##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV36_CGATGT_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29611258	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96129863851107	34.0	31.0	34.0	31.0	34.0
2	33.13895829755021	34.0	33.0	34.0	31.0	34.0
3	33.16683519491134	34.0	33.0	34.0	31.0	34.0
4	36.48630385780976	37.0	37.0	37.0	35.0	37.0
5	36.4057526026081	37.0	37.0	37.0	35.0	37.0
6	36.42413263901182	37.0	37.0	37.0	35.0	37.0
7	36.41845321127525	37.0	37.0	37.0	35.0	37.0
8	36.42038629361846	37.0	37.0	37.0	35.0	37.0
9	38.253058583326656	39.0	39.0	39.0	37.0	39.0
10	38.24658493063686	39.0	39.0	39.0	37.0	39.0
11	38.23803862031124	39.0	39.0	39.0	37.0	39.0
12	38.22796961209821	39.0	39.0	39.0	37.0	39.0
13	38.21474160267017	39.0	39.0	39.0	37.0	39.0
14	39.797612752555125	41.0	40.0	41.0	38.0	41.0
15	39.77695652106371	41.0	40.0	41.0	38.0	41.0
16	39.76881073407959	41.0	40.0	41.0	38.0	41.0
17	39.75657103119361	41.0	40.0	41.0	38.0	41.0
18	39.76205131170043	41.0	40.0	41.0	38.0	41.0
19	39.78849594974992	41.0	40.0	41.0	38.0	41.0
20	39.757096810949406	41.0	40.0	41.0	38.0	41.0
21	39.748868893040616	41.0	40.0	41.0	38.0	41.0
22	39.72347436235232	41.0	40.0	41.0	37.0	41.0
23	39.698712563984955	41.0	40.0	41.0	37.0	41.0
24	39.692202911473736	41.0	40.0	41.0	37.0	41.0
25	39.66261973739853	41.0	40.0	41.0	37.0	41.0
26	39.61420379370576	41.0	40.0	41.0	37.0	41.0
27	39.57512571738762	41.0	40.0	41.0	37.0	41.0
28	39.53805191255299	41.0	40.0	41.0	37.0	41.0
29	39.503267000679266	41.0	40.0	41.0	37.0	41.0
30	39.48264882903658	41.0	40.0	41.0	37.0	41.0
31	39.44504630637442	41.0	40.0	41.0	37.0	41.0
32	39.39717036000294	41.0	39.0	41.0	36.0	41.0
33	39.344916213961596	41.0	39.0	41.0	36.0	41.0
34	39.322193302290636	41.0	39.0	41.0	36.0	41.0
35	39.28385447183636	41.0	39.0	41.0	36.0	41.0
36	39.24230169484863	41.0	39.0	41.0	36.0	41.0
37	39.20323776855411	41.0	39.0	41.0	36.0	41.0
38	39.163010500938526	41.0	39.0	41.0	36.0	41.0
39	39.08301001598784	40.0	39.0	41.0	35.0	41.0
40	39.017437827193966	40.0	39.0	41.0	35.0	41.0
41	38.968371320124255	40.0	39.0	41.0	35.0	41.0
42	38.91938964565436	40.0	39.0	41.0	35.0	41.0
43	38.8778449736921	40.0	38.0	41.0	35.0	41.0
44	38.823255060625925	40.0	38.0	41.0	35.0	41.0
45	38.980796695635156	40.0	39.0	41.0	35.0	41.0
46	39.01908321490428	40.0	39.0	41.0	35.0	41.0
47	39.000422609535875	40.0	39.0	41.0	35.0	41.0
48	38.96455719645548	40.0	39.0	41.0	35.0	41.0
49	38.92419785069584	40.0	39.0	41.0	35.0	41.0
50	38.880608787374044	40.0	39.0	41.0	35.0	41.0
51	38.82149059658323	40.0	39.0	41.0	35.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	5.0
15	38.0
16	110.0
17	339.0
18	654.0
19	1295.0
20	2436.0
21	4659.0
22	8018.0
23	13625.0
24	21429.0
25	33161.0
26	49374.0
27	70449.0
28	98364.0
29	135366.0
30	179881.0
31	237465.0
32	309123.0
33	405024.0
34	555529.0
35	774256.0
36	1129128.0
37	1820857.0
38	3749161.0
39	1.9924569E7
40	86939.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.779839964192554	23.317005912977596	31.64854998855179	28.25460413427806
2	21.69718281153652	26.112822015378246	26.877418417525057	25.31257675556018
3	23.144641157377276	26.217060381086547	27.381095264343326	23.25720319719285
4	22.32675153483854	24.439245370797824	28.42058922319342	24.813413871170216
5	22.044188745341728	26.25589876488424	29.02603794073682	22.673874549037212
