##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV32_TGACCA_L001_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	31356637	
Filtered Sequences	0	
Sequence length	51	
%GC	53	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61007703090099	34.0	31.0	34.0	31.0	34.0
2	32.73426978154577	34.0	31.0	34.0	31.0	34.0
3	32.83674409982167	34.0	31.0	34.0	31.0	34.0
4	36.28898746380232	37.0	37.0	37.0	35.0	37.0
5	36.22023860530707	37.0	37.0	37.0	35.0	37.0
6	36.1790231522596	37.0	37.0	37.0	35.0	37.0
7	36.07873232706683	37.0	35.0	37.0	35.0	37.0
8	36.126258629074286	37.0	36.0	37.0	35.0	37.0
9	37.89885222704208	39.0	38.0	39.0	35.0	39.0
10	37.81598763285744	39.0	38.0	39.0	35.0	39.0
11	37.86846012855269	39.0	38.0	39.0	35.0	39.0
12	37.89171501395382	39.0	38.0	39.0	35.0	39.0
13	37.87322294798387	39.0	38.0	39.0	35.0	39.0
14	39.39592941679301	41.0	39.0	41.0	36.0	41.0
15	39.348514829571805	41.0	39.0	41.0	36.0	41.0
16	39.39125404296386	41.0	39.0	41.0	36.0	41.0
17	39.27665294591381	41.0	39.0	41.0	36.0	41.0
18	39.15944292750527	40.0	39.0	41.0	36.0	41.0
19	39.239324931433174	41.0	39.0	41.0	36.0	41.0
20	39.21914623688758	41.0	39.0	41.0	36.0	41.0
21	39.195898431327315	41.0	39.0	41.0	36.0	41.0
22	39.02496683556977	40.0	39.0	41.0	35.0	41.0
23	39.084950787292655	40.0	39.0	41.0	35.0	41.0
24	39.08417697982089	41.0	39.0	41.0	35.0	41.0
25	38.99158500320044	40.0	39.0	41.0	35.0	41.0
26	38.97600504799032	41.0	39.0	41.0	35.0	41.0
27	38.92417257628744	41.0	39.0	41.0	35.0	41.0
28	38.75446142390844	40.0	38.0	41.0	35.0	41.0
29	38.69583712054325	40.0	38.0	41.0	35.0	41.0
30	38.48138367006641	40.0	38.0	41.0	34.0	41.0
31	38.491940478183295	40.0	38.0	41.0	34.0	41.0
32	38.379903750520185	40.0	38.0	41.0	34.0	41.0
33	38.38050815207001	40.0	38.0	41.0	34.0	41.0
34	38.29214685873361	40.0	38.0	41.0	34.0	41.0
35	38.18916282380665	40.0	38.0	41.0	33.0	41.0
36	38.039552200703156	40.0	37.0	41.0	33.0	41.0
37	38.039003991403796	40.0	37.0	41.0	33.0	41.0
38	37.94302105802991	40.0	37.0	41.0	33.0	41.0
39	37.734274852242606	40.0	37.0	41.0	33.0	41.0
40	37.58490908958126	40.0	36.0	41.0	32.0	41.0
41	37.50871172823795	40.0	36.0	41.0	32.0	41.0
42	37.36020760134449	40.0	36.0	41.0	32.0	41.0
43	37.106508902724485	39.0	35.0	41.0	31.0	41.0
44	37.14963578524062	40.0	35.0	41.0	31.0	41.0
45	37.30982748564522	40.0	35.0	41.0	32.0	41.0
46	37.1996887293749	39.0	35.0	41.0	32.0	41.0
47	37.220270432699785	39.0	35.0	41.0	32.0	41.0
48	37.18011552067908	39.0	35.0	41.0	32.0	41.0
49	37.12542151762002	39.0	35.0	41.0	32.0	41.0
50	37.062178192132016	39.0	35.0	41.0	32.0	41.0
51	36.87591587069748	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	15.0
14	70.0
15	203.0
16	593.0
17	1557.0
18	3555.0
19	6683.0
20	12195.0
21	20148.0
22	31430.0
23	46704.0
24	66654.0
25	93259.0
26	125722.0
27	168511.0
28	221870.0
29	287124.0
30	367408.0
31	465072.0
32	587657.0
33	750042.0
34	1006541.0
35	1416721.0
36	2163508.0
37	3346495.0
38	5158430.0
39	1.496278E7
40	45685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.83383318013004	18.25777768876399	20.40937313920829	24.49901599189768
2	21.18267738183289	35.819212985776154	19.669806010074094	23.328303622316863
