##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV34_GCCAAT_L001_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	28532577	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.649674300362	34.0	31.0	34.0	31.0	34.0
2	32.81804472130225	34.0	31.0	34.0	31.0	34.0
3	32.889673372300024	34.0	31.0	34.0	31.0	34.0
4	36.31106282478446	37.0	37.0	37.0	35.0	37.0
5	36.228875120533274	37.0	37.0	37.0	35.0	37.0
6	36.20821950292117	37.0	37.0	37.0	35.0	37.0
7	36.19535606615554	37.0	37.0	37.0	35.0	37.0
8	36.212045024885064	37.0	37.0	37.0	35.0	37.0
9	37.980222221077334	39.0	38.0	39.0	35.0	39.0
10	37.96265230441681	39.0	38.0	39.0	35.0	39.0
11	37.96548100089242	39.0	38.0	39.0	35.0	39.0
12	37.96884406900926	39.0	38.0	39.0	35.0	39.0
13	37.94769119522572	39.0	38.0	39.0	35.0	39.0
14	39.45946358087459	41.0	39.0	41.0	36.0	41.0
15	39.43696109187754	41.0	39.0	41.0	36.0	41.0
16	39.44635502078904	41.0	39.0	41.0	36.0	41.0
17	39.415666940984686	41.0	39.0	41.0	36.0	41.0
18	39.397160130331024	41.0	39.0	41.0	36.0	41.0
19	39.43972747361726	41.0	39.0	41.0	36.0	41.0
20	39.41989905082881	41.0	39.0	41.0	36.0	41.0
21	39.41000821622246	41.0	39.0	41.0	36.0	41.0
22	39.385646203635936	41.0	39.0	41.0	36.0	41.0
23	39.36057412549872	41.0	39.0	41.0	36.0	41.0
24	39.3425123500061	41.0	39.0	41.0	36.0	41.0
25	39.30582498734692	41.0	39.0	41.0	36.0	41.0
26	39.24756961139543	41.0	39.0	41.0	36.0	41.0
27	39.20645846325062	41.0	39.0	41.0	36.0	41.0
28	39.125345880955656	41.0	39.0	41.0	36.0	41.0
29	39.10823845318984	41.0	39.0	41.0	36.0	41.0
30	39.08452450684703	40.0	39.0	41.0	36.0	41.0
31	39.035780644699564	40.0	39.0	41.0	35.0	41.0
32	38.93755499196585	40.0	39.0	41.0	35.0	41.0
33	38.93344593444889	40.0	39.0	41.0	35.0	41.0
34	38.81578207955069	40.0	38.0	41.0	35.0	41.0
35	38.81354691516297	40.0	38.0	41.0	35.0	41.0
36	38.78874228570381	40.0	38.0	41.0	35.0	41.0
37	38.673408293965174	40.0	38.0	41.0	35.0	41.0
38	38.64717936273334	40.0	38.0	41.0	35.0	41.0
39	38.558051801630114	40.0	38.0	41.0	34.0	41.0
40	38.513508962054146	40.0	38.0	41.0	34.0	41.0
41	38.478917764771126	40.0	38.0	41.0	34.0	41.0
42	38.420126545176764	40.0	38.0	41.0	34.0	41.0
43	38.29896030772124	40.0	38.0	41.0	34.0	41.0
44	38.307254826649555	40.0	38.0	41.0	34.0	41.0
45	38.47452573246363	40.0	38.0	41.0	34.0	41.0
46	38.49261046417223	40.0	38.0	41.0	34.0	41.0
47	38.47834361403809	40.0	38.0	41.0	34.0	41.0
48	38.45908135111666	40.0	38.0	41.0	34.0	41.0
49	38.42925922884568	40.0	38.0	41.0	34.0	41.0
50	38.39615846826594	40.0	38.0	41.0	34.0	41.0
51	38.29818736667214	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	14.0
15	41.0
16	122.0
17	327.0
18	807.0
19	1696.0
20	3435.0
21	6406.0
22	11224.0
23	19064.0
24	31019.0
25	47679.0
26	71100.0
27	101638.0
28	141626.0
29	192008.0
30	253306.0
31	329667.0
32	422652.0
33	545881.0
34	728607.0
35	1001740.0
36	1432208.0
37	2206153.0
38	4136630.0
39	1.6798116E7
40	49405.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.188198082525577	23.881510054042657	27.56498987484397	23.365301988587795
2	20.3049703671418	30.730380685443752	26.7799334629864	22.18471548442805
3	20.508894604973328	24.352422821644705	33.89327665661772	21.24540591676424
