494548517
19	26.453620394885558	22.033208847769046	22.307568503498437	29.205602253846962
20	26.537402534498323	21.99860448516592	24.116574666422558	27.347418313913202
21	28.402199849544353	22.022233807290064	22.279980970054005	27.295585373111575
22	26.50980409689047	23.912247000237382	22.262185375357834	27.315763527514314
23	26.42968575618574	24.006333574278997	22.29408005335189	27.269900616183367
24	26.522840024255224	22.05403579989508	22.287521199056705	29.135602976792995
25	26.48015022447856	22.069187134962682	24.191164596274987	27.25949804428377
26	26.58188606915704	22.154392267901336	22.185016610857343	29.07870505208428
27	26.627067470274433	22.13732725215409	22.163933410891012	29.071671866680465
28	26.742226340118957	24.01885700596316	22.126123224243358	27.112793429674525
29	28.552600975551883	22.101539787820247	22.22785361684013	27.118005619787745
30	26.725401215966883	22.08605587576843	24.016180033844343	27.172362874420347
31	26.613966117929184	22.15723280247141	22.185163817063465	29.043637262535942
32	26.65361910819427	23.99256706796532	22.1952610723874	27.15855275145301
33	26.622553146619886	22.122851975218328	22.208406041386315	29.046188836775467
34	26.69150017190414	22.142577606839268	24.0454740688635	27.120448152393102
35	28.540339243789724	22.205887179637042	22.15252220387536	27.10125137269787
36	26.719273076126655	24.09289082348133	22.118572091077244	27.069264009314775
37	26.69385547120216	22.240529706812126	22.13480293832311	28.93081188366261
38	26.815791278610206	22.20338467413289	22.05176773390439	28.929056313352508
39	26.785488608475	24.081381478994928	22.0716024071595	27.061527505370574
40	26.773412247490924	24.017695712559274	22.120616621717886	27.088275418231916
41	26.790117425845416	22.11716000191477	24.028267298991835	27.06445527324798
42	26.803785794688338	22.06156512473436	22.137016483496712	28.997632597080585
43	26.721868267019843	22.09200954899399	24.037623071203424	27.148499112782744
44	26.795166053507387	22.115955091857217	22.085363461391466	29.003515393243934
45	28.622973738259734	22.1343400281973	22.185851703404627	27.056834530138335
46	26.760616211721345	22.229800010010024	23.952357965365977	27.057225812902658
47	26.765921087223603	24.077510500981976	22.156458606868817	27.00010980492561
48	26.864277204325138	22.182519977066256	23.96491743437789	26.98828538423072
49	28.687398243710017	22.162755761242	22.11044303725004	27.039402957797943
50	26.82979275806341	22.154551974265217	22.053486405026227	28.96216886264515
51	26.840467400422362	22.14239768814289	22.072360246516965	28.944774664917784
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	55.059682865916116
2	54.540198209514045
3	54.1379714175364
4	56.56425185607944
5	54.71497151441312
6	55.955810441587
7	53.29324272264312
8	53.43200910628495
9	55.25681540199998
10	53.93744979314257
11	55.66906365621615
12	55.842897829406326
13	53.66054946733707
14	55.673310827866985
15	53.6957426547647
16	55.6640859056164
17	55.59266908731825
18	53.79090874098458
19	55.65922264873252
20	53.88482084841152
21	55.697785222655924
22	53.82556762440478
23	53.69958637236911
24	55.65844300104822
25	53.73964826876233
26	55.66059112124132
27	55.698739336954894
28	53.85501976979348
29	55.67060659533962
30	53.89776409038723
31	55.657603380465126
32	53.81217185964727
33	55.66874198339535
34	53.811948324297234
35	55.641590616487605
36	53.788537085441426
37	55.62466735486476
38	55.744847591962724
39	53.84701611384557
40	53.86168766572283
41	53.854572699093396
42	55.801418391768934
43	53.87036737980259
44	55.79868144675132
45	55.67980826839807
46	53.817842024624
47	53.76603089214921
48	53.852562588555855
49	55.72680120150796
50	55.791961620708555
51	55.78524206534014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	108.0
