##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV35_CAGATC_L001_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29368635	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74452411560837	34.0	31.0	34.0	31.0	34.0
2	32.94519125590958	34.0	31.0	34.0	31.0	34.0
3	32.98840092500043	34.0	31.0	34.0	31.0	34.0
4	36.37350741701138	37.0	37.0	37.0	35.0	37.0
5	36.28799714389177	37.0	37.0	37.0	35.0	37.0
6	36.278945684741565	37.0	37.0	37.0	35.0	37.0
7	36.27799501066359	37.0	37.0	37.0	35.0	37.0
8	36.27762900795355	37.0	37.0	37.0	35.0	37.0
9	38.06995095277666	39.0	39.0	39.0	35.0	39.0
10	38.07097851159919	39.0	39.0	39.0	35.0	39.0
11	38.060652938074924	39.0	38.0	39.0	35.0	39.0
12	38.05539508390499	39.0	39.0	39.0	35.0	39.0
13	38.02788239902876	39.0	38.0	39.0	35.0	39.0
14	39.552861854151544	41.0	40.0	41.0	37.0	41.0
15	39.523098298576016	41.0	39.0	41.0	37.0	41.0
16	39.52283573955685	41.0	39.0	41.0	37.0	41.0
17	39.51491249082567	41.0	39.0	41.0	37.0	41.0
18	39.54286190011895	41.0	40.0	41.0	37.0	41.0
19	39.56506827777321	41.0	40.0	41.0	37.0	41.0
20	39.53622059724601	41.0	40.0	41.0	37.0	41.0
21	39.5265033257419	41.0	40.0	41.0	37.0	41.0
22	39.498694678864034	41.0	39.0	41.0	37.0	41.0
23	39.463800241311866	41.0	39.0	41.0	37.0	41.0
24	39.45913294914796	41.0	39.0	41.0	36.0	41.0
25	39.43178356774157	41.0	39.0	41.0	36.0	41.0
26	39.37340060237733	41.0	39.0	41.0	36.0	41.0
27	39.33400455962628	41.0	39.0	41.0	36.0	41.0
28	39.286745877021524	41.0	39.0	41.0	36.0	41.0
29	39.272070118342235	41.0	39.0	41.0	36.0	41.0
30	39.26735818671859	41.0	39.0	41.0	36.0	41.0
31	39.21417546304076	41.0	39.0	41.0	36.0	41.0
32	39.156038678678804	41.0	39.0	41.0	36.0	41.0
33	39.12558823384199	41.0	39.0	41.0	36.0	41.0
34	39.08737307675348	40.0	39.0	41.0	36.0	41.0
35	39.033256635863395	40.0	39.0	41.0	35.0	41.0
36	38.99476315463759	40.0	39.0	41.0	35.0	41.0
37	38.95654108541306	40.0	39.0	41.0	35.0	41.0
38	38.91272992428828	40.0	38.0	41.0	35.0	41.0
39	38.836612903527865	40.0	38.0	41.0	35.0	41.0
40	38.78553586164287	40.0	38.0	41.0	35.0	41.0
41	38.732089761747524	40.0	38.0	41.0	35.0	41.0
42	38.699982617510145	40.0	38.0	41.0	35.0	41.0
43	38.64729392428351	40.0	38.0	41.0	35.0	41.0
44	38.609755441476935	40.0	38.0	41.0	35.0	41.0
45	38.7647557334551	40.0	38.0	41.0	35.0	41.0
46	38.8199145789377	40.0	38.0	41.0	35.0	41.0
47	38.78313370709943	40.0	38.0	41.0	35.0	41.0
48	38.75678941156101	40.0	38.0	41.0	35.0	41.0
49	38.72776453519205	40.0	38.0	41.0	35.0	41.0
50	38.691353922305204	40.0	38.0	41.0	35.0	41.0
51	38.630181280131	40.0	38.0	41.0	35.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	3.0
14	3.0
15	21.0
16	79.0
17	199.0
18	506.0
19	1135.0
20	2308.0
21	4342.0
22	8225.0
23	14384.0
24	23975.0
25	38392.0
26	57794.0
27	85446.0
28	120584.0
29	165973.0
30	223868.0
31	295027.0
32	381517.0
33	498445.0
34	668312.0
35	915787.0
36	1317461.0
37	2056579.0
38	4070449.0
39	1.8366278E7
40	51538.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.24974789695066	25.89113554432177	33.36823873071703	24.49087782801054
2	19.238677266383032	28.703505281611424	30.083181731981156	21.97463572002438
3	19.774937925693067	27.746054939060244	31.510481441790333	20.968525693456353
