FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs33_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs33_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6388947
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG8950.012.82067511
ACACGTC9950.011.75819313
GATCGGA9350.011.5548231
CACACGT10100.011.13804412
CACGTCT10600.010.61274914
ACGTCTG10650.010.5628415
CGTCTGA11300.010.15434816
AGCACAC11750.09.76568810
TCGGAAG15550.08.6842183
GAGCACA13850.08.6099389
CAGTCAC13350.08.59499827
GAACTCC12100.08.36727521
TCCAGTC14500.07.758102425
ATCTCGT12800.07.73391942
TCACATG14600.07.705025730
AGTCACA15500.07.54794228
GTCTGAA17050.07.5216117
AGAGCAC16150.07.3838128
CCAGTCA14850.07.27229926
ACATGTC15800.07.11983332