FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs31_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs31_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12317359
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16500.010.49948111
CACACGT20150.08.70924712
TGGGGGG14050.08.4907445
ACGTCTG21150.08.40363515
ACACGTC20450.08.36137713
CACGTCT22200.07.90485414
CGTCTGA22550.07.682328716
GATCGGA21800.07.53822371
CACAGTC25700.07.44108531
AGCACAC25200.07.321155510
GAGCACA25300.07.29224829
CAGTCAC25550.07.2206927
GTCACAG27250.06.935352329
TCGTATG26650.06.922566445
AGAGCAC29900.06.62185348
GAACTCC27450.06.47493221
ATCTCGT28400.06.416754242
TCCAGTC27850.06.301227625
CCAGTCA30100.06.278682726
TCGGAAG33450.06.19062333
Produced by FastQC (version 0.11.2)