FastQCFastQC Report
Mon 6 Nov 2023
H7YLTDRX3_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7YLTDRX3_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50034535
Sequences flagged as poor quality0
Sequence length90
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG4959150.9911454158612646Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGACCTCAAATCAGGTA808640.1616163715721551Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG804360.16076096240326807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC795800.15905014406549398Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCA698130.13952962688670936No Hit
GTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGG611810.1222775429011182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA610260.12196775687032967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGG577350.11539029991984537No Hit
GGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAT547060.10933648129237136No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG514070.10274303538545927No Hit
GCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATA512280.10238528248538734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)