FastQCFastQC Report
Wed 30 Aug 2023
H7Y7KDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7Y7KDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919715166
Sequences flagged as poor quality0
Sequence length8
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG17860141819.419209838277258Illumina Single End PCR Primer 1 (100% over 8bp)
GTGACGCA500899225.44624290777434No Hit
AGAGGATA482847635.24996920622705No Hit
AGCTAGAA475491645.169988030837799No Hit
TCTTACGC463278025.037190177203189No Hit
CTAGTCGA461204025.014639717270901No Hit
CTTAATAG449981874.8926220490312105No Hit
TATGCAGT421102124.5786145055261604No Hit
TAAGGCTC391343614.255052264735624No Hit
CTCCTTAC385475134.191244683682862No Hit
ACTCTAGG376135494.089695417722404No Hit
CACTAGAC362707863.9436977165167244No Hit
TACTCCTT316024383.4361114362661276ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
CGGAGAGA298018723.240337128462661No Hit
AGGCTTAG288863553.1407935921761236No Hit
TCTACTCT265912102.8912440484862025No Hit
TCGCATAA256705662.7911430570016282No Hit
ATAGAGAG189105432.0561303867854237ABI Solid3 Adapter B (100% over 8bp)
ATAGCCTT167839681.8249093437260988No Hit
ATTAGACG141898301.5428504959545268No Hit
CAGATAGT101142371.0997140608204345No Hit
GCGATCTA95538721.0387859582169814No Hit
GGGGGGGG25189730.2738862088091304No Hit
CGATCTCG15638510.1700364480017719Illumina Paired End PCR Primer 2 (100% over 8bp)
TGATCTCG14028470.15253059336851255Illumina PCR Primer Index 5 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)