3	21.37890486185097	17.977483441892275	38.21732545687417	22.426286239382588
4	21.032295644047174	17.69693778137864	20.90067253410222	40.37009404047196
5	38.81848805464974	19.08711444684309	20.01412715318059	22.080270345326582
6	39.278353234937406	19.263389034476063	19.13314364984932	22.32511408073721
7	21.71172246564579	37.15741263962714	19.81798303179005	21.312881862937026
8	21.626314709705635	36.88320593818782	19.670087707428575	21.820391644677965
9	39.17569030122714	19.079858595805412	19.74231802983209	22.00213307313536
10	21.91650845720477	36.584069905200614	19.29462652515957	22.20479511243505
11	38.59373745281461	19.717904032684118	19.379602415233727	22.308756099267544
12	21.285424548604016	19.409784968375302	19.600574732438282	39.7042157505824
13	21.19930782117993	36.613926423295965	19.902373459245645	22.284392296278458
14	21.35943022206112	19.283840929752767	19.82950531334084	39.52722353484527
15	21.249119440483362	36.91282530949387	19.300075420034226	22.537979829988547
16	21.437034207463	19.156553682717952	19.675167971616343	39.731244138202705
17	38.63055531114513	19.427025927557217	19.80536369381704	22.13705506748061
18	21.489495190444053	19.38860025072204	36.55468537649621	22.5672191823377
19	22.039946439409302	19.092308910550578	19.581063492236108	39.28668115780401
20	21.979359585021825	19.097701708253982	36.461311205024955	22.46162750169924
21	38.946874309257076	19.335916029515538	19.351561840002166	22.365647821225217
22	22.12405622452433	36.517468375195975	19.282775764505615	22.07569963577408
23	21.331605171817372	37.06462526577707	19.30336151800973	22.300408044395834
24	21.392858551763698	19.48191063984317	19.915605107779893	39.20962570061324
25	21.755292189018867	19.082288703345323	36.72789591562386	22.434523192011948
26	21.477497730384798	19.502923735093148	19.65643190626597	39.36314662825608
27	21.536812126887202	19.45177029028974	19.202926640379196	39.80849094244386
28	22.568147215532075	36.219104108645325	19.187561472233135	22.025187203589468
29	38.946545830153916	19.25426505399798	19.868941940425564	21.930247175422544
30	21.687950783752736	19.120290227552143	36.561018963864015	22.630740024831105
31	21.491804111518718	20.132551204390957	19.349093463052174	39.02655122103815
32	21.55449897257796	36.17938046098502	19.862216091604466	22.403904474832554
33	21.550394578347163	19.609035241885156	19.844723782081605	38.99584639768608
34	21.87674654013439	19.50451829384637	36.24927953849132	22.369455627527916
35	38.569359335313926	19.528417540439687	19.321051552817988	22.581171571428403
36	21.702489970464626	36.62790113620922	19.139660927286304	22.529947966039853
37	21.558370561230785	19.70682953022035	19.505436759688227	39.22936314886064
38	21.947882995233194	19.58817203515798	19.12583291377835	39.33811205583048
39	21.93820083448362	36.53772564959692	19.159717287284348	22.364356228635103
40	21.615851215166984	36.262217788214976	19.576059766868497	22.545871229749544
41	21.920589322039703	19.303493591969854	36.49849514157189	22.27742194441856
42	22.300848142611724	19.10866270512364	19.732926078775602	38.85756307348904
43	21.626317898823142	19.43576729864239	36.4456558271858	22.492258975348665
