FastQCFastQC Report
Tue 11 Apr 2017
H7V7CAFXX_n01_vs42_170203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7V7CAFXX_n01_vs42_170203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8371463
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT6600.011.93086231
GTCACGG6750.011.66573229
GCACACG7900.011.39176611
TCACGGC6950.011.00631330
AGTCACG8750.010.28489128
CGGCTAC7700.09.93438933
CACACGT10950.08.42413412
ACACGTC10950.08.21866713
ACGTCTG11500.08.21678315
ACGGCTA9400.08.13759832
GATCGGA10650.07.60934261
CACGTCT11950.07.53091314
GTATGCC12750.07.411171445
CGTCTGA13400.07.387468316
CGTATGC12700.07.263198444
AGCACAC13450.07.192947410
GGCTACA12550.06.81232634
CAGTCAC14350.06.7416227
CATCTCG10800.06.66637239
TCCAGTC15750.06.142365525
Produced by FastQC (version 0.11.2)