4	19.530651627040694	26.778825511842264	31.321866898860666	22.368655962256376
5	20.619889211738986	28.5771844690773	30.660607821916138	20.142318497267578
6	21.38436506379097	29.11236663202662	28.855941303413346	20.647327000769064
7	20.280877882135144	29.975254212529794	30.1591510807363	19.58471682459876
8	20.158686299175972	30.197402773400945	29.487056514543493	20.15685441287959
9	21.001449335319805	28.448710673819193	30.159072765894635	20.390767224966364
10	20.425750124239688	29.920015009209656	28.90024681092601	20.753988055624646
11	20.81005750659607	29.054874910580615	29.39668519108024	20.73838239174308
12	19.926299313901392	28.991461911988136	29.228310763644565	21.8539280104659
13	19.719731611632614	30.14103651735942	29.598553014125446	20.540678856882522
14	19.871778174232478	29.104491917993464	29.369907726389055	21.653822181385003
15	19.71177734748505	30.369436016264196	29.250690432772906	20.668096203477855
16	19.850531017188917	28.99311459317057	29.42544316411028	21.73091122553023
17	20.91658328689774	29.087211577929995	29.517977257029482	20.47822787814279
18	19.9087666144511	29.121533908538822	30.2549505620537	20.71474891495638
19	20.208317478834136	28.9173603063268	29.217176760172887	21.65714545466618
20	20.15374905915784	28.893763704033233	30.2247312481496	20.72775598865933
21	21.21195962972062	28.823614035858323	29.234773764596138	20.729652569824918
22	20.25322593304047	30.006661869031365	29.23564884782694	20.504463350101222
23	19.86789307708717	30.48202274297052	29.206022002725017	20.444062177217294
24	19.918876039012368	29.152025621892196	29.3933919639098	21.535706375185637
25	20.064425874746988	28.944348281763858	30.576831371291174	20.414394472197976
26	19.9368305677128	29.26601457643503	29.272388723548097	21.52476613230407
27	19.90108835497462	28.955291929638545	29.16153917265818	21.982080542728664
28	20.45219670577131	30.17220241934976	28.96729112537917	20.408309749499765
29	21.213750656099613	28.927258621314884	29.384453856980414	20.474536865605092
30	20.03806782303638	28.934933475798246	30.35805715859794	20.66894154256744
31	19.887805476829275	29.37462023686154	29.13035624570226	21.607218040606927
32	19.99708532589274	30.064992806100793	29.478503171836213	20.45941869617025
33	19.935233625941418	29.18793808428618	29.281939729238353	21.594888560534052
34	20.024178856116396	29.298106636552905	29.028284767065273	21.649429740265422
35	19.92781755093487	30.287689570863606	29.065453671919038	20.719039206282485
36	21.107981354938694	29.357115167252413	29.0461950308552	20.488708446953694
37	19.94653479809327	30.374234280891844	29.384804571271356	20.294426349743528
38	20.201442797733023	29.039677874031256	30.25162388377941	20.507255444456305
39	20.20468775617253	29.036245640970375	29.095148616883282	21.663917985973814
40	20.12441163847077	30.0518052677627	29.110106751641673	20.713676342124856
41	20.246294438796276	29.023186329427393	30.40963788798215	20.32088134379418
42	20.486944660519633	28.95650410718782	29.101539788961933	21.455011443330616
43	19.984132732079647	29.23731388946064	30.429150009865968	20.34940336859374