44	22.02854215520625	19.123214648305556	19.350279814764573	39.49796338172362
45	38.526747229084904	19.189467449858125	19.95209079276975	22.331694528287223
46	21.76906557537603	19.60766323348556	36.14318220964604	22.48008898149237
47	21.652035912115906	36.112875324450926	20.111411255047887	22.12367750838528
48	22.054657838466543	19.330280858881647	36.421947927642876	22.193113375008934
49	38.59434485229907	19.450651833821393	19.640577219069858	22.314426094809676
50	21.829573113979027	19.47699302064823	19.424729762952577	39.268704102420166
51	22.0711591405411	19.456004515914533	19.165534897479013	39.30730144606536
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	61.332849172027714
2	44.510981004149755
3	43.80519110123356
4	61.40238968451913
5	60.89875839997633
6	61.60346731567462
7	43.02460432858281
8	43.4467063543836
9	61.1778233743625
10	44.12130356963982
11	60.90249355208215
12	60.98964029918641
13	43.48370011745838
14	60.8866537569064
15	43.787099270471906
16	61.168278345665705
17	60.76761037862575
18	44.056714372781755
19	61.32662759721331
20	44.440987086721066
21	61.31252213048229
22	44.19975586029841
23	43.6320132162132
24	60.60248425237694
25	44.18981538103082
26	60.84064435864088
27	61.34530306933106
28	44.59333441912154
29	60.87679300557646
30	44.318690808583845
31	60.51835533255687
32	43.95840344741051
33	60.54624097603324
34	44.246202167662304
35	61.150530906742325
36	44.23243793650448
37	60.787733710091416
38	61.285995051063665
39	44.302557063118726
40	44.16172244491652
41	44.198011266458266
42	61.15841121610076
43	44.11857687417181
44	61.52650553692987
45	60.85844175737213
46	44.2491545568684
47	43.77571342050119
48	44.247771213475474
49	60.90877094710875
50	61.0982772163992
51	61.37846058660645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	194.0
1	244.5
2	295.0
3	463.0
4	631.0
5	911.5
6	1192.0
7	1790.0
8	2388.0
9	3455.0
10	4522.0
11	6435.0
12	8348.0
13	11867.5
14	15387.0
15	21388.5
16	27390.0
17	36618.0
18	45846.0
19	59776.0
20	73706.0
21	93053.0
22	112400.0
23	138199.0
24	163998.0
25	196846.0
26	271511.5
27	313329.0
28	369211.5
29	425094.0
30	492597.5
31	560101.0
32	635586.5
33	711072.0
34	780732.0
35	850392.0
36	898553.5
37	946715.0
38	979693.0
39	1012671.0
40	1024788.0
41	1036905.0
42	1117588.5
43	1198272.0
44	1166625.0
45	1134978.0
46	1157249.0
47	1179520.0
48	1209888.5
49	1240257.0
50	1287064.5
51	1333872.0
52	3968350.0
53	6602828.0
54	3926100.0
55	1249372.0
56	1175440.5
57	1101509.0
58	1078830.5
59	1056152.0
60	1039492.5
61	1022833.0
62	1000844.5
63	978856.0
64	961037.0
65	943218.0
66	926338.5
67	909459.0
68	879613.0
69	849767.0
70	820821.5
71	791876.0
72	759284.5
73	726693.0
74	686262.0
75	593942.0
76	542053.0
77	489759.0
78	437465.0
79	383678.5
80	329892.0
81	279870.5
82	229849.0
83	188895.5
84	147942.0
85	117177.0
86	86412.0
87	64784.0
88	43156.0
89	31611.0
90	20066.0
91	14040.0
92	8014.0
93	5408.0
94	2802.0
95	1930.5
96	1059.0
97	676.5
98	294.0
99	182.0
100	70.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002219625784487029
2	0.009398329291498957
3	7.845229065859327E-4
4	0.003830130125242704
5	3.1891175064468805E-6
6	0.018541529182482166
7	0.0
8	0.0
9	0.0
10	0.0
11	9.567352519340641E-6
