FastQCFastQC Report
Mon 6 Nov 2023
H7YLTDRX3_n02_WILD_YEAST_WTA_30M.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7YLTDRX3_n02_WILD_YEAST_WTA_30M.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430759524
Sequences flagged as poor quality0
Sequence length90
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG38597640.8960368337671393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCA11018330.25578842454102074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC10495730.24365636544811486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGG9827640.22814678381899225No Hit
GGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAT8833320.20506383510629006No Hit
CAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGG8584870.19929611585326204No Hit
GCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATA8399430.19499116170441305No Hit
CAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAG8255040.19163917545790585No Hit
GTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGA8073390.1874222054345106No Hit
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG7313390.16977895072611326No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAA7282160.1690539522464511No Hit
GGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGA7200360.1671549808844157No Hit
CTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGC7142360.16580852197245904No Hit
CCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGT6685480.15520214011565303No Hit
GGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAG6587840.1529354461818005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA6335950.1470878679864081Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC6309140.14646547896176057No Hit
GGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTG6214680.14427260811997739No Hit
GTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCT6203410.14401097722449893No Hit
GAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTT6144940.14265360735239366No Hit
CCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATG5852490.13586443651098473No Hit
ATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAA5786410.1343304019437072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGACCTCAAATCAGGTA5612840.13030100757563284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAAC5433770.12614393175901087No Hit
CTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTG5371470.12469764916909881No Hit
TGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTG5126100.11900143152725742No Hit
CTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCT4976540.11552942471911544No Hit
CAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTT4896610.11367386504958622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCATATCAATAAGCGGAGGA4875890.11319285421069412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG4869430.11304288654567275No Hit
GCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAA4742310.11009182004760502No Hit
GGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAAT4723920.10966489971327947No Hit
GCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTAT4704610.10921662175483321No Hit
GGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGA4530290.10516981628013872No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG4441390.10310601977543275No Hit
GGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCG4363880.1013066399432645No Hit
GAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGAC4331900.1005642303569822No Hit
CTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCG4326850.10044699557240665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)