1	140.5
2	173.0
3	260.0
4	347.0
5	450.0
6	553.0
7	800.5
8	1048.0
9	1622.5
10	2197.0
11	3077.5
12	3958.0
13	5594.0
14	7230.0
15	10226.5
16	13223.0
17	17986.0
18	22749.0
19	30207.5
20	37666.0
21	48109.0
22	58552.0
23	72802.5
24	87053.0
25	106028.5
26	150675.5
27	176347.0
28	210418.5
29	244490.0
30	288331.0
31	332172.0
32	380876.0
33	429580.0
34	476265.0
35	522950.0
36	555877.5
37	588805.0
38	613992.5
39	639180.0
40	647947.5
41	656715.0
42	680479.0
43	704243.0
44	723551.5
45	742860.0
46	762977.5
47	783095.0
48	802945.0
49	822795.0
50	834443.5
51	846092.0
52	1014601.5
53	1183111.0
54	1000454.0
55	817797.0
56	803312.0
57	788827.0
58	781171.0
59	773515.0
60	766228.5
61	758942.0
62	748779.5
63	738617.0
64	732162.5
65	725708.0
66	716775.5
67	707843.0
68	689672.5
69	671502.0
70	652511.5
71	633521.0
72	611197.0
73	588873.0
74	560144.0
75	490866.5
76	450318.0
77	409434.0
78	368550.0
79	325049.0
80	281548.0
81	240057.0
82	198566.0
83	164082.0
84	129598.0
85	102798.5
86	75999.0
87	57327.0
88	38655.0
89	28381.0
90	18107.0
91	12717.5
92	7328.0
93	5030.0
94	2732.0
95	1855.5
96	979.0
97	641.5
98	304.0
99	191.0
100	78.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013275818959919456
2	6.378935598811402E-4
3	1.1994579758448792E-4
4	0.0
5	0.03383016700053398
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	7.905518477159431E-4
46	0.0
47	0.0
48	4.6887902692118E-4
49	0.0
50	3.2712490250314885E-4
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.8341618E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.236631986450522
#Duplication Level	Relative count
1	100.0
2	18.955337461950247
3	5.892463524719219
4	1.935289178125328
5	0.7780518526293692
6	0.3968982890731605
7	0.2374829432140233
8	0.19156082712291383
9	0.15547916447989923
10++	2.66348273328435
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	338475	1.8453933562458884	TruSeq Adapter, Index 4 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2102390	4.2326603	4.4126506	44
TTTTT	2064800	3.9745064	4.1769733	1
GGAAG	1962670	2.3184721	21.92639	5
CACAC	2016235	2.1207051	19.331879	12
GAAGA	1476840	2.0838666	25.4321	6
CTGAA	1528475	2.0563195	23.8482	19
AAGAG	1439795	2.031595	25.326326	7
TCCAG	1845730	1.9999238	19.436052	25
CTCCA	1841725	1.9198395	18.663794	24
TCTGA	1364050	1.8187106	23.416986	18
AGAGC	1563295	1.7766031	20.519934	8
ACTGA	1294005	1.7408777	23.340794	32
CACTG	1551495	1.6811082	19.038588	31
GAGCA	1426720	1.6213926	20.340204	9
TCACT	1237325	1.5871278	22.162539	30
GAACT	1137010	1.5296661	23.222874	21
AATCT	943875	1.5032446	26.789421	39
GTCTG	1346700	1.5032191	19.554626	17
GCACA	1359455	1.4863089	19.413757	11
AACTC	1135475	1.4696189	22.320738	22
AGTCA	1079410	1.4521743	23.030025	28
AGCAC	1327825	1.4517277	19.45307	10
ACCAA	1069390	1.3965684	22.16236	36
ATCTC	1080665	1.3861786	21.762548	40
TGAAC	994295	1.3376656	23.056581	20
CCAGT	1229055	1.3317313	18.737879	26
CAGTC	1226795	1.3292826	18.726677	27
CTGAC	1219510	1.3213888	18.681097	33
ACTCC	1260325	1.3137802	18.102596	23
GACCA	1086525	1.1879112	18.62913	35
GTCAC	1077320	1.1673203	18.548185	29
TGACC	1064840	1.1537976	18.456522	34
CCAAT	872755	1.1295866	21.697245	37
CGGAA	991445	1.1267254	20.023441	4
CAATC	820920	1.0624977	21.595661	38
GTATG	755630	1.0472435	23.121254	45
ATGCC	957365	1.0373441	18.322216	47
TCTCG	930715	0.9994545	18.050262	41
TATGC	725645	0.9675147	22.214014	46
CACGT	885320	0.9592804	18.642141	14
TCGGA	795385	0.8958347	19.647532	3
CGTCT	787670	0.8458447	18.271881	16
ACACG	767545	0.8391666	18.695675	13
ACGTC	738220	0.7998916	18.406616	15
CTCGT	731640	0.7856765	17.841316	42
GATCG	634065	0.7141415	19.333542	1
ATCGG	607340	0.6840413	19.367832	2
TCGTA	492055	0.6560651	21.876953	43
CGTAT	474805	0.6330654	21.851694	44
>>END_MODULE