4	20.415568855601425	23.924857237099186	27.83162002197088	27.827953885328505
5	26.470368671986527	25.634667635290644	26.77232532699821	21.12263836572462
6	27.106219286463745	25.86990633962022	25.777573809586908	21.246300564329132
7	20.639401060759425	32.31896999699676	26.630388134937828	20.411240807305976
8	20.67716841699928	32.14509506098941	26.433644602098155	20.744091919913156
9	26.95112327218113	25.40225511351463	26.65097512923561	20.99564648506863
10	21.021294361178803	31.870938261202276	25.939262338624374	21.16850503899455
11	26.544339341565866	26.0371751261269	26.216507007622482	21.201978524684755
12	20.414150571525994	25.84427925332655	26.2813847225931	27.46018545255436
13	20.314610909487776	32.05032619380997	26.54842918675029	21.08663370995196
14	20.390401469870735	25.85017119203779	26.4786422901794	27.280785047912076
15	20.267278845380734	32.39471086675867	26.11055822519579	21.227452062664806
16	20.412236160792627	25.796537761030137	26.403426511387316	27.387799566789916
17	26.594250494794075	26.03780934333411	26.428180672218986	20.939759489652825
18	20.46972483417814	25.962726745642357	32.306072458859916	21.261475961319583
19	20.988405638929844	25.69105832957184	26.106912810574386	27.213623220923928
20	20.813395158803917	25.637771870378202	32.2089238557036	21.339909115114278
21	26.959296386022196	25.68859097445001	26.070915361062553	21.281197278465243
22	21.025380918099337	31.9316478143562	26.05250833109116	20.990462936453305
23	20.364252412251442	32.55876607289976	26.103464121029095	20.9735173938197
24	20.428214388065964	26.07967377079189	26.405413713594818	27.086698127547333
25	20.687647666735465	25.852989023739426	32.522856242532875	20.93650706699223
26	20.45306668233998	26.243731857798895	26.269604038920143	27.03359742094098
27	20.476352346302264	25.965947625410774	25.988160831038854	27.569539197248115
28	21.168950144250903	32.08650238637751	25.908602647422978	20.83594482194861
29	26.945967761692184	25.86976984238052	26.330306582542473	20.853955813384818
30	20.570017212255312	25.76602877475806	32.3827322011608	21.28122181182583
31	20.48596942365213	26.384336052085306	26.05306208408725	27.076632440175313
32	20.527592022269843	31.936407286309958	26.53098947213916	21.005011219281034
33	20.543331925468912	26.169662137422776	26.24297482838651	27.044031108721793
34	26.621233686673307	26.217824629019663	25.988914355685434	21.172027328621596
35	20.479306863870026	26.046872667687886	25.976539027652496	27.497281440789596
36	20.437891046434398	26.398845782489254	25.98437918874275	27.178883982333595
37	20.431582467997895	32.46128802175843	26.315225575313438	20.791903934930236
38	20.79878729495762	32.13542541215257	26.040378336664087	21.02540895622572
39	20.772957171025947	26.054751381201914	32.127045517129424	21.045245930642718
40	20.598514462959304	31.94183266376535	26.11766893680862	21.341983936466725
41	20.823167692798794	25.89435556533158	32.429157349185076	20.85331939268455
42	21.151874925282772	25.81512353405723	26.111868549412836	26.921132991247166
43	20.53562144071319	26.103166215936263	32.434476563403294	20.92673577994725
