FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs41_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs41_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8151417
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG10250.013.170258511
CACACGT12000.011.6245112
ACGTCTG12150.010.36980615
ACACGTC11750.010.33992713
GATCGGA11500.010.1780411
AGCACAC14750.010.06749610
CGTCTGA12200.09.95841416
CACGTCT12950.09.55552414
GTATGCC13000.08.48025645
TCCAGTC17050.08.4453425
GAACTCC16300.08.41973621
CACTAGC14000.08.356585531
GAGCACA18050.07.85299979
CGTATGC12950.07.818059444
AGTCACT17600.07.670037328
AGAGCAC18300.07.4999148
CAGTCAC16800.07.49959227
CCAGTCA18850.07.40017326
TCGGAAG20200.07.3538823
GTCACTA18950.07.00489629