FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs30_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs30_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9471656
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC396140.4182373177404247TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC61250.035.70363245
GCACACG62900.035.590111
CACACGT63950.035.00574512
ACACGTC64600.034.58386213
GATCGGA63450.033.631721
CGTCTGA66300.033.62940616
ACGTCTG66200.033.6120615
CACGTCT67800.032.91857514
AGCACAC68750.032.7909610
CGTATGC67100.032.59069444
GAGCACA70550.031.9224439
CAGTCAC69450.031.84459727
TCCAGTC69450.031.81253825
AGTCACC70700.031.37704328
AGAGCAC72350.031.2845578
GTCACCT71200.031.28309629
CCAGTCA71500.031.02629726
CTCGTAT67450.030.42041242
TCGTATG71900.030.3212543
TCGGAAG74650.030.2406233
Produced by FastQC (version 0.11.2)