FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs28_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs28_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300288
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC2132532.5692241040310893TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG249300.043.5893311
GTCACGC245950.043.43293429
ACACGTC250150.043.3962413
GATCGGA242750.043.3490071
AGTCACG248200.043.21143728
TCACGCC247200.043.15870730
CACACGT251350.043.15325512
CACGTCT252250.043.00846514
ACGTCTG251850.042.9603815
CACGCCA248750.042.92569431
CGTCTGA252300.042.84834716
GTATGCC247600.042.78886445
AGCACAC254400.042.77790510
GAGCACA255900.042.518629
TATCTCG247300.042.25018739
CGTATGC250850.042.16274344
TCGGAAG258750.042.128173
CAGTCAC254650.042.0904327
CCAGTCA255500.041.96801826
AGAGCAC259900.041.9260988