##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV31_CGATGT_L001_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	21017840	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70436015308899	34.0	31.0	34.0	31.0	34.0
2	32.914022563688754	34.0	31.0	34.0	31.0	34.0
3	32.95720468896899	34.0	31.0	34.0	31.0	34.0
4	36.34929840554501	37.0	37.0	37.0	35.0	37.0
5	36.25710139576665	37.0	37.0	37.0	35.0	37.0
6	36.250188411368626	37.0	37.0	37.0	35.0	37.0
7	36.252344151444674	37.0	37.0	37.0	35.0	37.0
8	36.247333455769	37.0	37.0	37.0	35.0	37.0
9	38.04078406724954	39.0	38.0	39.0	35.0	39.0
10	38.041891602562394	39.0	38.0	39.0	35.0	39.0
11	38.032354133440926	39.0	38.0	39.0	35.0	39.0
12	38.02118428915626	39.0	38.0	39.0	35.0	39.0
13	37.990619350037875	39.0	38.0	39.0	35.0	39.0
14	39.49735753055499	41.0	39.0	41.0	37.0	41.0
15	39.469274816061024	41.0	39.0	41.0	36.0	41.0
16	39.46482331200542	41.0	39.0	41.0	36.0	41.0
17	39.46455744262969	41.0	39.0	41.0	36.0	41.0
18	39.49267355732083	41.0	39.0	41.0	37.0	41.0
19	39.50710672457303	41.0	39.0	41.0	37.0	41.0
20	39.47822844783289	41.0	39.0	41.0	37.0	41.0
21	39.46533402100311	41.0	39.0	41.0	37.0	41.0
22	39.44372632963235	41.0	39.0	41.0	36.0	41.0
23	39.40503638813503	41.0	39.0	41.0	36.0	41.0
24	39.392590960821856	41.0	39.0	41.0	36.0	41.0
25	39.36634606600869	41.0	39.0	41.0	36.0	41.0
26	39.30095114436117	41.0	39.0	41.0	36.0	41.0
27	39.25899740411003	41.0	39.0	41.0	36.0	41.0
28	39.21627217639872	41.0	39.0	41.0	36.0	41.0
29	39.200950478260374	41.0	39.0	41.0	36.0	41.0
30	39.202202652603695	41.0	39.0	41.0	36.0	41.0
31	39.135248008358616	41.0	39.0	41.0	36.0	41.0
32	39.08242716663558	40.0	39.0	41.0	36.0	41.0
33	39.03743005941619	40.0	39.0	41.0	35.0	41.0
34	38.99765594371258	40.0	39.0	41.0	35.0	41.0
35	38.94976833965812	40.0	39.0	41.0	35.0	41.0
36	38.908697182964566	40.0	38.0	41.0	35.0	41.0
37	38.869703356767396	40.0	38.0	41.0	35.0	41.0
38	38.82070679004122	40.0	38.0	41.0	35.0	41.0
39	38.73673755247923	40.0	38.0	41.0	35.0	41.0
40	38.68498675410984	40.0	38.0	41.0	35.0	41.0
41	38.61733379833513	40.0	38.0	41.0	35.0	41.0
42	38.582384441027244	40.0	38.0	41.0	34.0	41.0
43	38.55369048389368	40.0	38.0	41.0	34.0	41.0
44	38.48952585042041	40.0	38.0	41.0	34.0	41.0
45	38.67265090037797	40.0	38.0	41.0	35.0	41.0
46	38.74074272142142	40.0	38.0	41.0	35.0	41.0
47	38.69781285802918	40.0	38.0	41.0	35.0	41.0
48	38.66559532283051	40.0	38.0	41.0	35.0	41.0
49	38.63169545490878	40.0	38.0	41.0	35.0	41.0
50	38.58982383537033	40.0	38.0	41.0	34.0	41.0
51	38.52480235837746	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	8.0
15	29.0
16	78.0
17	229.0
18	491.0
19	1046.0
20	2111.0
21	3737.0
22	6738.0
23	11700.0
24	19050.0
25	29647.0
26	44688.0
27	64973.0
28	91996.0
29	126151.0
30	169458.0
31	222278.0
32	287039.0
33	373824.0
34	501237.0
35	688997.0
36	986629.0
37	1537835.0
38	3013126.0
39	1.279745E7
40	37294.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.970476503778269	23.37095346337164	32.791942673495456	27.866627359354634
2	21.167450762462202	26.591318098887573	27.889024357574137	24.352206781076084
3	22.440429755708852	26.53909265463199	27.749410164038817	23.27106742562034
4	21.889499755649595	24.723226953636324	28.907506984006098	24.47976630670798
5	21.658941402961684	26.563323333414374	29.498972705897376	22.278762557726566
6	22.51104843915159	27.285066766512035	27.05237237118339	23.151512423152983
