##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV33_ACAGTG_L001_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	26524278	
Filtered Sequences	0	
Sequence length	51	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68547645293116	34.0	31.0	34.0	31.0	34.0
2	32.8787156430799	34.0	31.0	34.0	31.0	34.0
3	32.932379196146265	34.0	31.0	34.0	31.0	34.0
4	36.33489869168164	37.0	37.0	37.0	35.0	37.0
5	36.24805625246425	37.0	37.0	37.0	35.0	37.0
6	36.23517884256831	37.0	37.0	37.0	35.0	37.0
7	36.22460977071648	37.0	37.0	37.0	35.0	37.0
8	36.227258966294954	37.0	37.0	37.0	35.0	37.0
9	38.016888527559544	39.0	38.0	39.0	35.0	39.0
10	38.00578221205493	39.0	38.0	39.0	35.0	39.0
11	38.0045959780696	39.0	38.0	39.0	35.0	39.0
12	37.99661593050714	39.0	38.0	39.0	35.0	39.0
13	37.9672895903142	39.0	38.0	39.0	35.0	39.0
14	39.47666089158016	41.0	39.0	41.0	36.0	41.0
15	39.446715231984825	41.0	39.0	41.0	36.0	41.0
16	39.445708493931484	41.0	39.0	41.0	36.0	41.0
17	39.43034102568221	41.0	39.0	41.0	36.0	41.0
18	39.44251093281408	41.0	39.0	41.0	36.0	41.0
19	39.45965126741621	41.0	39.0	41.0	36.0	41.0
20	39.429309442466256	41.0	39.0	41.0	36.0	41.0
21	39.414665801647836	41.0	39.0	41.0	36.0	41.0
22	39.36661593578532	41.0	39.0	41.0	36.0	41.0
23	39.334333435956296	41.0	39.0	41.0	36.0	41.0
24	39.32076989993846	41.0	39.0	41.0	36.0	41.0
25	39.29148175117151	41.0	39.0	41.0	36.0	41.0
26	39.22464117590684	41.0	39.0	41.0	36.0	41.0
27	39.174680909316365	41.0	39.0	41.0	36.0	41.0
28	39.10360564762592	41.0	39.0	41.0	36.0	41.0
29	39.08639880791477	41.0	39.0	41.0	36.0	41.0
30	39.07222681047153	40.0	39.0	41.0	36.0	41.0
31	39.00804263173535	40.0	39.0	41.0	35.0	41.0
32	38.93305872453908	40.0	39.0	41.0	35.0	41.0
33	38.88634250477996	40.0	38.0	41.0	35.0	41.0
34	38.805817900114	40.0	38.0	41.0	35.0	41.0
35	38.764271057632556	40.0	38.0	41.0	35.0	41.0
36	38.7109699649506	40.0	38.0	41.0	35.0	41.0
37	38.65389745952746	40.0	38.0	41.0	35.0	41.0
38	38.55877837654997	40.0	38.0	41.0	34.0	41.0
39	38.477984584537985	40.0	38.0	41.0	34.0	41.0
40	38.398347921100815	40.0	38.0	41.0	34.0	41.0
41	38.34516294845047	40.0	38.0	41.0	34.0	41.0
42	38.30510994493422	40.0	38.0	41.0	34.0	41.0
43	38.262453779137736	40.0	38.0	41.0	33.0	41.0
44	38.196260874659814	40.0	38.0	41.0	33.0	41.0
45	38.377193716639525	40.0	38.0	41.0	34.0	41.0
46	38.424056632191835	40.0	38.0	41.0	34.0	41.0
47	38.386225102903836	40.0	38.0	41.0	34.0	41.0
48	38.349379312040085	40.0	38.0	41.0	34.0	41.0
49	38.309779440556305	40.0	38.0	41.0	34.0	41.0
50	38.259794932024164	40.0	38.0	41.0	34.0	41.0
51	38.1735815768482	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	4.0
14	19.0
15	82.0
16	178.0
17	476.0
18	1080.0
19	2369.0
20	4351.0
21	7650.0
22	12981.0
23	21064.0
24	32229.0
25	48410.0
26	69213.0
27	98100.0
28	134853.0
29	180380.0
30	237233.0
31	307037.0
32	390557.0
33	503810.0
34	674588.0
35	932483.0
36	1362093.0
37	2115088.0
38	3885318.0
39	1.5460927E7
40	41700.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.61911982419603	23.66034471974484	28.24326516657805	26.47727028948108
2	21.761947888985077	26.60834617809322	26.471511780479783	25.158194152441908
3	22.421458812685547	24.60262424965815	29.23542636375245	23.740490573903852
4	22.309381045172934	23.66629355974318	27.750291178735857	26.274034216348024
5	24.21705023040215	25.506800845640456	27.559282958723657	22.716865965233733
6	24.862724765124334	25.854494925403525	25.930918367154547	23.35186194231759
7	22.953620075916863	27.772299777584898	26.824764089714336	22.449316056783903
8	22.682328996853375	28.021705246793143	26.30733247479913	22.988633281554353
9	24.254677167838462	25.65302625767985	26.84549980964609	23.246796764835597
10	22.542042426187813	27.760156185966682	26.133963005515177	23.56383838233033
11	24.2746362408055	25.693528773902912	26.412055400716277	23.61977958457531
12	22.50018228640614	25.764194757435423	26.182013296992174	25.55360965916627
13	22.39030973811992	27.870417434171063	26.343993227638467	23.395279600070545