6	22.98530849314136	26.92366193965822	26.657756316871108	23.433273250329318
7	23.134805687755648	26.614431578692134	27.996274254879683	22.25448847867254
8	22.882232831850644	27.260868146837936	27.011084770528832	22.84581425078259
9	22.17256693383307	26.625258541869446	27.943176206833225	23.258998317464254
10	22.501526277606985	26.827870669999903	26.978681554157546	23.69192149823557
11	22.235870559771556	26.565210434490826	27.474212679515336	23.72470632622228
12	22.488362365421963	26.82632058388063	27.07557375644088	23.60974329425653
13	22.249574806987262	27.037817846171887	27.389781953877133	23.32282539296372
14	22.45099482095627	27.031100806321707	27.177038543921366	23.340865828800656
15	22.30014003457739	27.003746345393363	27.41519458578896	23.280919034240288
16	22.39939620261996	26.94348210400247	27.346727383213505	23.31039431016406
17	22.395887402014463	26.9103528124337	27.458701687040787	23.235058098511047
18	22.473067506959683	26.932601107322085	27.42011163456818	23.17421975115005
19	22.33030086057134	26.924914841510617	27.40764340373516	23.33714089418288
20	22.48700477365737	26.899026039353007	27.386094167292725	23.2278750196969
21	22.46678611222799	26.892656840178823	27.42742979713999	23.213127250453187
22	22.425791568868842	27.004442026745366	27.34024673993925	23.229519664446542
23	22.38565143027696	27.081598492033	27.364332173931956	23.16841790375809
24	22.474138045739224	26.996607168800463	27.30207544711542	23.227179338344893
25	22.367985851867555	27.042285741456844	27.457475801939925	23.132252604735672
26	22.514200511170447	27.14611787179052	27.200354000495352	23.13932761654368
27	22.493515810777104	27.093222449380573	27.282711190453306	23.13055054938902
28	22.5685514610693	27.243469358849936	27.184093293165727	23.003885886915036
29	22.578068111797208	27.03889851623325	27.300103224253427	23.082930147716116
30	22.597401974613845	27.049948367610728	27.307546339301087	23.045103318474343
31	22.467100181964575	27.059826367390404	27.29671599902983	23.176357451615193
32	22.546566579508376	27.19597728674682	27.233263780957905	23.024192352786905
33	22.487031790408903	27.061052252491262	27.262617481499774	23.189298475600058
34	22.52469989623541	27.145108120701934	27.254424651597038	23.07576733146562
35	22.568163095265998	27.190627969943055	27.292980933130227	22.94822800166072
36	22.55985544416924	27.307603749898096	27.239936918586842	22.89260388734582
37	22.50247861809856	27.188132297520085	27.400115861338953	22.909273223042398
38	22.726200960459025	27.184660644947943	27.206892054366623	22.88224634022641
39	22.559240813071842	27.145027070447327	27.36280235037633	22.9329297661045
40	22.65452889573283	27.234013495812974	27.208904802355917	22.902552806098274
41	22.584339375247076	27.164036732245556	27.354829031579815	22.896794860927557
42	22.640243788359143	27.12246132872842	27.188480138196088	23.04881474471635
43	22.52932989202958	27.152071688409862	27.372028571025247	22.94656984853531
44	22.597540435465458	27.195899613586157	27.199388151628007	23.007171799320382
45	22.673505250041153	27.172997769815723	27.26737821741011	22.88611876273302
46	22.574089895133802	27.26323548969112	27.308549336201793	22.85412527897329
47	22.52829987837734	27.35115813046511	27.257058784871617	22.863483206285935
48	22.614355078762575	27.242443462297107	27.303342961373144	22.839858497567178
