FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs25_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs25_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8354467
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT7850.010.89107831
AGTCACG9000.010.24939628
GCACACG9550.09.6592911
ACGAGTG9400.09.57390432
CGAGTGG9000.09.49955433
TCACGAG10750.08.3716530
CACACGT10900.08.0500812
GATCGGA11400.07.8981481
ACACGTC11100.07.702340613
CGTCTGA12350.07.469285516
GTCACGA11750.07.46764529
AGCACAC13550.07.13984410
ACGTCTG12300.07.13376915
TATCTCG13650.07.08745241
TGGGGGG8804.7293724E-116.90564785
CAGTCAC14650.06.757281327
CACGTCT13250.06.452488414
ATCTCGT17950.06.26700342
GAGCACA16100.06.14877949
TCCAGTC16200.06.11075125
Produced by FastQC (version 0.11.2)