FastQCFastQC Report
Mon 6 Feb 2017
H7V7CAFXX_n01_vs23_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs23_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9373539
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC13100.06.35453213
GTCACAC12600.06.2495129
ACGTCTG14600.06.009835215
GCACACG13551.8189894E-125.811507711
CACGTCT14850.05.757247414
CAGTCAC15101.8189894E-125.512783527
CACACGT15503.6379788E-125.370604512
CGTCTGA14852.3646862E-115.30264316
TGGGGGG10051.4522539E-65.15148835
GATCGGA14057.876224E-105.1271621
GTATGCC14553.8562575E-105.102720345
Produced by FastQC (version 0.11.2)