FastQCFastQC Report
Tue 12 Aug 2014
SV34_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SV34_GCCAAT_L001_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 28532577
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC 1676239 5.874825116567634 TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA 80538 0.28226682784383617 No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC 59834 0.20970415676088422 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACAC 4924435 3.9533322 70.12864 12
GGAAG 3777630 3.5418096 81.691 5
TCCAG 4081090 3.45047 71.98228 25
CTGAA 4389590 3.1098325 61.057396 19
CTCCA 3774405 3.0302906 68.22253 24
AGAGC 3323040 2.9585238 76.83801 8
GAAGA 3861290 2.8805995 64.973816 6
GAGCA 3231970 2.8774438 76.63215 9
GCACA 3367940 2.8473284 72.70277 11
GTCTG 3185125 2.8361144 75.80774 17
AAGAG 3724180 2.7783127 64.73795 7
CCAGT 3174175 2.683694 71.18995 26
AGCAC 3102580 2.6229873 72.62845 10
TCTGA 3672770 2.6021698 60.655716 18
CAGTC 3031480 2.5630481 71.063774 27
CCAGG 2407050 2.5574884 6.4306087 5
CGCCA 2531695 2.55431 83.07075 33
ATGCC 2871850 2.4280846 70.59932 47
ACTCC 2942535 2.3624213 67.72049 23
GCCAA 2786970 2.356164 69.740906 34
GTCAC 2766990 2.339428 70.73238 29
CACGT 2759185 2.332829 71.92244 14
TCACG 2679260 2.2652543 70.22277 30
AGTCA 3160680 2.2392037 59.613342 28
CCCAG 2215190 2.2349777 5.7783647 34
GAACT 3121665 2.211563 60.0372 21
CACGC 2180740 2.20022 83.154915 31
AACTC 3208350 2.158383 57.06558 22
CAGGG 1892860 2.1179385 6.2631536 6
ATCTC 3139405 2.1121411 56.187496 40
ACGTC 2495950 2.1102695 71.51194 15
TGAAC 2950760 2.0904844 59.972107 20
ACGCC 2066600 2.0850604 82.92755 32
CGGAA 2296180 2.044304 76.36502 4
GTATG 2732925 2.0390878 62.20599 45
CCCTG 1996645 2.0146148 5.7503295 38
TCTCG 2328815 1.9690917 69.969185 41
AATAT 4063280 1.9194387 39.53125 37
TATGC 2651930 1.8789011 58.993874 46
CCAAT 2783805 1.8727747 55.591568 35
ACACG 2169470 1.8341163 71.45614 13
TCGGA 2047355 1.8228946 76.223564 3
CTCCC 1900850 1.8212631 5.1590514 44
GGGGA 1517855 1.7885104 5.8859253 19
CAGGC 1675165 1.7798612 5.5528364 36
TATCT 3156450 1.7795669 46.86222 39
CGTCT 2103085 1.7782295 71.194084 16
CTCGT 2086175 1.7639315 69.794556 42
ATCGG 1980805 1.7636406 76.15033 2
AGGGA 1878035 1.7607977 5.2352858 7
GATCG 1970390 1.7543676 76.03596 1
CTTCC 2153825 1.7293187 5.0001607 2
CAATA 2816275 1.5875696 46.51569 36
ATATC 2791260 1.5735728 46.547127 38
CGTAT 1995580 1.4138751 58.545315 44
TCGTA 1952715 1.3835051 58.478886 43
CGGGG 574575 0.80797315 5.7639866 18
GGCGG 559675 0.78702056 5.7901244 38
Produced by FastQC (version 0.10.1)