44	20.022667039172916	29.116085919553292	29.06665563448897	21.794591406784825
45	21.100760641740074	29.061189632989553	29.337129760267693	20.50091996500268
46	19.973094424480212	29.106607409796453	30.220367075976363	20.699931089746972
47	19.943387510214624	30.210953387329408	29.323806913620952	20.52185218883501
48	19.99670056167064	29.077745697067638	30.436280065450777	20.489273675810946
49	21.150971820807648	29.211328773066292	29.272044482948917	20.365654923177143
50	20.040206158713197	29.146186058698337	29.274016310257522	21.53959147233094
51	20.052762506340272	29.24029032590225	29.094334670488355	21.612612497269122
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.7406257249612
2	41.213312986407416
3	40.743463619149416
4	41.89930758929707
5	40.76220770900657
6	42.03169206456003
7	39.8655947067339
8	40.315540712055565
9	41.39221656028617
10	41.17973817986433
11	41.548439898339154
12	41.78022732436729
13	40.26041046851513
14	41.525600355617485
15	40.379873550962905
16	41.58144224271915
17	41.39481116504053
18	40.62351552940748
19	41.86546293350031
20	40.88150504781717
21	41.94161219954554
22	40.75768928314169
23	40.311955254304465
24	41.45458241419801
25	40.478820346944964
26	41.46159670001687
27	41.883168897703285
28	40.86050645527107
29	41.6882875217047
30	40.70700936560382
31	41.4950235174362
32	40.456504022062994
33	41.530122186475474
34	41.673608596381825
35	40.64685675721735
36	41.596689801892396
37	40.2409611478368
38	40.70869824218933
39	41.86860574214634
40	40.83808798059563
41	40.56717578259046
42	41.941956103850245
43	40.33353610067339
44	41.81725844595774
45	41.601680606742754
46	40.673025514227184
47	40.46523969904964
48	40.485974237481585
49	41.516626743984794
50	41.579797631044144
51	41.6653750036094
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2278.0
1	2912.5
2	3547.0
3	4986.0
4	6425.0
5	8641.5
6	10858.0
7	14542.0
8	18226.0
9	23058.5
10	27891.0
11	36813.0
12	45735.0
13	60137.0
14	74539.0
15	97825.0
16	121111.0
17	152473.5
18	183836.0
19	230334.0
20	276832.0
21	336632.0
22	396432.0
23	467434.5
24	538437.0
25	626202.5
26	813045.5
27	912123.0
28	1042012.0
29	1171901.0
30	1319904.0
31	1467907.0
32	1623893.5
33	1779880.0
34	1903888.0
35	2027896.0
36	2079116.5
37	2130337.0
38	2138953.5
39	2147570.0
40	2070546.0
41	1993522.0
42	1989766.0
43	1986010.0
44	1903697.0
45	1821384.0
46	1765367.0
47	1709350.0
48	1648174.0
49	1586998.0
50	1495930.0
51	1404862.0
52	1471061.0
53	1537260.0
54	1233247.0
55	929234.0
56	813814.0
57	698394.0
58	604240.0
59	510086.0
60	435906.5
61	361727.0
62	303669.5
63	245612.0
64	205936.5
65	166261.0
66	139090.5
67	111920.0
68	92663.5
69	73407.0
70	61987.0
71	50567.0
72	44041.0
73	37515.0
74	32919.0
75	23938.5
76	19554.0
77	16972.5
78	14391.0
79	12225.5
80	10060.0
81	8275.0
82	6490.0
83	5171.0
84	3852.0
85	3005.5
86	2159.0
87	1656.0
88	1153.0
89	818.5
90	484.0
91	352.5
92	221.0
93	147.0
94	73.0
95	48.5
96	24.0
97	15.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002329015291313335
2	0.009166241468151311
3	6.946185956548543E-4
4	0.004215381477552497
5	0.0
6	0.01941186575406041
7	0.0
8	0.0
9	0.0
10	0.0