7	22.8316991660418	26.865367706672043	28.32922412579028	21.973709001495873
8	22.42651005050947	27.327242000129417	27.63007521229584	22.616172737065273
9	21.818897660273368	26.718149914548782	28.48914541170739	22.973807013470463
10	22.280220041640817	26.951247131008703	27.334711844794708	23.433820982555773
11	21.97623171440223	26.823514063458187	27.743156658493767	23.457097563645814
12	22.297529886718298	27.0788576586653	27.27601453264738	23.347597921969022
13	22.007285239586942	27.27819794993206	27.631378866715135	23.083137943765866
14	22.234435127491693	27.329254576112483	27.400513087929113	23.03579720846671
15	22.06427332380663	27.226759960037516	27.63394542238836	23.075021293767488
16	22.22509068486581	27.19647213985833	27.570197508402384	23.008239666873475
17	22.13622808052588	27.143688409465483	27.68185027576573	23.03823323424291
18	22.249236838799803	27.204731789755748	27.59998648766952	22.946044883774928
19	22.135133772071725	27.177883169726293	27.638468082352897	23.048514975849088
20	22.324101810652284	27.150154345070664	27.549153481042772	22.97659036323428
21	22.19639125618998	27.167430145057725	27.672258424271952	22.963920174480347
22	22.217620840200517	27.195482504386746	27.589585799492237	22.997310855920496
23	22.149845083985795	27.28461154904595	27.667448224936532	22.89809514203172
24	22.28466864340008	27.248932335577774	27.556656630747973	22.909742390274168
25	22.14158067622553	27.300374348648575	27.670883401909997	22.8871615732159
26	22.28160457972846	27.415409956494102	27.471076951770495	22.831908512006944
27	22.244792994903378	27.359866665651655	27.536040811044334	22.859299528400634
28	22.347239297663318	27.437405556422544	27.454219843713723	22.761135302200415
29	22.263405754349638	27.27975377108209	27.59333499541342	22.863505479154853
30	22.34602128477522	27.3197483661499	27.518807831822873	22.81542251725201
31	22.227493405602097	27.31738846617921	27.575959280306634	22.87915884791206
32	22.33882263829204	27.381110523250722	27.496783684717364	22.78328315373987
33	22.25911416206423	27.352877365133622	27.510657612770867	22.877350860031285
34	22.298219036780186	27.41648999135972	27.503858626766593	22.781432345093503
35	22.283755133734008	27.483409332262497	27.509415810568544	22.723419723434947
36	22.34310947271461	27.528204610940037	27.49482344522558	22.633862471119773
37	22.275181464888874	27.464611016165314	27.569483828975766	22.690723689970042
38	22.423236640872705	27.443224422680924	27.46878366187962	22.66475527456675
39	22.33101974322766	27.436006744746365	27.563455616752247	22.66951789527373
40	22.42381710013969	27.405580211858116	27.49000373016447	22.680598957837724
41	22.34539004905588	27.418556124981347	27.574306069409044	22.661747756553726
42	22.422061448750206	27.400265679061214	27.465148654666706	22.712524217521874
43	22.315623299064033	27.406945718494384	27.586302874129785	22.691128108311794
44	22.352901154447842	27.488314688854803	27.476967185971535	22.68181697072582
45	22.369835983892763	27.46761526539907	27.545246228753367	22.617302521954798
46	22.360485187821393	27.524702823886756	27.50242651005051	22.612385478241343
47	22.308830250643243	27.559242736663347	27.50416054444063	22.62776646825278
48	22.36419632083982	27.527471900062046	27.502859475569323	22.605472303528813
49	22.389796360761512	27.455117587533813	27.52197183243341	22.633114219271267
50	22.4094483543504	27.436758487075743	27.442396554546043	22.71139660402782
