FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs24_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs24_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8060352
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12300.022.13274811
ACACGTC14200.019.01264813
ACGTCTG14600.018.64619815
CACACGT14800.018.3938812
CACGTCT15300.017.64605514
GAACTCC16150.016.43848821
TATCTCG15800.016.37534541
AGCACAC17400.015.77496410
CGTCTGA17450.015.72976316
CAGTCAC18350.015.32629427
TCCAGTC17750.015.21068425
AGTCACA18700.014.91912128
GTCACAT19150.014.21607829
GAGCACA19900.013.5671529
GATCGGA18900.013.4587951
CCAGTCA20300.013.1891526
CTCGTAT19650.013.05236744
TCGTATG21250.012.81071845
GTCTGAA21800.012.38461617
TCTGAAC24300.011.75851618
Produced by FastQC (version 0.11.2)