11	1.3619972463820671E-5
12	3.9497920145079946E-4
13	0.0
14	0.0
15	0.005267524350382644
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	7.116435612346301E-4
42	0.0
43	0.0
44	0.0
45	0.0022813453876899623
46	3.404993115955168E-6
47	6.946185956548543E-4
48	0.0
49	0.0021042857456602935
50	0.0
51	0.002761449417039641
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9368635E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	95.58784874770005
#Duplication Level	Relative count
1	100.0
2	57.91489361702128
3	76.97872340425532
4	102.7872340425532
5	129.8404255319149
6	162.08510638297872
7	187.58510638297872
8	202.38297872340425
9	201.62765957446808
10++	906.4574468085107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC	319919	1.0893219926632614	TruSeq Adapter, Index 7 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA	63339	0.21566885897148438	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC	46402	0.15799849056655169	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	3554820	3.269973	17.756695	12
GTGTG	2979945	3.000077	3.5846097	6
CCAGG	2100400	2.918682	6.8517795	5
CCCAG	1997250	2.6631181	6.139478	34
CAGGG	1722745	2.4947817	6.7160892	6
CCCTG	1835130	2.40621	6.1448503	38
TCCAG	2509965	2.3660812	16.973505	25
GGAAG	2215585	2.3067224	19.192852	5
CTCCC	1715915	2.1589155	5.4748793	44
CCAGA	2201655	2.1105838	16.653774	33
CAGGC	1513480	2.1031075	5.7523127	36
CTCCA	2276655	2.0593607	16.093704	24
GGGGA	1356095	2.046579	5.9186006	19
CTGAA	3012790	2.041861	12.727611	19
AGAGC	1857810	1.8560147	17.915575	8
GCACA	1895730	1.8173133	17.082783	11
CACCA	1954580	1.79796	15.843722	31
GAAGA	2500665	1.7961019	13.4273205	6
GAGCA	1755960	1.754263	17.768845	9
AAGAG	2438455	1.7514197	13.329213	7
GTCTG	1737700	1.6786964	16.892147	17
TCTGA	2464000	1.6421267	12.109779	18
ACCAG	1702875	1.6324358	16.27397	32
CCAGT	1714615	1.6163247	16.239388	26
AGCAC	1675555	1.606246	16.979132	10
CAGTC	1570750	1.4807067	16.092299	27
CATCT	2308845	1.4765015	11.205574	39
ATGCC	1460410	1.3766918	15.780189	47
CAGAT	2028260	1.3746146	11.678244	34
ACTCC	1474165	1.3334639	15.4292345	23
TCACC	1467135	1.327105	15.192867	30
AGTCA	1856485	1.2581973	11.73255	28
GTCAC	1329165	1.2529705	15.818056	29
AACTC	1913420	1.2443452	11.434317	22
GAACT	1752270	1.1875677	11.845467	21
ATCTC	1837980	1.1753843	10.954461	40
TCATC	1739645	1.1124994	10.8123	38
GACCC	825930	1.1012889	5.079583	42
TGAAC	1623095	1.1000216	11.75596	20
CACGT	1155940	1.0896757	16.07545	14
AGATC	1456570	0.98716253	11.241073	35
GTATG	1394605	0.96859866	11.589314	45
ATCAT	2095185	0.96328956	7.975155	37
TATGC	1330025	0.8863918	11.080639	46
GATCA	1295185	0.8777869	11.170838	36
ACGTC	910060	0.8578908	15.742267	15
CGGGG	358685	0.7529328	6.2281513	18
CGGAA	741630	0.7409133	16.97433	4
TCTCG	786050	0.728653	14.927383	41
GGCGG	326735	0.68586504	6.2686553	38
ACACG	671445	0.64367074	15.834199	13
CGTCT	612470	0.5677478	15.218372	16
CTCGT	599245	0.55548847	14.742528	42
TCGGA	543405	0.53384155	16.49211	3
ATCGG	494890	0.48618034	16.427753	2
GATCG	479505	0.47106612	16.355238	1
CGTAT	542310	0.3614211	10.592573	44
TCGTA	493585	0.3289485	10.548565	43
>>END_MODULE