FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs26_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs26_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8014388
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC103660.12934237773364604TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20550.027.48045511
ACACGTC22900.024.56170313
CACACGT22800.024.37339412
GTATGCC22250.024.36918345
ACTGACC22850.024.2217532
ACGTCTG23250.024.09503615
AGCACAC23500.024.03093110
GATCGGA22500.023.7117521
CACGTCT23800.023.3494514
CGTCTGA24200.023.149316
CGTATGC24650.022.27019944
GAGCACA26500.021.8199989
TCCAGTC25800.021.62661725
CAGTCAC25850.021.58478527
CCAGTCA26950.020.78725826
CACTGAC28200.020.10518531
AGAGCAC29150.019.6821178
CTCGTAT25950.019.50726342
CTGACCA28550.019.4648133
TCGTATG28100.019.21569643
Produced by FastQC (version 0.11.2)