44	20.630316707810863	25.919824907508353	26.04570908544293	27.40414929923785
45	26.63754119328806	25.94376115985607	26.39844785954723	21.020249787308643
46	20.565651715626785	26.067384384339654	32.15054372064211	21.21642017939145
47	20.48613577848817	32.120327491564936	26.429540677599523	20.96399605234737
48	20.599328290582665	26.011040853794626	32.38052182880368	21.00910902681903
49	26.705147211232415	26.14671095192295	26.29998800644638	20.848153830398253
50	20.641661634699172	26.11521209598418	26.218409924907938	27.024716344408713
51	20.63238951438559	26.12729425475716	25.98764312707212	27.25267310378513
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	48.55350007111337
2	42.489685851569845
3	41.75430052173757
4	48.243522740929926
5	47.593007037711146
6	48.35251985079287
7	41.050641868065405
8	41.42126033691243
9	47.946769757249754
10	42.18979940017335
11	47.74631786625062
12	47.87433602408035
13	41.40124461943974
14	47.671186517782814
15	41.49473090804554
16	47.80003572758255
17	47.534009984446904
18	41.73120079549772
19	48.202028859853776
20	42.153304273918195
21	48.24049366448744
22	42.01584385455264
23	41.337769806071144
24	47.5149125156133
25	41.6241547337277
26	47.486664103280965
27	48.045891543550376
28	42.004894966199515
29	47.79992357507701
30	41.85123902408114
31	47.56260186382744
32	41.53260324155087
33	47.5873630341907
34	47.7932610152949
35	47.976588304659614
36	47.616775028768
37	41.22348640292813
38	41.82419625118334
39	41.818203101668665
40	41.940498399426026
41	41.676487085483345
42	48.07300791652994
43	41.46235722066044
44	48.034466007048714
45	47.6577909805967
46	41.782071895018234
47	41.45013183083554
48	41.608437317401695
49	47.55330104163067
50	47.666377979107885
51	47.88506261817072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	282.5
2	374.0
3	571.5
4	769.0
5	1131.5
6	1494.0
7	2214.0
8	2934.0
9	4439.5
10	5945.0
11	8810.5
12	11676.0
13	17177.0
14	22678.0
15	31515.0
16	40352.0
17	55189.0
18	70026.0
19	91379.5
20	112733.0
21	144680.0
22	176627.0
23	218925.5
24	261224.0
25	317401.0
26	450960.5
27	528343.0
28	639046.0
29	749749.0
30	896441.5
31	1043134.0
32	1205914.0
33	1368694.0
34	1515431.0
35	1662168.0
36	1735282.0
37	1808396.0
38	1835538.0
39	1862680.0
40	1809374.0
41	1756068.0
42	1787268.0
43	1818468.0
44	1769690.0
45	1720912.0
46	1709974.5
47	1699037.0
48	1683725.0
49	1668413.0
50	1627295.0
51	1586177.0
52	2363196.5
53	3140216.0
54	2153015.5
55	1165815.0
56	1036666.5
57	907518.0
58	809189.5
59	710861.0
60	626923.5
61	542986.0
62	476123.0
63	409260.0
64	361324.0
65	313388.0
66	279753.0
67	246118.0
68	214007.0
69	181896.0
70	162542.5
71	143189.0
72	128417.0
73	113645.0
74	102068.0
75	80406.5
76	70322.0
77	61312.5
78	52303.0
79	44655.5
80	37008.0
81	30371.5
82	23735.0
83	19324.0
84	14913.0
85	11589.0
86	8265.0
87	6435.5
88	4606.0
89	3302.0
90	1998.0
91	1380.5
92	763.0
93	525.5
94	288.0
95	199.0
96	110.0
97	73.0
98	36.0
99	21.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002351697850495593
2	0.009084352948561218
3	7.885723045626058E-4
4	0.00413562364170611
5	1.7523828990280127E-5
6	0.018410534737188303
7	0.0