49	22.712192774788562	27.183647516765415	27.244921509244897	22.85923819920113
50	22.64470143538107	27.168701693256807	27.176306933726778	23.01028993763534
51	22.613075067597602	27.195372786931244	27.193508631075385	22.998043514395775
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.034444098470615
2	47.0097595670967
3	46.40184435457012
4	47.140165406008755
5	44.71806329437894
6	46.418581743470675
7	45.38929416642819
8	45.72804708263323
9	45.43156525129733
10	46.19344777584256
11	45.96057688599383
12	46.09810565967849
13	45.57240019995098
14	45.79186064975693
15	45.58105906881767
16	45.70979051278402
17	45.63094550052551
18	45.647287258109735
19	45.66744175475422
20	45.71487979335427
21	45.67991336268118
22	45.65531123331538
23	45.55406933403505
24	45.70131738408412
25	45.50023845660323
26	45.653528127714125
27	45.62406636016612
28	45.57243734798434
29	45.66099825951333
30	45.642505293088185
31	45.64345763357977
32	45.57075893229528
33	45.67633026600896
34	45.60046722770103
35	45.51639109692672
36	45.45245933151506
37	45.41175184114096
38	45.608447300685434
39	45.49217057917634
40	45.55708170183111
41	45.48113423617463
42	45.689058533075496
43	45.47589974056489
44	45.604712234785836
45	45.55962401277417
46	45.42821517410709
47	45.391783084663274
48	45.45421357632976
49	45.57143097398969
50	45.654991373016415
51	45.611118581993374
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	194.0
2	256.0
3	399.0
4	542.0
5	743.5
6	945.0
7	1443.0
8	1941.0
9	2863.0
10	3785.0
11	5843.0
12	7901.0
13	11754.0
14	15607.0
15	22428.0
16	29249.0
17	40656.5
18	52064.0
19	70782.5
20	89501.0
21	116698.5
22	143896.0
23	182389.5
24	220883.0
25	272834.0
26	393735.0
27	462685.0
28	555967.5
29	649250.0
30	768840.5
31	888431.0
32	1023596.0
33	1158761.0
34	1296292.5
35	1433824.0
36	1539752.0
37	1645680.0
38	1734007.0
39	1822334.0
40	1864055.5
41	1905777.0
42	1967054.0
43	2028331.0
44	2078869.0
45	2129407.0
46	2159784.0
47	2190161.0
48	2188909.0
49	2187657.0
50	2136253.5
51	2084850.0
52	1996600.5
53	1908351.0
54	1750650.0
55	1592949.0
56	1444256.5
57	1295564.0
58	1150055.5
59	1004547.0
60	874027.0
61	743507.0
62	634333.5
63	525160.0
64	442770.5
65	360381.0
66	300942.0
67	241503.0
68	196731.5
69	151960.0
70	125920.0
71	99880.0
72	83848.0
73	67816.0
74	57646.5
75	39831.0
76	32185.0
77	27352.0
78	22519.0
79	18853.5
80	15188.0
81	12512.0
82	9836.0
83	7996.5
84	6157.0
85	4911.5
86	3666.0
87	2837.5
88	2009.0
89	1532.0
90	1055.0
91	768.0
92	481.0
93	366.5
94	252.0
95	170.5
96	89.0
97	79.0
98	69.0
99	45.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013538769612557493
2	7.125668217135523E-4
3	1.0806700613665249E-4
4	0.0
5	0.03426399513320238
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	8.510276733261384E-4
46	0.0
47	0.0
48	5.977456276933591E-4
49	0.0
50	3.275781123517279E-4
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9611258E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	7.681109356494271
#Duplication Level	Relative count
1	100.0
2	2.993986369103301
3	0.5575304022450889
4	0.37631965789122007
5	0.2683415742349325
6	0.2218361619671255
7	0.19617800347454228
8	0.158225310704263
9	0.15074168114392622
10++	1.9858345583322197
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	33630	0.11357166926173823	TruSeq Adapter, Index 2 (100% over 51bp)
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE