FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs37_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs37_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9174449
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18100.024.61241711
GTCACAC18650.023.76555629
ACGTCTG20450.022.22345415
CACACGT20450.022.00401912
ACACGTC20700.021.62957613
CACGTCT22100.020.86987314
GATCGGA20700.020.7698961
CGTCTGA22250.020.22347816
CACACTG22400.019.98761231
TATCTCG22200.019.96477341
CAGTCAC23800.019.09567527
AGCACAC25100.018.55534610
TCCAGTC24600.018.47457525
GAGCACA25500.017.8232129
CCAGTCA25850.017.75529326
TCGTATG26150.017.29329945
GAACTCC26700.017.18986121
AGAGCAC27500.016.7727038
ACACTGA27750.016.45844832
TCACACT26950.016.44638330
Produced by FastQC (version 0.11.2)