14	22.5064825515703	25.92145203726186	26.2107605718806	25.361304839287236
15	22.436686255514758	27.9104739396282	26.271460971445293	23.38137883341174
16	22.530732033497763	25.884308707667746	26.25732545858553	25.327633800248968
17	24.486502516675476	25.856010105157246	26.340298499359722	23.31718887880756
18	22.593919427326163	25.87949425051268	28.20739927397835	23.3191870481828
19	22.603001672656273	25.836997335045275	26.280545694778194	25.279455297520258
20	22.639899943742105	25.825434343585147	28.19764217521774	23.337023537455007
21	24.553316776426488	25.855919622015726	26.282883175934142	23.30788042562365
22	22.620272642293976	27.829922458209793	26.259817515108235	23.289987384387995
23	22.490429334212227	27.965032639154213	26.30472354421862	23.239814482414943
24	22.59402876112217	25.938281901584652	26.281228088470492	25.186461248822685
25	22.572177082445	25.955315352975866	28.267743989110656	23.204763575468483
26	22.631432229748157	26.056569758468072	26.204419211712377	25.10757880007139
27	22.621365980254016	26.041851167447422	26.2022325357923	25.13455031650626
28	22.78391140373359	28.01317721070485	26.10408848828986	23.098822897271702
29	24.64667652782104	25.998901836272413	26.24089145800689	23.11353017789966
30	22.69375626360122	25.984130463419213	28.14744665245931	23.174666620520266
31	22.624547970730816	26.063012912170503	26.20663227854873	25.10580683854995
32	22.68128844072589	27.96249911119164	26.232962872731164	23.123249575351306
33	22.63750968075361	26.064355078769726	26.198345530837823	25.09978970963884
34	22.668609490520346	28.029935442540605	26.186989896577018	23.114465170362035
35	22.623235965178772	26.14411219788904	26.188920203596115	25.04373163333607
36	22.716558015264354	28.111883007710897	26.1555281542442	23.016030822780547
37	24.64010895979902	26.15093236468114	26.233958187287886	22.97500048823195
38	22.79292201657666	26.089761990882465	28.091381035894734	23.025934956646132
39	24.710459602331117	26.106320405780696	26.170770793459486	23.0124491984287
40	22.760653466231958	27.989342443176024	26.172135580844085	23.077868509747937
41	22.719400979125236	26.06349384204936	28.176720447725923	23.04038473109948
42	22.7927674412099	26.0223294296644	26.196385062771547	24.988518066354153
43	22.683339391933686	26.065542670002177	28.19012076407886	23.06099717398528
44	22.74653432602388	26.075952001407916	26.169696306153934	25.00781736641427
45	24.6494731224491	26.100287987561323	26.2541842272095	22.996054662780082
46	22.73331624710011	26.161066476531424	28.11532513721957	22.990292139148895
47	22.703942656221525	28.10302540731074	26.237608532437186	22.955423404030554
48	22.77851634642044	26.124104113220348	28.13424742419002	22.963132116169195
49	24.716043693885823	26.117404913663854	26.185125452467794	22.98142593998253
50	22.77802245927297	26.093671616622327	26.141073472386317	24.987232451718384
51	22.765278403531955	26.097215493401148	26.13681049482441	25.000695608242484
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	48.09639011367711
2	46.920142041426985
3	46.1619493865894
4	48.583415261520955
5	46.93391619563588
6	48.214586707441924
7	45.402936132700766
8	45.67096227840773
9	47.50147393267406
10	46.105880808518144
11	47.89441582538081
12	48.053791945572414
13	45.78558933819047
14	47.867787390857536
15	45.8180650889265
16	47.85836583374673
17	47.80369139548304
18	45.91310647550896
19	47.88245697017653
20	45.97692348119711
21	47.861197202050136
22	45.91026002668197
23	45.73024381662717
24	47.780490009944856
25	45.77694065791348
26	47.73901102981955
27	47.755916296760276
28	45.88273430100529
29	47.7602067057207
30	45.868422884121486
31	47.73035480928077
32	45.804538016077196
33	47.73729939039246
34	45.783074660882384
35	47.666967598514844
36	45.732588838044904
37	47.61510944803097
38	45.81885697322279
39	47.72290880075982
40	45.83852197597989
41	45.75978571022471
42	47.78128550756405
43	45.74433656591897
44	47.75435169243815
45	47.64552778522918
46	45.723608386249005
47	45.659366060252076
48	45.74164846258964