8	0.0
9	0.0
10	0.0
11	1.4019063192224103E-5
12	3.644956429978267E-4
13	0.0
14	0.0
15	0.005169529552132637
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	6.624007358325888E-4
42	0.0
43	0.0
44	0.0
45	0.002183469092188904
46	7.009531596112052E-6
47	6.238483120539725E-4
48	3.504765798056026E-6
49	0.002043278460266663
50	0.0
51	0.0026741363039167474
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8532577E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	46.80957713914128
#Duplication Level	Relative count
1	100.0
2	42.46309015426151
3	12.178763360982112
4	3.3475680677833717
5	1.2692402387360235
6	0.8455941657398525
7	0.625705723042441
8	0.5119410461256335
9	0.5127900362518784
10++	8.043332456043537
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	1676239	5.874825116567634	TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA	80538	0.28226682784383617	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC	59834	0.20970415676088422	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	4924435	3.9533322	70.12864	12
GGAAG	3777630	3.5418096	81.691	5
TCCAG	4081090	3.45047	71.98228	25
CTGAA	4389590	3.1098325	61.057396	19
CTCCA	3774405	3.0302906	68.22253	24
AGAGC	3323040	2.9585238	76.83801	8
GAAGA	3861290	2.8805995	64.973816	6
GAGCA	3231970	2.8774438	76.63215	9
GCACA	3367940	2.8473284	72.70277	11
GTCTG	3185125	2.8361144	75.80774	17
AAGAG	3724180	2.7783127	64.73795	7
CCAGT	3174175	2.683694	71.18995	26
AGCAC	3102580	2.6229873	72.62845	10
TCTGA	3672770	2.6021698	60.655716	18
CAGTC	3031480	2.5630481	71.063774	27
CCAGG	2407050	2.5574884	6.4306087	5
CGCCA	2531695	2.55431	83.07075	33
ATGCC	2871850	2.4280846	70.59932	47
ACTCC	2942535	2.3624213	67.72049	23
GCCAA	2786970	2.356164	69.740906	34
GTCAC	2766990	2.339428	70.73238	29
CACGT	2759185	2.332829	71.92244	14
TCACG	2679260	2.2652543	70.22277	30
AGTCA	3160680	2.2392037	59.613342	28
CCCAG	2215190	2.2349777	5.7783647	34
GAACT	3121665	2.211563	60.0372	21
CACGC	2180740	2.20022	83.154915	31
AACTC	3208350	2.158383	57.06558	22
CAGGG	1892860	2.1179385	6.2631536	6
ATCTC	3139405	2.1121411	56.187496	40
ACGTC	2495950	2.1102695	71.51194	15
TGAAC	2950760	2.0904844	59.972107	20
ACGCC	2066600	2.0850604	82.92755	32
CGGAA	2296180	2.044304	76.36502	4
GTATG	2732925	2.0390878	62.20599	45
CCCTG	1996645	2.0146148	5.7503295	38
TCTCG	2328815	1.9690917	69.969185	41
AATAT	4063280	1.9194387	39.53125	37
TATGC	2651930	1.8789011	58.993874	46
CCAAT	2783805	1.8727747	55.591568	35
ACACG	2169470	1.8341163	71.45614	13
TCGGA	2047355	1.8228946	76.223564	3
CTCCC	1900850	1.8212631	5.1590514	44
GGGGA	1517855	1.7885104	5.8859253	19
CAGGC	1675165	1.7798612	5.5528364	36
TATCT	3156450	1.7795669	46.86222	39
CGTCT	2103085	1.7782295	71.194084	16
CTCGT	2086175	1.7639315	69.794556	42
ATCGG	1980805	1.7636406	76.15033	2
AGGGA	1878035	1.7607977	5.2352858	7
GATCG	1970390	1.7543676	76.03596	1
CTTCC	2153825	1.7293187	5.0001607	2
CAATA	2816275	1.5875696	46.51569	36
ATATC	2791260	1.5735728	46.547127	38
CGTAT	1995580	1.4138751	58.545315	44
TCGTA	1952715	1.3835051	58.478886	43
CGGGG	574575	0.80797315	5.7639866	18
GGCGG	559675	0.78702056	5.7901244	38
>>END_MODULE