FastQCFastQC Report
Mon 6 Nov 2023
H7YLTDRX3_n02_WILD_YEAST_WTA_0M.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7YLTDRX3_n02_WILD_YEAST_WTA_0M.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590696377
Sequences flagged as poor quality0
Sequence length90
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG126279822.137812671906738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGACCTCAAATCAGGTA21609870.3658371854208952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC16015020.27112101281772377Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA14290050.2419187006457634Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGA12274650.20779964932813527Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCA12129790.20534728961102128No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCATATCAATAAGCGGAGGA11567070.19582090648238393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGG10689100.1809576021828216No Hit
CAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGG10612900.17966759934943702No Hit
GCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGGAG10331760.17490813220274753Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAG9847580.16671136616773255No Hit
GGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAT9167790.1552030849852326No Hit
GCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATA8457650.14318100346161425No Hit
CAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAG8029800.13593785763138344No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAA7935820.1343468541368758No Hit
CCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGT7572010.12818785242016137No Hit
ATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAA7455300.1262120488678738No Hit
GAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTT7270260.12307947505830055No Hit
GTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCT7259140.12289122267631582No Hit
GGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGA7044730.11926143911324516No Hit
GGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAAT7018510.11881755624852934No Hit
CCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATG7009970.11867298112783245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACCACATCGATTCAATC6693290.11331185124231768Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGA6579910.11139242182959927No Hit
GGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAG6546600.11082851114219718No Hit
GCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGA6283700.10637783207530999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATATCAATAAGCGGAGGAAA6261880.10600843756317808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTT6238260.10560857054317094No Hit
TGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTG6122850.10365477491323771No Hit
CTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCT6017370.10186908595174946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)