FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs34_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs34_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307330
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG684950.8245128097716113TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG104750.040.25060335
GTCCCGA99600.039.77927436
TCACCCG106750.039.62247530
CCCGTCC107650.039.43774833
GCACACG110750.039.18895311
ACCCGTC107600.039.18401332
CCCGATC87250.039.09224338
CCGTCCC108650.038.95075634
ACGTCTG111950.038.5461815
ACACGTC112400.038.53289813
CACCCGT110000.038.49271431
CACGTCT112650.038.42718514
CACACGT112900.038.3826412
GATCGGA111550.038.2396161
TCGTATG107450.038.10826545
CGTCTGA114050.037.81670416
GTCACCC112050.037.7882529
AGCACAC115100.037.74719210
CGATCTC89450.037.40114240
CCGATCT89200.037.35485539
Produced by FastQC (version 0.11.2)