12	4.2415262835743516E-4
13	0.0
14	0.0
15	0.005016481837640943
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	6.346343837829293E-4
42	0.0
43	0.0
44	0.0
45	0.002104817554254941
46	1.2756470025787522E-5
47	6.378235012893761E-4
48	0.0
49	0.002037846086619557
50	0.0
51	0.0027490192905572112
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.1356637E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	59.64430236322435
#Duplication Level	Relative count
1	100.0
2	54.97307649884184
3	23.2344299938833
4	9.464839010498661
5	4.005936205841948
6	1.886149187281278
7	0.9907046236224893
8	0.5825904719885286
9	0.39006487711452265
10++	5.019703791350387
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	5165245	16.472573254587218	TruSeq Adapter, Index 4 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA	108763	0.34685798735368206	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC	78588	0.25062636659664744	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGAAG	7782440	5.6339736	189.18048	5
GAAGA	7180570	5.4864736	198.95348	6
AAGAG	7134335	5.4511466	198.58029	7
TCTGA	6992480	5.348	195.41475	18
CTGAA	7221795	5.278376	186.63635	19
AATCT	6437235	5.1963077	201.7654	39
GTATG	6135820	5.133541	209.63025	45
ACTGA	6892080	5.0373883	184.70325	32
GTCTG	6879505	4.985194	185.16617	17
TCCAG	7719330	4.8866944	160.79587	25
GAACT	6649960	4.860424	185.67366	21
AGTCA	6577800	4.8076825	184.63316	28
AGAGC	7198205	4.7636404	171.84045	8
TCACT	6810110	4.761349	176.75945	30
TGAAC	6429600	4.699364	185.70009	20
TATGC	6089145	4.6571097	191.5678	46
GAGCA	6977140	4.6173434	171.3792	9
ATCTC	6598640	4.6134977	175.01819	40
CACTG	7188905	4.5509105	160.21288	31
AACTC	6692490	4.4715557	169.76399	22
CTCCA	7580765	4.386966	147.09906	24
TCGTA	5727765	4.3807187	191.22127	43
CGTAT	5712485	4.3690324	191.28021	44
CACAC	7841465	4.336541	143.14676	12
CTGAC	6841125	4.33075	159.78922	33
CCAGT	6755750	4.2767034	160.09282	26
CAGTC	6739170	4.2662077	160.0564	27
TCGGA	6113680	4.233724	180.2207	3
CGGAA	6396805	4.2332883	172.26137	4
CCAAT	6267860	4.187841	167.2766	37
ACCAA	6557290	4.1868773	160.14998	36
TGACC	6603570	4.180366	159.2288	34
TCTCG	6302035	4.1746626	165.70154	41
GCACA	6866735	4.1541367	156.16566	11
GTCAC	6535430	4.137231	159.91983	29
CAATC	6189220	4.1352983	166.82379	38
AGCAC	6825735	4.1293335	156.42216	10
GATCG	5887250	4.0769205	179.89943	1
ATCGG	5862930	4.0600796	180.07613	2
CGTCT	6079365	4.0271597	168.92744	16
ATGCC	6339595	4.0132575	158.77509	47
TTTTT	3740245	3.994204	4.1087255	12
CTCGT	6017860	3.9864168	165.54941	42
GACCA	6526995	3.948606	152.03786	35
CACGT	6223300	3.939638	162.06291	14
ACTCC	6787380	3.927837	146.8721	23
ACGTC	6123290	3.8763273	161.49954	15
ACACG	6037995	3.652778	154.98677	13
AAAAA	3698195	3.1477108	3.308001	34
TTTAA	1734455	1.6915433	6.6704097	47
TTTAT	1524815	1.5561172	6.7626767	12
AGGGA	2005350	1.4517412	5.113919	7
TTCCA	2065435	1.4440672	5.117988	3
CCTTT	1922910	1.4068246	5.1570272	45
ATTTA	1304830	1.2725476	6.2660494	11
GATTT	1334455	1.2330728	5.9841347	10
CTTTA	1339700	1.1316395	5.444669	46
TTATA	904170	0.88180023	5.873424	13
TATAA	913330	0.8512219	5.61683	14
GGATT	994115	0.8317275	5.09584	9
>>END_MODULE