51	22.380942230552318	27.45149528239539	27.47520915454431	22.692353332507977
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.8371038631329
2	45.51965754353829
3	45.71149718132919
4	46.369266062357575
5	43.937703960688246
6	45.662560862304574
7	44.80540816753768
8	45.04268278757475
9	44.79270467374383
10	45.71404102419659
11	45.43332927804804
12	45.64512780868732
13	45.090423183352804
14	45.270232335958404
15	45.139294617574116
16	45.23333035173928
17	45.17446131476879
18	45.19528172257473
19	45.18364874792081
20	45.30069217388657
21	45.160311430670326
22	45.214931696121006
23	45.047940226017516
24	45.19441103367425
25	45.02874224944143
26	45.1135130917354
27	45.10409252330401
28	45.10837459986374
29	45.126911233504494
30	45.16144380202723
31	45.106652253514156
32	45.12210579203192
33	45.13646502209552
34	45.07965138187369
35	45.007174857168955
36	44.97697194383438
37	44.96590515485892
38	45.08799191543946
39	45.000537638501385
40	45.10441605797742
41	45.0071378056096
42	45.13458566627208
43	45.00675140737583
44	45.03471812517366
45	44.98713850584756
46	44.97287066606274
47	44.93659671889602
48	44.96966862436863
49	45.02291058003278
50	45.120844958378214
51	45.07329556306029
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	102.0
1	134.0
2	166.0
3	246.0
4	326.0
5	451.5
6	577.0
7	945.5
8	1314.0
9	1992.5
10	2671.0
11	4091.5
12	5512.0
13	8208.0
14	10904.0
15	16031.5
16	21159.0
17	29776.0
18	38393.0
19	52025.0
20	65657.0
21	85670.5
22	105684.0
23	134620.0
24	163556.0
25	203237.5
26	295265.0
27	347611.0
28	419612.0
29	491613.0
30	583350.5
31	675088.0
32	779617.5
33	884147.0
34	986319.0
35	1088491.0
36	1165171.0
37	1241851.0
38	1301631.5
39	1361412.0
40	1384082.0
41	1406752.0
42	1443653.5
43	1480555.0
44	1505358.0
45	1530161.0
46	1542411.0
47	1554661.0
48	1544373.0
49	1534085.0
50	1487442.5
51	1440800.0
52	1364727.5
53	1288655.0
54	1178727.0
55	1068799.0
56	961755.0
57	854711.0
58	753075.5
59	651440.0
60	563540.5
61	475641.0
62	403356.5
63	331072.0
64	276507.5
65	221943.0
66	185113.5
67	148284.0
68	120605.5
69	92927.0
70	76761.0
71	60595.0
72	51026.0
73	41457.0
74	35194.5
75	24342.5
76	19753.0
77	16766.5
78	13780.0
79	11601.5
80	9423.0
81	7750.0
82	6077.0
83	4907.5
84	3738.0
85	2959.5
86	2181.0
87	1687.0
88	1193.0
89	898.0
90	603.0
91	425.5
92	248.0
93	185.0
94	122.0
95	92.0
96	62.0
97	43.5
98	25.0
99	18.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0025977931128983757
2	0.009101791620832588
3	6.28037895425981E-4
4	0.004239255794125371
5	1.4273588532408658E-5
6	0.019088545730674512
7	0.0
8	0.0
9	0.0
10	0.0
11	4.757862844136219E-6
12	2.8071390780403695E-4
13	0.0
14	0.0
15	0.005362111425341519
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	8.373838605679747E-4
42	0.0
43	0.0
44	0.0
45	0.0021553118683937072
46	0.0
47	7.517423293735227E-4
48	0.0
49	0.0022647427138088403
50	0.0
51	0.002883264883546549
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.101784E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	7.269249163079861
#Duplication Level	Relative count
1	100.0
2	2.343114476055784
3	0.5609225714179378
4	0.3945066805612416
5	0.3227298586262448
6	0.2536114375036553
7	0.23181255084191554
8	0.18023957508121413
9	0.14993380581977106
10++	1.8991615403837667
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTGTG	2572050	3.1259625	3.7439296	4
CACAC	2763880	3.1224473	3.7259095	12
ACACA	3140730	3.006174	3.2755628	8
>>END_MODULE