49	47.69746963386835
50	47.76525491099136
51	47.76597401177444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	198.5
2	278.0
3	366.0
4	454.0
5	661.5
6	869.0
7	1299.0
8	1729.0
9	2622.5
10	3516.0
11	5342.5
12	7169.0
13	10534.5
14	13900.0
15	20259.0
16	26618.0
17	36838.5
18	47059.0
19	63725.5
20	80392.0
21	103729.0
22	127066.0
23	160662.0
24	194258.0
25	238747.0
26	342386.0
27	401536.0
28	479935.5
29	558335.0
30	657504.5
31	756674.0
32	867397.5
33	978121.0
34	1089944.0
35	1201767.0
36	1288834.0
37	1375901.0
38	1450053.0
39	1524205.0
40	1563969.0
41	1603733.0
42	1660040.5
43	1716348.0
44	1748894.5
45	1781441.0
46	1804249.0
47	1827057.0
48	1824734.5
49	1822412.0
50	1782157.0
51	1741902.0
52	1912112.0
53	2082322.0
54	1710788.0
55	1339254.0
56	1214916.5
57	1090579.0
58	977568.5
59	864558.0
60	766479.5
61	668401.0
62	587735.0
63	507069.0
64	447196.5
65	387324.0
66	346343.0
67	305362.0
68	273227.0
69	241092.0
70	221496.0
71	201900.0
72	187706.0
73	173512.0
74	161629.5
75	137232.0
76	124717.0
77	112886.0
78	101055.0
79	89396.5
80	77738.0
81	66327.0
82	54916.0
83	45879.0
84	36842.0
85	29443.0
86	22044.0
87	16521.0
88	10998.0
89	8152.0
90	5306.0
91	3756.0
92	2206.0
93	1510.5
94	815.0
95	559.0
96	303.0
97	205.5
98	108.0
99	61.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002431734428360312
2	0.009191579126112312
3	7.577963102332135E-4
4	0.003958637441516788
5	1.1310392690047963E-5
6	0.01857920505885212
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	3.1669099532134293E-4
13	0.0
14	0.0
15	0.00514245854307514
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	7.314053939564349E-4
42	0.0
43	0.0
44	0.0
45	0.0021225836948323344
46	0.0
47	6.786235614028778E-4
48	0.0
49	0.002137664218419065
50	0.0
51	0.0027446552927849724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.6524278E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.835067037643833
#Duplication Level	Relative count
1	100.0
2	6.352357596080591
3	1.0154429633584134
4	0.46823821201959703
5	0.29417816408357106
6	0.24579725619080986
7	0.2052017817520562
8	0.17294784315688205
9	0.16571851209244645
10++	2.3005955856592317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC	499165	1.881917389042597	TruSeq Adapter, Index 5 (100% over 51bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	4075100	3.4556932	23.990685	12
ACACA	3946095	3.0293443	21.351763	32
CACAG	3023850	2.6598623	23.622	33
GGAAG	2698025	2.5535765	26.216307	5
CAGTG	2647200	2.3815393	23.615805	35
TCCAG	2718525	2.3577752	23.397112	25
CTCCA	2645135	2.2116423	22.48026	24
GAAGA	2533400	2.0926127	22.72282	6
CTGAA	2661965	2.0900445	21.274727	19
AAGAG	2487540	2.0547318	22.649513	7
AGAGC	2246485	2.0497665	24.795954	8
GAGCA	2113775	1.9286777	24.591618	9
TCTGA	2469980	1.9121274	20.84612	18
GCACA	2152450	1.8933548	23.683756	11
GTCTG	2062235	1.8292763	23.552464	17
TCACA	2362025	1.787869	19.871096	30
AGCAC	1990675	1.751053	23.59853	10
CCAGT	2003050	1.7372439	22.706	26
AGTGA	2092940	1.704559	20.984344	36
ACAGT	2145695	1.6846946	20.264755	34
CAGTC	1883405	1.633476	22.578825	27
AGTCA	1961480	1.5400583	20.476751	28
GAACT	1881320	1.4771203	20.64963	21
AACTC	1934965	1.4646178	19.928726	22
ACTCC	1750890	1.463949	21.831879	23
ATGCC	1639040	1.4215384	22.268293	47
GTCAC	1616985	1.40241	22.317745	29
ATCTC	1869345	1.3951175	19.31232	40
TGAAC	1710110	1.3426946	20.54391	20
GTGAT	1659100	1.3322874	20.328472	37
GATCT	1573655	1.2182401	19.700016	39
GTATG	1431885	1.1498296	20.490898	45
TGATC	1404685	1.0874325	19.384748	38
TATGC	1359810	1.0526927	19.689964	46
CACGT	1174640	1.0187646	22.407228	14
CGGAA	966590	0.88194853	23.79601	4
TCTCG	981210	0.8390757	21.376343	41
ACACG	943105	0.8295813	22.516956	13
ACGTC	932100	0.80840975	22.159386	15
CGTCT	880690	0.7531166	21.753164	16
TCGGA	820835	0.7384599	23.32436	3
CTCGT	839470	0.7178676	21.265642	42
ATCGG	730995	0.6576358	23.206846	2
GATCG	722590	0.6500743	23.146025	1
CGTAT	704185	0.5451426	19.183174	44
TCGTA	679895	0.52633864	19.171896	43
>>END_MODULE