FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs32_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs32_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9053273
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG607870.6714367279104474TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG85400.038.5428611
ACACGTC85950.038.3481513
CACACGT87100.037.8420412
GATCGGA83550.037.772851
ACGTCTG87750.037.61300715
CACGTCT88900.037.1011414
GTTCCGT86800.036.9101135
CCGTATC84050.036.61894638
CGTCTGA89950.036.6180316
GTCACAG88700.036.4993429
CAGTCAC89300.036.279327
AGCACAC91100.036.23087310
TATCTCG83650.036.14834241
TCGTATG85050.036.08197845
AGTTCCG89250.036.0733534
GAGCACA92050.035.9058389
GAACTCC91800.035.58598721
TCCAGTC91100.035.4146925
CCAGTCA91500.035.33363326
AGTCACA91950.035.28266528
Produced by FastQC (version 0.11.2)