FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs36_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs36_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6147142
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG109820.1786521280946495TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18600.031.93353311
ACACGTC19600.030.4188113
AGTCACG19400.029.9208528
TCACGTG20100.029.43850130
CACACGT20850.028.81095512
CACGTGA20500.028.75410731
GATCGGA19750.028.2673051
GTCACGT21100.027.72342729
CACGTCT21550.027.56189714
CGATCTC18700.027.1906340
ACGTCTG21850.027.1832515
GATCTCG19400.026.90535241
CGTCTGA22000.026.793616
AGCACAC22450.026.4571810
GAACTCC22550.025.6412421
CCAGTCA23600.024.7866226
GAGCACA24400.024.6193989
AGAGCAC24650.024.4615758
CAGTCAC24250.024.30779527
TCGTATG23750.024.06149345
Produced by FastQC (version 0.11.2)